FastQCFastQC Report
Thu 2 Feb 2017
SRR4062612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462701
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA47250.32303252681169975No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC37620.25719542134721995No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA36170.2472822538577604No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA33070.22608858543201926No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30010.2051683836956425No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27200.18595734876779327No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA24370.16660958049526184No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA17040.1164968096692352No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA16970.1160182429628475No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG16700.11417234280963778No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA15630.10685710886914003No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA14760.1009092083754643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCC207.857458E-443.999694
CCGGTGT353.218323E-431.4283513
GTCGCGG300.005745600429.33312835
TGAGCGG300.005745600429.33312829
TTCGTCG508.3429986E-526.39981843
TTCGTAA450.00139841124.44427344
TAGGACC23500.024.246644
ATAACGG1407.2759576E-1221.99984635
CGCCCCT1205.984475E-1021.9998465
AGGACCT39700.021.7227695
TAACGGA1451.2732926E-1121.2412336
GGACCTG39300.021.1601586
TGTAGGA60900.020.9536672
GTCCTAC60900.020.4145551
CTGTAGG59700.020.308881
TACGGCG654.9330766E-420.3075516
TCGGAAA654.9330766E-420.3075538
TCTCGGA654.9330766E-420.3075536
CAGGACG654.9330766E-420.307554
ACTGTTC14150.020.2118728
Produced by FastQC (version 0.11.5)