##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062614_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1770053
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319732798961386	32.0	32.0	32.0	32.0	32.0
2	31.44418782940398	32.0	32.0	32.0	32.0	32.0
3	31.52296795632673	32.0	32.0	32.0	32.0	32.0
4	31.61510531040596	32.0	32.0	32.0	32.0	32.0
5	31.561183196209377	32.0	32.0	32.0	32.0	32.0
6	35.15656932306547	36.0	36.0	36.0	36.0	36.0
7	35.171474526468984	36.0	36.0	36.0	36.0	36.0
8	35.11487678617533	36.0	36.0	36.0	36.0	36.0
9	35.23854370462353	36.0	36.0	36.0	36.0	36.0
10	35.123278229521944	36.0	36.0	36.0	36.0	36.0
11	35.246924809596095	36.0	36.0	36.0	36.0	36.0
12	35.159382233187365	36.0	36.0	36.0	36.0	36.0
13	35.20026292998006	36.0	36.0	36.0	36.0	36.0
14	35.161014952659606	36.0	36.0	36.0	36.0	36.0
15	35.13411858288989	36.0	36.0	36.0	36.0	36.0
16	35.13823710363475	36.0	36.0	36.0	36.0	36.0
17	35.112848033363974	36.0	36.0	36.0	36.0	36.0
18	35.129739053011406	36.0	36.0	36.0	36.0	36.0
19	35.109498981103954	36.0	36.0	36.0	36.0	36.0
20	35.10770581445866	36.0	36.0	36.0	36.0	36.0
21	35.10036931097543	36.0	36.0	36.0	36.0	36.0
22	35.08494547903368	36.0	36.0	36.0	36.0	36.0
23	35.04895107660618	36.0	36.0	36.0	36.0	36.0
24	35.02425125123372	36.0	36.0	36.0	36.0	36.0
25	35.00187113041248	36.0	36.0	36.0	36.0	36.0
26	34.96247682978984	36.0	36.0	36.0	36.0	36.0
27	34.940047557897984	36.0	36.0	36.0	32.0	36.0
28	34.93553808840752	36.0	36.0	36.0	32.0	36.0
29	34.91273142668609	36.0	36.0	36.0	32.0	36.0
30	34.90339950272676	36.0	36.0	36.0	32.0	36.0
31	34.902539641468366	36.0	36.0	36.0	32.0	36.0
32	34.86188210183537	36.0	36.0	36.0	32.0	36.0
33	34.846159408786065	36.0	36.0	36.0	32.0	36.0
34	34.82899043135996	36.0	36.0	36.0	32.0	36.0
35	34.80387253940984	36.0	36.0	36.0	32.0	36.0
36	34.78044725214443	36.0	36.0	36.0	32.0	36.0
37	34.765735263294374	36.0	36.0	36.0	32.0	36.0
38	34.73731634024518	36.0	36.0	36.0	32.0	36.0
39	34.72517941553163	36.0	36.0	36.0	32.0	36.0
40	34.71405263006249	36.0	36.0	36.0	32.0	36.0
41	34.68044064217286	36.0	36.0	36.0	32.0	36.0
42	34.66006724092443	36.0	36.0	36.0	32.0	36.0
43	34.65053927763745	36.0	36.0	36.0	32.0	36.0
44	34.62403442156817	36.0	36.0	36.0	32.0	36.0
45	34.580256636383204	36.0	36.0	36.0	32.0	36.0
46	34.58572652909263	36.0	36.0	36.0	32.0	36.0
47	34.55420204931717	36.0	36.0	36.0	32.0	36.0
48	34.51722914511599	36.0	36.0	36.0	32.0	36.0
49	34.50267590857449	36.0	36.0	36.0	32.0	36.0
50	33.99903392723269	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	17.0
21	41.0
22	124.0
23	383.0
24	1063.0
25	2479.0
26	5119.0
27	9861.0
28	16564.0
29	25740.0
30	37129.0
31	53907.0
32	79711.0
33	130940.0
34	293032.0
35	1113939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.666617131123594	18.342465389629066	11.49785876493765	26.493058714309687
2	16.41770891067069	20.657476727125008	36.33449888388506	26.590315478319233
3	18.343858507312255	24.17829633421128	28.89592450348636	28.581920654990107
4	12.366889278206422	15.844872091111602	36.35520824564743	35.433030385034535
5	14.361377879645412	36.64195365901473	33.71345377793772	15.283214683402136
6	34.0371404036067	35.82783439922262	16.700074574585884	13.434950622584802
7	29.97927180711538	30.76986960277461	20.99208328790155	18.258775302208466
8	28.05091146988254	32.81410217660149	19.73534125814312	19.39964509537285
9	27.33567864917039	14.184095052521025	18.912880009807616	39.567346288500964
10	15.827266189204503	26.610559118851246	31.916106466868506	25.646068225075748
11	37.25374324949592	21.289870981264404	22.43057128797838	19.025814481261296
12	24.30639082558545	24.08797928649594	28.766652749945905	22.838977137972705
13	29.20714803455038	19.502071406901376	25.589572741607174	25.701207816941075
14	23.28167574643245	19.911042211730383	25.232125817701505	31.575156224135657
15	25.040267155842226	27.269804915446034	22.44017552016804	25.249752408543696
16	25.742223537939257	25.96526770667319	23.816292506495568	24.476216248891983
17	23.85341003913442	25.960861058962642	25.4967506622683	24.688978239634633
18	24.86784294029614	24.844171332722805	26.42644033822716	23.861545388753896
19	25.338393822105893	25.273988970951716	25.69239452151998	23.695222685422415
20	25.221504666809412	24.648527473471134	25.478163648207143	24.65180421151231
21	26.394576885550887	24.511412935092906	24.75959759397035	24.334412585385863
22	25.364438239984903	24.762987322978464	25.178455108406357	24.694119328630272
23	24.18633792321473	24.504972449977487	25.76595164099606	25.542737985811726
24	24.45525642452514	25.43562254915531	25.398279034582583	24.71084199173697
25	24.70010784987794	24.67191660362712	25.689343765412676	24.93863178108226
26	24.301023754655933	25.2635938019935	26.0851511225935	24.35023132075706
27	25.10365508829397	25.16444422850615	25.124049957826124	24.60785072537376
28	24.221704095866055	24.889198233047257	26.05328766991723	24.835810001169456
29	24.31074097781253	25.272463592898063	25.8489435062114	24.56785192307801
30	24.380004440545	25.15297564536203	26.078089187159932	24.388930726933037
31	24.67875255712682	25.00642636124455	25.213482308156877	25.101338773471753
32	24.363733741306053	25.114276239186058	25.341557569180136	25.18043245032776
33	24.263736735566674	24.728525078062635	25.6585537269223	25.349184459448388
34	24.70005135439447	24.844171332722805	26.174470481957318	24.281306830925402
35	25.517484504701272	24.679769475829254	25.567370016604023	24.23537600286545
36	24.28757782959041	25.173427010377658	25.49511229324772	25.043882866784216
37	25.372347607670502	24.906316364538235	25.203369616616	24.51796641117526
38	24.408704146147038	24.807336277501296	25.859338675169614	24.924620901182053
39	24.825979787045924	24.822872535455154	25.11902185979742	25.232125817701505
40	25.004844487707427	24.894508808493303	25.87634381569365	24.224302888105612
41	24.09430678064442	25.069588311762413	26.21650312165794	24.619601785935224
42	25.146252682829275	25.305343964276776	25.657197835319057	23.8912055175749
43	24.41248934353943	24.35944008456244	25.838322355319303	25.389748216578827
44	24.110690470850308	24.88433962146896	25.725839847733372	25.27913005994736
45	24.18554698644617	25.058910665386858	25.90673838579975	24.848803962367228
46	24.034039677930366	24.832264814819	25.917374178837683	25.21632132841295
47	24.29639112501151	24.586325946172234	26.23910131504537	24.878181613770884
48	24.846205170127675	25.42726121760196	25.390878126248197	24.33565548602217
49	23.854765930737667	25.26709652196855	25.84131661594314	25.036820931350647
50	23.647596992858404	25.63250930904329	25.554489046373185	25.165404651725115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	39.5
2	44.0
3	175.0
4	306.0
5	340.0
6	374.0
7	339.0
8	304.0
9	441.0
10	578.0
11	802.0
12	1026.0
13	1778.5
14	2531.0
15	3391.5
16	4252.0
17	5020.5
18	5789.0
19	6174.5
20	6560.0
21	7647.0
22	8734.0
23	9452.0
24	10170.0
25	12451.5
26	14733.0
27	18118.0
28	21503.0
29	24285.5
30	27068.0
31	28861.0
32	30654.0
33	34904.5
34	39155.0
35	45101.0
36	51047.0
37	61340.0
38	71633.0
39	76483.5
40	81334.0
41	88553.5
42	95773.0
43	97240.0
44	98707.0
45	107579.5
46	116452.0
47	122237.5
48	128023.0
49	130391.5
50	132760.0
51	127394.5
52	122029.0
53	119251.5
54	116474.0
55	117182.0
56	117890.0
57	115820.5
58	113751.0
59	103326.5
60	92902.0
61	81916.0
62	70930.0
63	62163.0
64	53396.0
65	45620.5
66	37845.0
67	33324.5
68	28804.0
69	26502.5
70	24201.0
71	19042.5
72	13884.0
73	12392.5
74	10901.0
75	8046.5
76	5192.0
77	4720.5
78	4249.0
79	3727.0
80	3205.0
81	2533.0
82	1861.0
83	1615.0
84	1369.0
85	1181.5
86	994.0
87	708.0
88	422.0
89	286.0
90	150.0
91	90.5
92	31.0
93	22.0
94	13.0
95	11.0
96	9.0
97	5.0
98	1.0
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01581873537120075
2	0.0041806657766744835
3	6.214503181543151E-4
4	2.2598193387429643E-4
5	0.0
6	7.344412850914634E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.649548346857411E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1770053.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.82197919428377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8534259912857	46.51073668657683
2	11.502394279071336	13.071776168196802
3	2.9057625526485524	4.9533353793037485
4	1.1941837220743776	2.7142353043945056
5	0.6350171119614489	1.8041464561943812
6	0.38814761413620885	1.3233189392855127
7	0.25244430414016855	1.004106949829767
8	0.17936710309273282	0.8153595040059374
9	0.14669908525916428	0.7502159133375024
>10	0.7623276646124136	8.053047375858004
>50	0.08516251218822804	3.3817494908564236
>100	0.08285981490127085	10.009662082581162
>500	0.009706555155388555	3.6731236149471664
>1k	0.0025016894730382874	1.935186134632299
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1832	0.10349972571442777	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1810	0.10225682507811913	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1776	0.1003359786401876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.64954834685741E-5	0.0
10	0.0	0.0	0.0	5.64954834685741E-5	0.0
11	0.0	0.0	0.0	5.64954834685741E-5	0.0
12	0.0	0.0	0.0	5.64954834685741E-5	2.259819338742964E-4
13	0.0	0.0	0.0	5.64954834685741E-5	2.259819338742964E-4
14	0.0	0.0	0.0	5.64954834685741E-5	2.259819338742964E-4
15	0.0	0.0	0.0	5.64954834685741E-5	4.519638677485928E-4
16	0.0	0.0	0.0	5.64954834685741E-5	4.519638677485928E-4
17	0.0	0.0	0.0	5.64954834685741E-5	4.519638677485928E-4
18	0.0	0.0	0.0	5.64954834685741E-5	6.214503181543151E-4
19	0.0	0.0	0.0	2.259819338742964E-4	6.214503181543151E-4
20	0.0	0.0	0.0	3.954683842800187E-4	6.214503181543151E-4
21	0.0	0.0	0.0	5.649548346857411E-4	6.214503181543151E-4
22	0.0	0.0	0.0	0.0012429006363086303	6.214503181543151E-4
23	0.0	0.0	0.0	0.002146828371805816	6.779458016228893E-4
24	0.0	0.0	0.0	0.0037287019089258908	6.779458016228893E-4
25	0.0	0.0	0.0	0.004689125127891651	6.779458016228893E-4
26	0.0	0.0	0.0	0.005988521247668855	7.344412850914634E-4
27	0.0	0.0	0.0	0.008474322520286116	7.344412850914634E-4
28	0.0	0.0	0.0	0.01553625795385788	7.344412850914634E-4
29	0.0	0.0	0.0	0.03197644364321294	7.344412850914634E-4
30	0.0	0.0	0.0	0.06276648213358584	7.344412850914634E-4
31	5.64954834685741E-5	0.0	0.0	0.13010909842812615	7.344412850914634E-4
32	5.64954834685741E-5	0.0	0.0	0.20496561402398686	7.344412850914634E-4
33	5.64954834685741E-5	0.0	0.0	0.2829293812106191	7.344412850914634E-4
34	5.64954834685741E-5	0.0	0.0	0.37077985800425184	7.909367685600374E-4
35	5.64954834685741E-5	0.0	0.0	0.47269771018155954	7.909367685600374E-4
36	5.64954834685741E-5	0.0	0.0	0.6200944265510694	7.909367685600374E-4
37	5.64954834685741E-5	0.0	0.0	0.8281672921658279	7.909367685600374E-4
38	5.64954834685741E-5	0.0	0.0	1.1026788463396293	8.474322520286116E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGTCG	60	9.75496E-6	25.667126	5
TTGTACG	60	0.0074126613	18.333662	4
TAGCTCG	215	0.0	17.39517	36
CCTATTC	620	0.0	17.032564	3
GTCCTAT	585	0.0	16.927206	1
GTATCAA	3520	0.0	16.754087	1
CGCAATA	540	0.0	16.703533	36
TCTATAC	185	4.2200554E-10	16.648949	3
GTATTAC	160	2.1245796E-8	16.504026	1
CTATTCC	640	0.0	16.500298	4
TAGGACG	215	1.2732926E-11	16.372387	4
ACCGAGT	135	1.0862586E-6	16.29613	8
CATTCCG	205	1.05501385E-10	16.097397	9
GATTTCG	110	5.6241035E-5	15.999836	41
TCCATTA	615	0.0	15.7396755	8
CAAGACG	700	0.0	15.714568	4
ACCGTCG	470	0.0	15.44665	8
GAACCGT	100	4.9446296E-4	15.400277	6
AAGACGG	705	0.0	15.291056	5
CGGTCCA	610	0.0	15.147385	10
>>END_MODULE