##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062612_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1462701
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30545887368642	32.0	32.0	32.0	32.0	32.0
2	31.063038173898835	32.0	32.0	32.0	32.0	32.0
3	31.040851137723976	32.0	32.0	32.0	32.0	32.0
4	31.090555075849405	32.0	32.0	32.0	32.0	32.0
5	30.999389485615993	32.0	32.0	32.0	32.0	32.0
6	34.66615870229117	36.0	36.0	36.0	32.0	36.0
7	34.55263310820188	36.0	36.0	36.0	32.0	36.0
8	34.55383772896853	36.0	36.0	36.0	32.0	36.0
9	34.6544023693154	36.0	36.0	36.0	32.0	36.0
10	34.41842317739579	36.0	36.0	36.0	32.0	36.0
11	34.646998258700854	36.0	36.0	36.0	32.0	36.0
12	34.478056691012036	36.0	36.0	36.0	32.0	36.0
13	34.54860836220116	36.0	36.0	36.0	32.0	36.0
14	34.49224619385643	36.0	36.0	36.0	32.0	36.0
15	34.442501919394324	36.0	36.0	36.0	32.0	36.0
16	34.48535073128411	36.0	36.0	36.0	32.0	36.0
17	34.42447499523143	36.0	36.0	36.0	32.0	36.0
18	34.454524882392235	36.0	36.0	36.0	32.0	36.0
19	34.37124402047992	36.0	36.0	36.0	32.0	36.0
20	34.36163713568255	36.0	36.0	36.0	32.0	36.0
21	34.31073472979098	36.0	36.0	36.0	32.0	36.0
22	34.25492428049205	36.0	36.0	36.0	32.0	36.0
23	34.23780663307129	36.0	36.0	36.0	32.0	36.0
24	34.19276325099935	36.0	36.0	36.0	32.0	36.0
25	33.74976498956383	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	71.0
5	144.0
6	243.0
7	44.0
8	207.0
9	262.0
10	172.0
11	49.0
12	123.0
13	85.0
14	209.0
15	254.0
16	406.0
17	497.0
18	709.0
19	914.0
20	1494.0
21	1982.0
22	2843.0
23	4208.0
24	6052.0
25	8671.0
26	12266.0
27	16714.0
28	23077.0
29	32353.0
30	43573.0
31	61681.0
32	91019.0
33	137153.0
34	324958.0
35	690260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88340347266617	20.43868135775162	12.54173318792669	25.136181981655515
2	11.826186002882451	21.21199690423644	46.96665764191423	19.995159450966877
3	18.912554927079537	26.702269419077123	30.370000157251948	24.015175496591393
4	10.330154683937952	17.731489142311684	40.74275914790498	31.19559702584539
5	10.104346125200305	40.47401332145132	37.234680638756686	12.186959914591702
6	28.907242302680853	39.514375510846186	19.73215415499629	11.846228031476674
7	25.60765949586616	32.43525584672799	23.692717116537764	18.26436754086809
8	22.13593628115121	43.06072176376401	21.08750393452943	13.715838020555351
9	25.888493349045433	15.770269364002043	21.78829174690377	36.55294554004875
10	14.260134131533231	30.07801751458294	36.309151417498434	19.352696936385403
11	33.14160745821184	21.16780976631484	28.70665461962846	16.983928155844865
12	24.14287650658083	24.514601323422497	34.83362747285998	16.508894697136693
13	29.894138970493618	23.351576895381978	26.976834600526225	19.77744953359818
14	18.669515117621813	25.80903285689546	27.81066060834644	27.710791417136292
15	20.12118623708726	38.33929819302053	23.50414802434128	18.035367545550926
16	18.688127621362945	28.57401962394735	34.48502195648127	18.25283079820844
17	18.284531458881368	31.90921804057342	31.59708318188632	18.20916731865889
18	18.888036237603565	27.30559835925857	37.44173581242645	16.36462959071141
19	23.06273694241093	27.4236410509872	27.93267789249438	21.580944114107496
20	22.469680521197965	29.24695162436291	31.07145937347273	17.21190848096639
21	20.4238185011918	28.258833773498885	26.859750796473747	24.457596928835567
22	21.269913434289002	33.28702527680419	27.880211624851338	17.562849664055477
23	18.10109761916661	32.26423916637628	30.188121231704923	19.446541982752187
24	22.247756980299215	28.630761921582966	31.412339107063886	17.709141991053933
25	20.315836094908274	30.3325321856682	31.03035895568314	18.32127276374039
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	480.0
3	774.0
4	774.0
5	774.0
6	1895.5
7	3017.0
8	3017.0
9	3017.0
10	3553.5
11	4090.0
12	4090.0
13	4090.0
14	5745.5
15	7401.0
16	7401.0
17	7401.0
18	13297.5
19	19194.0
20	19194.0
21	19194.0
22	34121.0
23	49048.0
24	49048.0
25	49048.0
26	78547.0
27	108046.0
28	108046.0
29	108046.0
30	145418.5
31	182791.0
32	182791.0
33	182791.0
34	193633.0
35	204475.0
36	204475.0
37	204475.0
38	202004.0
39	199533.0
40	199533.0
41	199533.0
42	196872.5
43	194212.0
44	194212.0
45	194212.0
46	198449.0
47	202686.0
48	202686.0
49	202686.0
50	166144.0
51	129602.0
52	129602.0
53	129602.0
54	94668.0
55	59734.0
56	59734.0
57	59734.0
58	48174.0
59	36614.0
60	36614.0
61	36614.0
62	30723.0
63	24832.0
64	24832.0
65	24832.0
66	20485.5
67	16139.0
68	16139.0
69	16139.0
70	11910.0
71	7681.0
72	7681.0
73	7681.0
74	6398.5
75	5116.0
76	5116.0
77	5116.0
78	4624.0
79	4132.0
80	4132.0
81	4132.0
82	2859.0
83	1586.0
84	1586.0
85	1586.0
86	1196.0
87	806.0
88	806.0
89	806.0
90	580.5
91	355.0
92	355.0
93	355.0
94	207.0
95	59.0
96	59.0
97	59.0
98	325.5
99	592.0
100	592.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009297867438389664
2	0.003896900323442727
3	0.0054693337872880375
4	0.01606616800015861
5	0.03746493644292306
6	0.045532203779172914
7	0.06740953892832507
8	0.0875777072689497
9	0.09619190798392836
10	0.11943657657990253
11	0.12039370999267793
12	0.13283644435875822
13	0.11820597647776271
14	0.12237634349056983
15	0.11239480932876916
16	0.11136930924365268
17	0.12264981017993425
18	0.12975994410340871
19	0.1301017774651142
20	0.12025697664799573
21	0.1290079107076566
22	0.14985974577169223
23	0.13632314464815434
24	0.13310991104812261
25	0.1285293440012689
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1462701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.276446980614764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90137928360562	39.19282662321216
2	10.808320136718942	10.219579477862078
3	2.5833322291821013	3.6639230749872285
4	0.957990751036036	1.811615957970979
5	0.5572206676234043	1.3171706674700316
6	0.32099890928045766	0.9105412749259634
7	0.22711522294665876	0.751604055728975
8	0.1785369202630089	0.6752472995917109
9	0.14804898263209382	0.6299306890146126
>10	1.0412231334774757	9.891283342059051
>50	0.13049493413459795	4.38587967021689
>100	0.12403793694184752	11.704576140369802
>500	0.012461746432418012	4.025518786848361
>1k	0.00767991349904831	6.671405790429659
>5k	0.001159232226271443	4.148897149312434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	9776	0.6683525888065982	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	9093	0.6216581515976266	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8766	0.5993022497420867	No Hit
CTTTAGGACGTGAAATATGGCGAGG	7387	0.5050246085837092	No Hit
GTACATGGAAGCAGTGGTATCAACG	6987	0.477677939647269	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6741	0.46085973825135834	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5913	0.40425213355292705	No Hit
TCCATGTACTCTGCGTTGATACCAC	5900	0.4033633668124928	No Hit
CTGAAGGACCTGGAATATGGCGAGA	4352	0.29753175802846926	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3978	0.2719626225728977	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3306	0.22602021875967818	No Hit
GATATACACTGTTCTACAAATCCCG	3090	0.21125301753400047	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3056	0.20892855067440305	No Hit
TTGTAGAACAGTGTATATCAATGAG	3051	0.20858671731269754	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2676	0.18294921518478485	No Hit
GTGTATATCAATGAGTTACAATGAA	2661	0.18192371509966834	No Hit
CATGTACTCTGCGTTGATACCACTG	2598	0.17761661474217902	No Hit
GAATATGGCAAGAAAACTGAAAATC	2375	0.16237084681011363	No Hit
CTGTAGGACATGGAATATGGCAAGA	2245	0.15348317940577055	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2205	0.15074851251212654	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2145	0.1466465121716605	No Hit
GTGTATATCAATGAGTTACAATGAG	2139	0.1462363121376139	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1987	0.13584457794176663	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1978	0.13522927789069672	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1957	0.13379357777153364	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1911	0.130648710843843	No Hit
GTCCACTGTAGGACGTGGAATATGG	1848	0.12634161048635367	No Hit
GACCTGGAATATGGCGAGAAAACTG	1802	0.12319674355866304	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1771	0.12107737671608894	No Hit
GATATACACTGTTCTACAATGCCGG	1751	0.11971004326926692	No Hit
GCCATATTCCACGTCCTACAGTGGA	1730	0.11827434315010382	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1621	0.11082237586492387	No Hit
CCCATGTACTCTGCGTTGATACCAC	1614	0.11034380915853617	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1609	0.11000197579683066	No Hit
ATACACACTTTAGGACGTGAAATAT	1601	0.10945504241810186	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1550	0.10596834212870573	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1526	0.10432754199251931	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1524	0.10419080864783711	No Hit
GCGTTGATACCACTGCTTCCATGTA	1521	0.10398570863081381	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1506	0.1029602085456973	No Hit
CACTTTAGGACGTGAAATATGGCGA	1502	0.1026867418563329	No Hit
CTGTAGAACATATTAGATGAGTGAG	1476	0.1009092083754643	No Hit
ATTCCACGTCCTACAGTGGACATTT	1467	0.10029390832439439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.836667234110047E-5	0.0
9	0.0	0.0	0.0	2.7346668936440187E-4	0.0
10	0.0	0.0	0.0	3.4183336170550235E-4	0.0
11	0.0	0.0	0.0	3.4183336170550235E-4	0.0
12	0.0	0.0	0.0	3.4183336170550235E-4	0.0
13	0.0	0.0	0.0	4.102000340466028E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	30	7.7150326E-4	19.002705	9
TAGACGT	25	0.0060364925	18.997501	4
AACCGCG	50	8.7027875E-5	15.202166	7
CGCGGTC	50	8.7053304E-5	15.201644	10
GCCCCCT	65	3.3632205E-6	14.618968	17
CGGTAGG	65	3.400466E-6	14.60346	1
AACGCCG	105	8.913048E-11	14.474783	5
CCAACGT	230	0.0	14.460562	19
GACGTGT	40	0.0052739163	14.252029	7
GAACCGC	60	2.5646052E-5	14.252028	6
CTCGTCT	115	2.5465852E-11	14.044998	13
TCCAACG	515	0.0	14.023815	18
CGGCTCG	55	1.9576581E-4	13.820149	8
AGGCGGC	55	1.9616433E-4	13.816836	5
CAGAACG	55	1.9622131E-4	13.816364	4
TAGGACC	1960	0.0	13.666569	4
TCCGATA	70	7.2511502E-6	13.573361	8
TTCCGAT	70	7.2511502E-6	13.573361	7
ATTCCGA	70	7.2511502E-6	13.573361	6
TCTCGTC	120	5.0931703E-11	13.46025	12
>>END_MODULE