FastQCFastQC Report
Thu 2 Feb 2017
SRR4062613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501136
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG51231.0222773857795089No Hit
TCCATGTACTCTGCGTTGATACCAC43000.858050509243No Hit
GTATCAACGCAGAGTACTTTTTTTT32490.6483270010536062No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27480.5483541393952939No Hit
GAGTACATGGAAGCAGTGGTATCAA22750.45396858337856394No Hit
TATCAACGCAGAGTACTTTTTTTTT21900.4370071198237604No Hit
CATGTACTCTGCGTTGATACCACTG20560.41026787139618787No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19540.3899141151304237No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15320.3057054372465758No Hit
GTACATGGGGTGGTATCAACGCAAA14370.28674850739120716No Hit
GCTTCCATGTACTCTGCGTTGATAC14040.2801634685993423No Hit
CCCATGTACTCTGCGTTGATACCAC13730.27397752306759043No Hit
GTACATGGGAAGCAGTGGTATCAAC13280.2649979247150474No Hit
GCGTTGATACCACTGCTTCCATGTA12340.24624054148973534No Hit
GGTATCAACGCAGAGTACTTTTTTT11780.23506593020657068No Hit
CATGGAAGCAGTGGTATCAACGCAG11270.2248890520736886No Hit
GTATCAACGCAGAGTACATGGAAGC9910.19775071038600298No Hit
GTATCAACGCAGAGTACATGGGAAG9160.18278471313176461No Hit
ACTCTGCGTTGATACCACTGCTTCC8730.17420420803933465No Hit
GTACATGGGTGGTATCAACGCAAAA8170.16302959675617No Hit
CAGTGGTATCAACGCAGAGTACATG8130.16223141023594395No Hit
ACGCAGAGTACATGGAAGCAGTGGT8070.16103413045560488No Hit
GTATCAACGCAGAGTACATGGGGTG7080.14127901408001023No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6860.13688898821876697No Hit
GTACATGGGAGTGGTATCAACGCAA6020.12012707129401999No Hit
GCGTTGATACCACTGCTTCCCATGT5690.1135420325021551No Hit
TATCAACGCAGAGTACATGGAAGCA5260.1049615274097251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG508.688899E-515.20128110
GTATTAT456.7979906E-414.7642681
CCGTCGT602.5593698E-514.2512019
ATCCCCG551.9512678E-413.82210612
CGAGCCG551.9512678E-413.82210615
CCGACCA551.9545798E-413.81934459
AGAATTT1051.3569661E-913.57392717
AAGACGG852.687193E-713.4115535
CGACCAT500.001496121613.3011210
CAGTCTG500.001496121613.301129
ACCGTCG751.47408555E-512.6664688
CAAGACG905.3945587E-712.6639364
TCTGGAC604.0977524E-412.6614073
GATATAC751.4877756E-512.6550881
GTCCAGT751.4877756E-512.6550881
GCATCGC701.0851711E-412.21775514
TGCTACA550.00307299712.0858892
CGGTCCA951.0340391E-612.00100910
TTTAGGA951.0403E-611.9950172
GCAAGAC951.0403E-611.9950173
Produced by FastQC (version 0.11.5)