FastQCFastQC Report
Thu 2 Feb 2017
SRR4062614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770053
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18320.10349972571442777No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18100.10225682507811913No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17760.1003359786401876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG609.75496E-625.6671265
TTGTACG600.007412661318.3336624
TAGCTCG2150.017.3951736
CCTATTC6200.017.0325643
GTCCTAT5850.016.9272061
GTATCAA35200.016.7540871
CGCAATA5400.016.70353336
TCTATAC1854.2200554E-1016.6489493
GTATTAC1602.1245796E-816.5040261
CTATTCC6400.016.5002984
TAGGACG2151.2732926E-1116.3723874
ACCGAGT1351.0862586E-616.296138
CATTCCG2051.05501385E-1016.0973979
GATTTCG1105.6241035E-515.99983641
TCCATTA6150.015.73967558
CAAGACG7000.015.7145684
ACCGTCG4700.015.446658
GAACCGT1004.9446296E-415.4002776
AAGACGG7050.015.2910565
CGGTCCA6100.015.14738510
Produced by FastQC (version 0.11.5)