FastQCFastQC Report
Thu 2 Feb 2017
SRR4062613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501136
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21570.4304220810318955No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20340.40587784553494466No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10900.21750582676159763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.18118834009131254No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9080.18118834009131254No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8730.17420420803933465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.11473931228249416No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5550.11074837968136395No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG5550.11074837968136395No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA5400.10775518023051625No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.10655790045017718No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5180.10336515436927302No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5100.10176878132882092No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5020.10017240828836882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT250.002351482935.29
GTATGCG250.002351482935.211
TCGTAGG250.002351482935.216
TGGACGC454.0648476E-529.33333235
ACCGTCG1001.7389539E-924.1999998
AGAACTA551.5918033E-424.05
CTCTTCG952.6773705E-823.15789438
CGCAATA952.6773705E-823.15789436
CTAGCGG952.6773705E-823.15789429
TCTATAC602.8695283E-422.0000023
AAACGCT904.0719533E-721.99999829
CGTCGTA1351.1277734E-1021.18518610
TAGCGGC1057.723429E-820.95238130
TCTAGCG1057.723429E-820.95238128
CAATACG1057.723429E-820.95238138
AATACGA1057.723429E-820.95238139
AACGCTT956.849805E-720.84210630
CGCGGGA856.101671E-620.70588344
CCCCGCG856.101671E-620.70588341
TACGCTA755.4644435E-520.53333326
Produced by FastQC (version 0.11.5)