FastQCFastQC Report
Thu 2 Feb 2017
SRR4062612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462701
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT97760.6683525888065982No Hit
GTCCTAAAGTGTGTATTTCTCATTT90930.6216581515976266No Hit
CTGTAGGACGTGGAATATGGCAAGA87660.5993022497420867No Hit
CTTTAGGACGTGAAATATGGCGAGG73870.5050246085837092No Hit
GTACATGGAAGCAGTGGTATCAACG69870.477677939647269No Hit
GTCCTACAGTGTGCATTTCTCATTT67410.46085973825135834No Hit
CTGTAGGACCTGGAATATGGCGAGA59130.40425213355292705No Hit
TCCATGTACTCTGCGTTGATACCAC59000.4033633668124928No Hit
CTGAAGGACCTGGAATATGGCGAGA43520.29753175802846926No Hit
GTCCTTCAGTGTGCATTTCTCATTT39780.2719626225728977No Hit
ATTTAGAAATGTCCACTGTAGGACG33060.22602021875967818No Hit
GATATACACTGTTCTACAAATCCCG30900.21125301753400047No Hit
GAGTACATGGAAGCAGTGGTATCAA30560.20892855067440305No Hit
TTGTAGAACAGTGTATATCAATGAG30510.20858671731269754No Hit
TTTCTAAATTTTCCACCTTTTTCAG26760.18294921518478485No Hit
GTGTATATCAATGAGTTACAATGAA26610.18192371509966834No Hit
CATGTACTCTGCGTTGATACCACTG25980.17761661474217902No Hit
GAATATGGCAAGAAAACTGAAAATC23750.16237084681011363No Hit
CTGTAGGACATGGAATATGGCAAGA22450.15348317940577055No Hit
GTATCAACGCAGAGTACTTTTTTTT22050.15074851251212654No Hit
GGAATATGGCGAGAAAACTGAAAAT21450.1466465121716605No Hit
GTGTATATCAATGAGTTACAATGAG21390.1462363121376139No Hit
ATTCCAGGTCCTTCAGTGTGCATTT19870.13584457794176663No Hit
GTTCTACAGTGTGGTTTTTATCATT19780.13522927789069672No Hit
GCTTCCATGTACTCTGCGTTGATAC19570.13379357777153364No Hit
ACCTGGAATATGGCGAGAAAACTGA19110.130648710843843No Hit
GTCCACTGTAGGACGTGGAATATGG18480.12634161048635367No Hit
GACCTGGAATATGGCGAGAAAACTG18020.12319674355866304No Hit
ACAGTGGACATTTCTAAATTTTCCA17710.12107737671608894No Hit
GATATACACTGTTCTACAATGCCGG17510.11971004326926692No Hit
GCCATATTCCACGTCCTACAGTGGA17300.11827434315010382No Hit
TTCCAGGTCCTTCAGTGTGCATTTC16210.11082237586492387No Hit
CCCATGTACTCTGCGTTGATACCAC16140.11034380915853617No Hit
CCATATTCCAGGTCCTTCAGTGTGC16090.11000197579683066No Hit
ATACACACTTTAGGACGTGAAATAT16010.10945504241810186No Hit
CATGGAAGCAGTGGTATCAACGCAG15500.10596834212870573No Hit
TATCAACGCAGAGTACTTTTTTTTT15260.10432754199251931No Hit
GAAATATGGCGAGGAAAACTGAAAA15240.10419080864783711No Hit
GCGTTGATACCACTGCTTCCATGTA15210.10398570863081381No Hit
CCATATTTCACGTCCTAAAGTGTGT15060.1029602085456973No Hit
CACTTTAGGACGTGAAATATGGCGA15020.1026867418563329No Hit
CTGTAGAACATATTAGATGAGTGAG14760.1009092083754643No Hit
ATTCCACGTCCTACAGTGGACATTT14670.10029390832439439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA307.7150326E-419.0027059
TAGACGT250.006036492518.9975014
AACCGCG508.7027875E-515.2021667
CGCGGTC508.7053304E-515.20164410
GCCCCCT653.3632205E-614.61896817
CGGTAGG653.400466E-614.603461
AACGCCG1058.913048E-1114.4747835
CCAACGT2300.014.46056219
GACGTGT400.005273916314.2520297
GAACCGC602.5646052E-514.2520286
CTCGTCT1152.5465852E-1114.04499813
TCCAACG5150.014.02381518
CGGCTCG551.9576581E-413.8201498
AGGCGGC551.9616433E-413.8168365
CAGAACG551.9622131E-413.8163644
TAGGACC19600.013.6665694
TCCGATA707.2511502E-613.5733618
TTCCGAT707.2511502E-613.5733617
ATTCCGA707.2511502E-613.5733616
TCTCGTC1205.0931703E-1113.4602512
Produced by FastQC (version 0.11.5)