FastQCFastQC Report
Thu 2 Feb 2017
SRR4062611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33357
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC570.17087867613994062No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT440.13190634649398927No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT400.11991486044908115No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG380.11391911742662711No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG370.11092124591540006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAA250.002320243335.234
AGAACCG250.002320243335.25
CGGCGCA250.002320243335.233
CATGGGG550.004394400420.017
AACGCAG851.3787537E-418.1176476
GTATCAA851.3787537E-418.1176471
TCAACGC851.3787537E-418.1176474
AGTACAT851.3787537E-418.11764713
GAGTACA851.3787537E-418.11764712
CGCAGAG851.3787537E-418.1176478
TATCAAC851.3787537E-418.1176472
ATCAACG851.3787537E-418.1176473
CAACGCA851.3787537E-418.1176475
AGAGTAC902.1352139E-417.1111111
GCAGAGT902.1352139E-417.111119
ACGCAGA953.2259544E-416.2105267
CAGAGTA953.2259544E-416.21052610
ACATGGG1056.9043966E-414.6666673
GTACATG1109.820351E-414.014
TACATGG1150.001373865513.3913052
Produced by FastQC (version 0.11.5)