FastQCFastQC Report
Thu 2 Feb 2017
SRR4062614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770053
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33010.18649159092976314No Hit
TATCAACGCAGAGTACTTTTTTTTT21250.12005290237071997No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19450.10988371534637663No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18370.10378220313177064No Hit
GCGCAAGACGGACCAGAGCGAAAGC18270.1032172482970849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG400.00528198614.2489815
CGCATCG2950.013.84881913
TCGCCAG3300.012.95838517
CGTCGTA3050.012.77210810
ATCGCCA3150.012.66970416
GAGCGTT751.4777908E-512.6675547
CGCCAGT3250.012.572618
CGAGCCG3250.012.571177515
GCATCGC3300.012.38000414
CCGCATC3250.012.27882412
CAAGACG2800.012.2116864
ACGGTAT3050.012.1490789
AGCGCTC550.003065824712.0924410
GTGTTAT550.003084715512.0825251
AAGACGG2800.011.8741525
TACCGTC3450.011.8414097
CCGTCGT3300.011.8045249
CGTTATT1052.740053E-711.7554112
AACGGAC1302.6084308E-911.69411815
TGCGTAG658.017767E-411.69345713
Produced by FastQC (version 0.11.5)