##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062611_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33357
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.182060736876817	32.0	32.0	32.0	14.0	32.0
2	27.92739155199808	32.0	32.0	32.0	14.0	32.0
3	28.208531942320953	32.0	32.0	32.0	14.0	32.0
4	27.795365290643645	32.0	21.0	32.0	14.0	32.0
5	27.96699343466139	32.0	21.0	32.0	14.0	32.0
6	31.186077884701863	36.0	32.0	36.0	14.0	36.0
7	31.24390682615343	36.0	32.0	36.0	14.0	36.0
8	30.98042389903169	36.0	32.0	36.0	14.0	36.0
9	31.25826663069221	36.0	32.0	36.0	14.0	36.0
10	30.26039511946518	36.0	21.0	36.0	14.0	36.0
11	31.689360554006655	36.0	32.0	36.0	14.0	36.0
12	30.76283838474683	36.0	32.0	36.0	14.0	36.0
13	31.166591719878888	36.0	32.0	36.0	14.0	36.0
14	30.774440147495277	36.0	32.0	36.0	14.0	36.0
15	30.640195461222532	36.0	27.0	36.0	14.0	36.0
16	30.706778187486883	36.0	27.0	36.0	14.0	36.0
17	30.549120124711454	36.0	27.0	36.0	14.0	36.0
18	30.72260694906616	36.0	32.0	36.0	14.0	36.0
19	30.63357016518272	36.0	27.0	36.0	14.0	36.0
20	30.50430194561861	36.0	27.0	36.0	14.0	36.0
21	30.53859759570705	36.0	27.0	36.0	14.0	36.0
22	30.446473004167043	36.0	27.0	36.0	14.0	36.0
23	30.34649398926762	36.0	27.0	36.0	14.0	36.0
24	30.37962046946668	36.0	27.0	36.0	14.0	36.0
25	29.758581407200886	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	2.0
6	10.0
7	1.0
8	3.0
9	12.0
10	3.0
11	2.0
12	2.0
13	2.0
14	19.0
15	85.0
16	196.0
17	380.0
18	613.0
19	791.0
20	905.0
21	891.0
22	879.0
23	912.0
24	870.0
25	964.0
26	971.0
27	1093.0
28	1255.0
29	1358.0
30	1579.0
31	1908.0
32	2471.0
33	3115.0
34	5382.0
35	6682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.932094138809774	19.42437415679808	11.350622095637835	26.29290960875431
2	17.082383978894352	20.521045688931526	37.63340928168845	24.763161050485667
3	19.055042571051683	24.05264420194268	29.11320302194508	27.779110205060558
4	12.921213574769158	15.640364552104568	36.002518287564456	35.43590358556182
5	15.331794056793308	35.42535008545983	33.575219646766016	15.66763621098084
6	32.92046545105566	36.2943857965451	16.935580614203456	13.849568138195778
7	29.577845119865582	30.474961745026853	21.55180173422545	18.395391400882115
8	27.0741440873766	32.313139496504334	20.676928616437124	19.93578779968194
9	26.568033131264627	14.191825220574996	19.94478122561671	39.29536042254366
10	15.754246953598654	25.967945254817216	31.811633351341616	26.46617444024251
11	36.07659523380755	21.71198751425656	22.957560477819797	19.253856774116095
12	24.387608069164266	24.11743515850144	28.491234390009605	23.00372238232469
13	29.108263048893956	19.91475823153345	25.299396704385146	25.677582015187443
14	23.508224276623842	20.233521431144194	25.546284067715213	30.711970224516747
15	24.41849995498064	27.503826645457547	23.052912752483568	25.024760647078242
16	24.694618686034993	26.63345238452535	24.313454785557788	24.35847414388187
17	23.426478974697602	26.43395263679203	26.139808506168023	23.999759882342346
18	24.271349242083147	24.05523037670719	27.645204862674472	24.028215518535195
19	24.934714092751012	25.342938616238932	26.174395917754765	23.54795137325529
20	24.93097238895558	24.603841536614645	25.888355342136855	24.57683073229292
21	25.42927473583093	24.756844380403457	25.453290105667627	24.360590778097983
22	25.141090297790587	25.267170989433236	25.534341978866475	24.0573967339097
23	23.54936511271876	24.50394740791883	26.57821270975295	25.368474769609463
24	23.52676293116388	25.50809042058179	26.633844675932878	24.331301972321455
25	23.870561042235764	25.1373337736019	26.60522918980578	24.386875994356558
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	15.0
3	26.0
4	26.0
5	26.0
6	58.0
7	90.0
8	90.0
9	90.0
10	107.0
11	124.0
12	124.0
13	124.0
14	137.0
15	150.0
16	150.0
17	150.0
18	253.5
19	357.0
20	357.0
21	357.0
22	545.0
23	733.0
24	733.0
25	733.0
26	961.5
27	1190.0
28	1190.0
29	1190.0
30	1489.0
31	1788.0
32	1788.0
33	1788.0
34	2098.5
35	2409.0
36	2409.0
37	2409.0
38	2574.0
39	2739.0
40	2739.0
41	2739.0
42	3002.5
43	3266.0
44	3266.0
45	3266.0
46	3711.0
47	4156.0
48	4156.0
49	4156.0
50	4135.5
51	4115.0
52	4115.0
53	4115.0
54	3829.5
55	3544.0
56	3544.0
57	3544.0
58	3237.0
59	2930.0
60	2930.0
61	2930.0
62	2579.5
63	2229.0
64	2229.0
65	2229.0
66	1890.0
67	1551.0
68	1551.0
69	1551.0
70	1194.5
71	838.0
72	838.0
73	838.0
74	666.5
75	495.0
76	495.0
77	495.0
78	398.0
79	301.0
80	301.0
81	301.0
82	225.0
83	149.0
84	149.0
85	149.0
86	120.5
87	92.0
88	92.0
89	92.0
90	58.0
91	24.0
92	24.0
93	24.0
94	15.0
95	6.0
96	6.0
97	6.0
98	28.5
99	51.0
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005995743022454058
2	0.002997871511227029
3	0.002997871511227029
4	0.002997871511227029
5	0.023982972089816233
6	0.038972329645951376
7	0.08394040231435682
8	0.08993614533681087
9	0.104925502892946
10	0.11691698893785411
11	0.11691698893785411
12	0.1349042180052163
13	0.11991486044908115
14	0.12291273196030819
15	0.11391911742662711
16	0.11391911742662711
17	0.11991486044908115
18	0.1259106034715352
19	0.1259106034715352
20	0.11092124591540006
21	0.1349042180052163
22	0.1349042180052163
23	0.13190634649398927
24	0.1379020895164433
25	0.13190634649398927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	33357.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.28770572893245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.16197822141561	73.07311808615883
2	4.151542649727768	6.583325838654555
3	1.1947973381730188	2.8419821926432234
4	0.66167574107683	2.09851005785892
5	0.42725347852389595	1.6937974038432713
6	0.26845130066545675	1.2770932637827141
7	0.24576527525710828	1.364031537608298
8	0.12477313974591651	0.7914380789639356
9	0.09452510586811858	0.6745210900260814
>10	0.6578947368421052	8.825733729052372
>50	0.0075620084694494865	0.3957190394819678
>100	0.0037810042347247433	0.3807296819258327
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	127	0.3807296819258327	No Hit
TATCAACGCAGAGTACTTTTTTTTT	73	0.21884462031957314	No Hit
CTCCGAGGTCACCCCAACCGAAATT	59	0.1768744191623947	No Hit
GGTATCAACGCAGAGTACTTTTTTT	50	0.14989357556135144	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	49	0.14689570405012442	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	47	0.14089996102767036	No Hit
GTATCTGATCGTCTTCGAACCTCCG	45	0.1349042180052163	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	45	0.1349042180052163	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	37	0.11092124591540006	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	36	0.10792337440417303	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	35	0.104925502892946	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	35	0.104925502892946	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	34	0.10192763138171898	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	34	0.10192763138171898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0029978715112270286	0.0
5	0.0	0.0	0.0	0.0029978715112270286	0.0
6	0.0	0.0	0.0	0.0029978715112270286	0.0
7	0.0	0.0	0.0	0.0029978715112270286	0.0
8	0.0	0.0	0.0	0.0029978715112270286	0.0
9	0.0	0.0	0.0	0.0029978715112270286	0.0
10	0.0	0.0	0.0	0.0029978715112270286	0.0
11	0.0	0.0	0.0	0.0029978715112270286	0.0
12	0.0	0.0	0.0	0.0029978715112270286	0.0
13	0.0	0.0	0.0	0.0029978715112270286	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	110	6.703142E-4	8.634999	5
TCAACGC	105	0.0042870906	8.14157	3
CAACGCA	110	0.0065144883	7.771499	4
>>END_MODULE