##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1462701
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329611451691083	32.0	32.0	32.0	32.0	32.0
2	31.4878297068232	32.0	32.0	32.0	32.0	32.0
3	31.559547029775736	32.0	32.0	32.0	32.0	32.0
4	31.651708038758432	32.0	32.0	32.0	32.0	32.0
5	31.615261765733393	32.0	32.0	32.0	32.0	32.0
6	35.22587459774759	36.0	36.0	36.0	36.0	36.0
7	35.241127886013615	36.0	36.0	36.0	36.0	36.0
8	35.17989459226458	36.0	36.0	36.0	36.0	36.0
9	35.30651377144064	36.0	36.0	36.0	36.0	36.0
10	35.212637442648905	36.0	36.0	36.0	36.0	36.0
11	35.316308664586956	36.0	36.0	36.0	36.0	36.0
12	35.25500153483179	36.0	36.0	36.0	36.0	36.0
13	35.278843044477306	36.0	36.0	36.0	36.0	36.0
14	35.24929770335837	36.0	36.0	36.0	36.0	36.0
15	35.19886292550562	36.0	36.0	36.0	36.0	36.0
16	35.23695410066719	36.0	36.0	36.0	36.0	36.0
17	35.23051122546576	36.0	36.0	36.0	36.0	36.0
18	35.24254581079797	36.0	36.0	36.0	36.0	36.0
19	35.20259642948217	36.0	36.0	36.0	36.0	36.0
20	35.187633699573595	36.0	36.0	36.0	36.0	36.0
21	35.14756262558103	36.0	36.0	36.0	36.0	36.0
22	35.11276398935941	36.0	36.0	36.0	36.0	36.0
23	35.08741089258844	36.0	36.0	36.0	36.0	36.0
24	35.070067635148945	36.0	36.0	36.0	36.0	36.0
25	35.02486222406356	36.0	36.0	36.0	36.0	36.0
26	34.98839885937044	36.0	36.0	36.0	36.0	36.0
27	34.93465239990948	36.0	36.0	36.0	36.0	36.0
28	34.878074192880156	36.0	36.0	36.0	36.0	36.0
29	34.83739123717014	36.0	36.0	36.0	36.0	36.0
30	34.792010807403564	36.0	36.0	36.0	32.0	36.0
31	34.78090805981537	36.0	36.0	36.0	32.0	36.0
32	34.737304479862935	36.0	36.0	36.0	32.0	36.0
33	34.766308356936925	36.0	36.0	36.0	32.0	36.0
34	34.73291739049881	36.0	36.0	36.0	32.0	36.0
35	34.69099563068597	36.0	36.0	36.0	32.0	36.0
36	34.62289217003338	36.0	36.0	36.0	32.0	36.0
37	34.537325126597985	36.0	36.0	36.0	32.0	36.0
38	34.47709955759926	36.0	36.0	36.0	32.0	36.0
39	34.28616169675142	36.0	36.0	36.0	32.0	36.0
40	34.33473690111649	36.0	36.0	36.0	32.0	36.0
41	34.32112919865372	36.0	36.0	36.0	32.0	36.0
42	34.26909258966802	36.0	36.0	36.0	32.0	36.0
43	34.28465284429285	36.0	36.0	36.0	32.0	36.0
44	34.20747097322009	36.0	36.0	36.0	32.0	36.0
45	34.118022070129165	36.0	36.0	36.0	32.0	36.0
46	34.0648389520483	36.0	36.0	36.0	32.0	36.0
47	34.037809504471525	36.0	36.0	36.0	32.0	36.0
48	33.99935666961327	36.0	36.0	36.0	32.0	36.0
49	33.96719561961057	36.0	36.0	36.0	32.0	36.0
50	33.532659101210704	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	9.0
21	28.0
22	103.0
23	383.0
24	914.0
25	2246.0
26	4947.0
27	9875.0
28	16349.0
29	24893.0
30	35667.0
31	50809.0
32	74264.0
33	113930.0
34	243614.0
35	884669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14766586597798	21.252506853033626	13.016353604911075	25.583473676077322
2	11.42776270980172	21.806456994279486	46.39495539202984	20.370824903888952
3	18.577172379986777	27.081896201048338	30.781442175494107	23.55948924347078
4	10.221870968491766	18.267898272848033	40.94395489975026	30.566275858909947
5	9.883633087008214	40.89154242733136	36.98636973653536	12.238454749125077
6	29.22970375956196	39.680330239277176	19.299143978554685	11.790822022606186
7	25.681360279866194	32.55762121940331	23.682384414018195	18.078634086712302
8	22.255197747181413	43.126517312834274	20.86837979874219	13.749905141242127
9	25.606395291997476	15.663214833380165	21.680234032792757	37.0501558418296
10	14.233667714727755	30.372099287550906	35.93236074905261	19.46187224866873
11	33.042364775849606	21.178901224515467	28.836515460097452	16.94221853953747
12	24.209322342707086	24.57904930672776	34.73717458318549	16.474453767379664
13	30.017344624772935	23.285141666000094	26.93851990256382	19.758993806663153
14	18.693635951571782	25.896475082740768	27.683238064375427	27.726650901312023
15	20.130703404181716	38.39499665345139	23.391793674852206	18.082506267514688
16	18.837000863471072	28.682758814002312	34.032177458004064	18.44806286452255
17	18.380653325594228	32.189080338360334	31.166998586860885	18.263267749184557
18	19.034717279881537	27.407583641496107	37.19775948741404	16.35993959120832
19	23.341407437336816	27.38310837279799	27.97543722196129	21.3000469679039
20	22.75571015539061	29.423785175507504	30.72883658382677	17.09166808527512
21	20.527161737087756	28.23153877655105	26.869811396861014	24.37148808950018
22	21.4394466128074	33.032314875015466	27.982000422506037	17.546238089671096
23	18.129679271430046	32.229553408386266	30.061714595122314	19.579052725061377
24	22.465356897957957	28.672982379857537	31.033273375761688	17.828387346422815
25	20.590537642347957	30.285068513660683	30.615416274412883	18.508977569578473
26	16.574132375652987	32.11462903218087	32.39759868900069	18.913639903165443
27	18.719957120423107	31.863586611344356	30.520933533237482	18.895522734995055
28	17.51526798710058	32.20945360671798	28.42139302564229	21.853885380539154
29	19.54336532209932	29.46083991191638	28.14560186941829	22.85019289656601
30	18.329378321338403	29.56345828710037	31.03949474294473	21.067668648616497
31	21.94002738768894	27.695544065396827	29.646250327305445	20.71817821960879
32	18.90030840205893	31.335385700837016	30.29518678116717	19.469119115936888
33	17.76767774138392	31.261139494674577	32.571113303402406	18.400069460539097
34	19.000465577038643	31.383926038199196	32.39876092243049	17.21684746233167
35	18.805278727504803	32.14908583504079	31.092615647353767	17.953019790100644
36	17.27386526706415	30.989655438808068	32.74175651756579	18.99472277656199
37	18.48532270094845	30.610152040642618	28.710584049645142	22.193941208763786
38	19.30100546865012	33.056995243730604	28.1263224678181	19.515676819801175
39	21.644136429796657	28.327525584517954	29.77327560451521	20.255062381170177
40	23.56353075577305	28.397669790339926	30.486886930411615	17.551912523475405
41	21.947616088318803	26.656233912467414	32.82735159133685	18.568798407876937
42	20.61856797800781	32.13527576722789	30.77648815444852	16.469668100315786
43	17.699721269076864	31.67947516272977	31.70429226478959	18.916511303403773
44	17.328421871592347	31.75283260215177	30.947199735284244	19.971545790971636
45	17.937090355445164	32.22353714122025	29.93783418484024	19.901538318494346
46	18.723332688406842	28.102911125255435	31.262686307982708	21.911069878355015
47	22.583221041074015	28.205422707716753	30.156539169659418	19.054817081549817
48	18.400342927228465	30.693080814192374	29.08933541441484	21.817240844164324
49	20.820659861448103	28.695885215091803	30.262097311754076	20.221357611706015
50	17.90823961971722	33.51860701537772	27.936810052088568	20.636343312816493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	14.0
2	14.0
3	58.5
4	103.0
5	128.0
6	153.0
7	173.0
8	193.0
9	241.5
10	290.0
11	455.0
12	620.0
13	1152.5
14	1685.0
15	2306.5
16	2928.0
17	3732.0
18	4536.0
19	5306.0
20	6076.0
21	7738.5
22	9401.0
23	11482.0
24	13563.0
25	18040.0
26	22517.0
27	32918.0
28	43319.0
29	54089.0
30	64859.0
31	78564.5
32	92270.0
33	114272.5
34	136275.0
35	158030.5
36	179786.0
37	178972.5
38	178159.0
39	160385.0
40	142611.0
41	125786.0
42	108961.0
43	95465.0
44	81969.0
45	78329.0
46	74689.0
47	72984.5
48	71280.0
49	63407.0
50	55534.0
51	45735.5
52	35937.0
53	31016.5
54	26096.0
55	25401.0
56	24706.0
57	23119.0
58	21532.0
59	18773.5
60	16015.0
61	13729.5
62	11444.0
63	9998.5
64	8553.0
65	7426.0
66	6299.0
67	5736.0
68	5173.0
69	5323.0
70	5473.0
71	4232.0
72	2991.0
73	2880.5
74	2770.0
75	1894.0
76	1018.0
77	962.5
78	907.0
79	817.5
80	728.0
81	588.0
82	448.0
83	405.5
84	363.0
85	303.0
86	243.0
87	184.5
88	126.0
89	89.0
90	52.0
91	32.0
92	12.0
93	8.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0143570011916311
2	0.004785667063877033
3	5.469333787288038E-4
4	2.734666893644019E-4
5	0.0
6	2.734666893644019E-4
7	1.3673334468220096E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.3673334468220096E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1462701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.2659800291516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91044928879066	50.56945461075395
2	10.709051275102563	12.90782940552983
3	2.404177164376876	4.346702789246373
4	0.8925806072036031	2.151689801925614
5	0.46337500803208975	1.3962874490034938
6	0.2924543310266707	1.0575028123855337
7	0.19332935178805996	0.8155827997735633
8	0.13200109158940374	0.6364140119642573
9	0.11274613813143819	0.6115280858093899
>10	0.7214281409411174	8.3787740647028
>50	0.08947898968807452	3.7173441626296744
>100	0.07104724445358838	8.263913715088076
>500	0.005596914129234692	2.274045229094253
>1k	0.0022844547466264052	2.872931062093194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4725	0.32303252681169975	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	3762	0.25719542134721995	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	3617	0.2472822538577604	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	3307	0.22608858543201926	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3001	0.2051683836956425	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2720	0.18595734876779327	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2437	0.16660958049526184	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1704	0.1164968096692352	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1697	0.1160182429628475	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1670	0.11417234280963778	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1563	0.10685710886914003	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1476	0.1009092083754643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.836667234110047E-5	0.0	0.0	6.836667234110047E-5	0.0
11	6.836667234110047E-5	0.0	0.0	6.836667234110047E-5	0.0
12	6.836667234110047E-5	0.0	0.0	6.836667234110047E-5	0.0
13	6.836667234110047E-5	0.0	0.0	1.3673334468220093E-4	0.0
14	6.836667234110047E-5	0.0	0.0	1.3673334468220093E-4	0.0
15	6.836667234110047E-5	0.0	0.0	1.3673334468220093E-4	0.0
16	6.836667234110047E-5	0.0	0.0	2.051000170233014E-4	0.0
17	6.836667234110047E-5	0.0	0.0	2.051000170233014E-4	0.0
18	6.836667234110047E-5	0.0	0.0	2.051000170233014E-4	0.0
19	6.836667234110047E-5	0.0	0.0	3.4183336170550235E-4	0.0
20	6.836667234110047E-5	0.0	0.0	8.204000680932057E-4	0.0
21	6.836667234110047E-5	0.0	0.0	0.0012306001021398084	0.0
22	6.836667234110047E-5	0.0	0.0	0.0016408001361864113	0.0
23	6.836667234110047E-5	0.0	0.0	0.0023928335319385167	0.0
24	6.836667234110047E-5	0.0	0.0	0.004033633668124928	0.0
25	6.836667234110047E-5	0.0	0.0	0.005264233770264737	0.0
26	6.836667234110047E-5	0.0	0.0	0.00717850059581555	0.0
27	6.836667234110047E-5	0.0	0.0	0.00868256738731976	0.0
28	6.836667234110047E-5	0.0	0.0	0.012169267676715884	0.0
29	6.836667234110047E-5	0.0	0.0	0.018048801498050524	0.0
30	6.836667234110047E-5	0.0	0.0	0.029534402451355402	0.0
31	6.836667234110047E-5	0.0	0.0	0.05715453807715999	0.0
32	6.836667234110047E-5	0.0	0.0	0.09947350825630119	0.0
33	6.836667234110047E-5	0.0	0.0	0.13748537807795305	0.0
34	6.836667234110047E-5	0.0	0.0	0.1867777488358865	0.0
35	6.836667234110047E-5	0.0	0.0	0.23921498652151055	0.0
36	6.836667234110047E-5	0.0	0.0	0.3159907595605664	0.0
37	6.836667234110047E-5	0.0	0.0	0.4370681362766553	0.0
38	6.836667234110047E-5	0.0	0.0	0.5798177481248731	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCC	20	7.857458E-4	43.99969	4
CCGGTGT	35	3.218323E-4	31.42835	13
GTCGCGG	30	0.0057456004	29.333128	35
TGAGCGG	30	0.0057456004	29.333128	29
TTCGTCG	50	8.3429986E-5	26.399818	43
TTCGTAA	45	0.001398411	24.444273	44
TAGGACC	2350	0.0	24.24664	4
ATAACGG	140	7.2759576E-12	21.999846	35
CGCCCCT	120	5.984475E-10	21.999846	5
AGGACCT	3970	0.0	21.722769	5
TAACGGA	145	1.2732926E-11	21.24123	36
GGACCTG	3930	0.0	21.160158	6
TGTAGGA	6090	0.0	20.953667	2
GTCCTAC	6090	0.0	20.414555	1
CTGTAGG	5970	0.0	20.30888	1
TACGGCG	65	4.9330766E-4	20.30755	16
TCGGAAA	65	4.9330766E-4	20.30755	38
TCTCGGA	65	4.9330766E-4	20.30755	36
CAGGACG	65	4.9330766E-4	20.30755	4
ACTGTTC	1415	0.0	20.211872	8
>>END_MODULE