##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062614_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1770053
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176325228679595	32.0	32.0	32.0	32.0	32.0
2	30.782163019977368	32.0	32.0	32.0	32.0	32.0
3	30.766478743856823	32.0	32.0	32.0	32.0	32.0
4	30.8562127800693	32.0	32.0	32.0	32.0	32.0
5	30.623074563304037	32.0	32.0	32.0	32.0	32.0
6	34.28014415387562	36.0	36.0	36.0	32.0	36.0
7	34.16475890834907	36.0	36.0	36.0	32.0	36.0
8	34.155459751770145	36.0	36.0	36.0	32.0	36.0
9	34.33480014440246	36.0	36.0	36.0	32.0	36.0
10	33.964218585545176	36.0	36.0	36.0	32.0	36.0
11	34.3292466383775	36.0	36.0	36.0	32.0	36.0
12	34.060658635645375	36.0	36.0	36.0	32.0	36.0
13	34.209195995826114	36.0	36.0	36.0	32.0	36.0
14	34.107212608887984	36.0	36.0	36.0	32.0	36.0
15	34.03713674110323	36.0	36.0	36.0	32.0	36.0
16	34.047627952383344	36.0	36.0	36.0	32.0	36.0
17	33.965045114468325	36.0	36.0	36.0	32.0	36.0
18	33.975587736638396	36.0	36.0	36.0	32.0	36.0
19	33.96739589153545	36.0	36.0	36.0	32.0	36.0
20	33.95676118172733	36.0	36.0	36.0	32.0	36.0
21	33.94124413223785	36.0	36.0	36.0	32.0	36.0
22	33.88765929607757	36.0	36.0	36.0	32.0	36.0
23	33.85703761412794	36.0	36.0	36.0	32.0	36.0
24	33.8280317030055	36.0	36.0	36.0	32.0	36.0
25	33.370545401747854	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	79.0
5	177.0
6	335.0
7	85.0
8	256.0
9	322.0
10	222.0
11	63.0
12	129.0
13	116.0
14	343.0
15	397.0
16	625.0
17	810.0
18	1116.0
19	1568.0
20	2239.0
21	3598.0
22	5073.0
23	7784.0
24	11056.0
25	15723.0
26	21938.0
27	28547.0
28	38602.0
29	50959.0
30	65453.0
31	89115.0
32	127732.0
33	183659.0
34	401595.0
35	710326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03119083388939	17.80959607573488	11.065584653691626	26.093628436684103
2	17.011196051523193	20.20842949628785	36.57866473370889	26.201709718480064
3	18.972457740833043	23.875758416380833	28.54922481780775	28.602559024978376
4	12.637242487578886	15.382233500530296	36.13889722330993	35.84162678858088
5	14.634153233851091	36.39404282110886	33.75054610110656	15.22125784393349
6	33.959503020909814	35.54408877840387	16.92139077725192	13.575017423434394
7	30.0165196183188	30.64751024137186	20.96244585330443	18.373524287004905
8	28.034749250893864	32.715708355551584	19.827011475078898	19.42253091847565
9	27.261581336850128	14.420656051430885	19.009928314358167	39.30783429736082
10	15.902055047511412	26.596266398776848	31.75987659872696	25.741801954984783
11	37.04623836376276	21.380696785550448	22.394860229395864	19.178204621290927
12	24.339266984903187	24.109075398069084	28.77095003671525	22.78070758031248
13	29.12337981896736	19.59755817492129	25.469013914013455	25.810048092097894
14	23.22668306366716	20.026496165013864	25.192456399270736	31.554364372048244
15	25.06289678140427	27.273164341597393	22.434410874355763	25.22952800264257
16	25.611297731166445	26.029283920088282	23.848233117515207	24.511185231230073
17	23.815433627797976	26.038484230199437	25.399852136023544	24.746230005979044
18	24.689457683765585	24.981190396183997	26.422769746689795	23.906582173360622
19	25.289626290300042	25.459167770260827	25.546286181718823	23.7049197577203
20	25.16754796215973	24.766835685340176	25.385665768401427	24.67995058409867
21	26.30564867497632	24.633628663006256	24.6662691727639	24.39445348925352
22	25.4131920436272	24.811835653395107	25.05678031027455	24.71819199270315
23	24.200295554016684	24.66976477883236	25.63337044731399	25.496569219836967
24	24.349491275163988	25.558720451671846	25.40937004109965	24.682418232064514
25	24.487630090110578	24.93379879245377	25.51454786031927	25.06402325711638
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	295.0
2	680.5
3	1066.0
4	1066.0
5	1066.0
6	2682.0
7	4298.0
8	4298.0
9	4298.0
10	4653.0
11	5008.0
12	5008.0
13	5008.0
14	5754.5
15	6501.0
16	6501.0
17	6501.0
18	10441.0
19	14381.0
20	14381.0
21	14381.0
22	22261.5
23	30142.0
24	30142.0
25	30142.0
26	43458.0
27	56774.0
28	56774.0
29	56774.0
30	68493.0
31	80212.0
32	80212.0
33	80212.0
34	97992.5
35	115773.0
36	115773.0
37	115773.0
38	130024.0
39	144275.0
40	144275.0
41	144275.0
42	162608.5
43	180942.0
44	180942.0
45	180942.0
46	205264.0
47	229586.0
48	229586.0
49	229586.0
50	230545.0
51	231504.0
52	231504.0
53	231504.0
54	214651.0
55	197798.0
56	197798.0
57	197798.0
58	180842.5
59	163887.0
60	163887.0
61	163887.0
62	145336.5
63	126786.0
64	126786.0
65	126786.0
66	105349.5
67	83913.0
68	83913.0
69	83913.0
70	63859.5
71	43806.0
72	43806.0
73	43806.0
74	34527.5
75	25249.0
76	25249.0
77	25249.0
78	20573.0
79	15897.0
80	15897.0
81	15897.0
82	11267.5
83	6638.0
84	6638.0
85	6638.0
86	4922.0
87	3206.0
88	3206.0
89	3206.0
90	2138.5
91	1071.0
92	1071.0
93	1071.0
94	662.0
95	253.0
96	253.0
97	253.0
98	522.5
99	792.0
100	792.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008022358652537523
2	0.003050756107303002
3	0.004632629644423077
4	0.016044717305075045
5	0.03909487456025328
6	0.04994200738621951
7	0.0704498678853119
8	0.09107071935134145
9	0.10039247412365619
10	0.12214323525905721
11	0.12383809976311444
12	0.135306682907235
13	0.12400758621352015
14	0.12711483780429175
15	0.11734111916422842
16	0.11592873207751406
17	0.12496800943248591
18	0.13214293583299483
19	0.1324254132503377
20	0.1243465591143316
21	0.13056106229587475
22	0.1533852376171787
23	0.1426510957581496
24	0.1354761693576407
25	0.13197344938258912
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1770053.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.65657498406061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14808262631831	43.54128180251796
2	11.688391734539799	12.543181350948181
3	3.1132227852021157	5.011346154488501
4	1.267387213940394	2.720146283145297
5	0.687878095928548	1.8454591317041487
6	0.4053337027652021	1.3049290929592803
7	0.2824616017497542	1.0609145480082487
8	0.2048160054730872	0.8791780284481978
9	0.14596657556367493	0.7048859856208987
>10	0.8490425166646078	8.461574527273347
>50	0.09399755140647946	3.5471821894486872
>100	0.09736811089507287	11.082606072030034
>500	0.012249813343059423	4.31761288486957
>1k	0.003801666209914993	2.9797019485378557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3301	0.18649159092976314	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2125	0.12005290237071997	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1945	0.10988371534637663	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1837	0.10378220313177064	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1827	0.1032172482970849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.64954834685741E-5	0.0
8	0.0	0.0	0.0	1.129909669371482E-4	0.0
9	0.0	0.0	0.0	2.259819338742964E-4	0.0
10	0.0	0.0	0.0	2.259819338742964E-4	0.0
11	0.0	0.0	0.0	2.259819338742964E-4	0.0
12	0.0	0.0	0.0	2.259819338742964E-4	1.6948645040572232E-4
13	0.0	0.0	0.0	3.3897290081144464E-4	2.259819338742964E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	40	0.005281986	14.248981	5
CGCATCG	295	0.0	13.848819	13
TCGCCAG	330	0.0	12.958385	17
CGTCGTA	305	0.0	12.772108	10
ATCGCCA	315	0.0	12.669704	16
GAGCGTT	75	1.4777908E-5	12.667554	7
CGCCAGT	325	0.0	12.5726	18
CGAGCCG	325	0.0	12.5711775	15
GCATCGC	330	0.0	12.380004	14
CCGCATC	325	0.0	12.278824	12
CAAGACG	280	0.0	12.211686	4
ACGGTAT	305	0.0	12.149078	9
AGCGCTC	55	0.0030658247	12.09244	10
GTGTTAT	55	0.0030847155	12.082525	1
AAGACGG	280	0.0	11.874152	5
TACCGTC	345	0.0	11.841409	7
CCGTCGT	330	0.0	11.804524	9
CGTTATT	105	2.740053E-7	11.755411	2
AACGGAC	130	2.6084308E-9	11.694118	15
TGCGTAG	65	8.017767E-4	11.693457	13
>>END_MODULE