FastQCFastQC Report
Thu 2 Feb 2017
SRR4062611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33357
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1270.3807296819258327No Hit
TATCAACGCAGAGTACTTTTTTTTT730.21884462031957314No Hit
CTCCGAGGTCACCCCAACCGAAATT590.1768744191623947No Hit
GGTATCAACGCAGAGTACTTTTTTT500.14989357556135144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT490.14689570405012442No Hit
GAGTACTTTTTTTTTTTTTTTTTTT470.14089996102767036No Hit
GTATCTGATCGTCTTCGAACCTCCG450.1349042180052163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG450.1349042180052163No Hit
GGACTCTTTCGAGGCCCTGTAATTG370.11092124591540006No Hit
GTACTTTTTTTTTTTTTTTTTTTTT360.10792337440417303No Hit
CTCTTAATCATGGCCTCAGTTCCGA350.104925502892946No Hit
GATTAAGAGGGACGGCCGGGGGCAT350.104925502892946No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT340.10192763138171898No Hit
GTCTTGCGCCGGTCCAAGAATTTCA340.10192763138171898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG1106.703142E-48.6349995
TCAACGC1050.00428709068.141573
CAACGCA1100.00651448837.7714994
Produced by FastQC (version 0.11.5)