##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062611_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33357
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.118266031117905	32.0	32.0	32.0	21.0	32.0
2	30.035584734838263	32.0	32.0	32.0	21.0	32.0
3	30.76124951284588	32.0	32.0	32.0	32.0	32.0
4	31.1812812902839	32.0	32.0	32.0	32.0	32.0
5	30.957010522529004	32.0	32.0	32.0	32.0	32.0
6	34.47962946308121	36.0	36.0	36.0	32.0	36.0
7	34.557484186227775	36.0	36.0	36.0	32.0	36.0
8	34.360943729951735	36.0	36.0	36.0	32.0	36.0
9	34.70818718709716	36.0	36.0	36.0	32.0	36.0
10	34.39559912462152	36.0	36.0	36.0	32.0	36.0
11	34.78061576280841	36.0	36.0	36.0	32.0	36.0
12	34.537758191683906	36.0	36.0	36.0	32.0	36.0
13	34.67826842941511	36.0	36.0	36.0	32.0	36.0
14	34.61819108433013	36.0	36.0	36.0	32.0	36.0
15	34.541505531072936	36.0	36.0	36.0	32.0	36.0
16	34.52411787630782	36.0	36.0	36.0	32.0	36.0
17	34.54477321102018	36.0	36.0	36.0	32.0	36.0
18	34.56734718349971	36.0	36.0	36.0	32.0	36.0
19	34.55472614443745	36.0	36.0	36.0	32.0	36.0
20	34.51653326138442	36.0	36.0	36.0	32.0	36.0
21	34.58632371016578	36.0	36.0	36.0	32.0	36.0
22	34.52513715262164	36.0	36.0	36.0	32.0	36.0
23	34.4771412297269	36.0	36.0	36.0	32.0	36.0
24	34.423029648949246	36.0	36.0	36.0	32.0	36.0
25	34.36274245285847	36.0	36.0	36.0	32.0	36.0
26	34.31213838174896	36.0	36.0	36.0	32.0	36.0
27	34.3217615493	36.0	36.0	36.0	32.0	36.0
28	34.32113199628264	36.0	36.0	36.0	32.0	36.0
29	34.22924723446353	36.0	36.0	36.0	32.0	36.0
30	34.22798812842881	36.0	36.0	36.0	32.0	36.0
31	34.169169889378544	36.0	36.0	36.0	32.0	36.0
32	34.14383787510867	36.0	36.0	36.0	32.0	36.0
33	34.113079713403486	36.0	36.0	36.0	32.0	36.0
34	34.09812033456246	36.0	36.0	36.0	32.0	36.0
35	34.04928500764457	36.0	36.0	36.0	32.0	36.0
36	33.97220973109093	36.0	36.0	36.0	32.0	36.0
37	34.01283089006805	36.0	36.0	36.0	32.0	36.0
38	33.91941721377822	36.0	36.0	36.0	32.0	36.0
39	33.955301735767605	36.0	36.0	36.0	32.0	36.0
40	33.951224630512336	36.0	36.0	36.0	32.0	36.0
41	33.88005516083581	36.0	36.0	36.0	32.0	36.0
42	33.81173366909494	36.0	36.0	36.0	32.0	36.0
43	33.8204274964775	36.0	36.0	36.0	32.0	36.0
44	33.74209311388914	36.0	36.0	36.0	32.0	36.0
45	33.65827262643523	36.0	36.0	36.0	27.0	36.0
46	33.683964385286444	36.0	36.0	36.0	27.0	36.0
47	33.74239290104026	36.0	36.0	36.0	32.0	36.0
48	33.684863746739815	36.0	36.0	36.0	27.0	36.0
49	33.63533890937435	36.0	36.0	36.0	27.0	36.0
50	32.925772701382016	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	5.0
22	6.0
23	20.0
24	54.0
25	125.0
26	204.0
27	360.0
28	528.0
29	862.0
30	1289.0
31	1872.0
32	2796.0
33	4328.0
34	7584.0
35	13323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.950524737631184	19.550224887556222	12.308845577211395	25.1904047976012
2	18.758244393812205	20.724907063197026	35.5288403885358	24.98800815445497
3	20.547411337950056	23.99496357586114	28.75858140720089	26.699043678987916
4	14.623617231765445	16.803069820427496	36.343196330605274	32.23011661720179
5	17.681446173217015	33.926911892556284	32.61384417063884	15.777797763587852
6	34.1727373564769	36.0134304643703	15.783793506610309	14.030038672542494
7	29.034385586233775	30.323470336061398	21.26989837215577	19.37224570554906
8	27.439517942260995	32.94660790838505	19.04847558233654	20.565398567017418
9	26.82795215397068	14.48871301376023	18.742692688191383	39.94064214407771
10	16.12255298737896	26.285337410438586	31.051953113289564	26.540156488892887
11	37.42842581766945	21.051053751836196	21.971400305782897	19.549120124711454
12	24.279761369427707	24.222801810714394	28.060077345084988	23.43735947477291
13	28.956440926941873	19.872890247923973	25.050214347813053	26.120454477321104
14	23.66819558113739	20.388524147855023	24.324729442096114	31.618550828911474
15	25.023233504212012	26.902898941751356	22.154270467967745	25.91959708606889
16	25.48490571694097	26.447222472044846	23.404382888149414	24.663488922864765
17	23.518302005576043	26.32430974008454	25.302035554756124	24.855352699583296
18	25.00224840363342	25.170129208262132	26.108462991276195	23.719159396828253
19	25.173127079773362	25.880624756422936	25.82666306922085	23.119585094582845
20	25.320022783823486	25.089186677459	24.615522978685135	24.97526756003238
21	26.192403393590553	25.352999370446984	23.797104056120155	24.657493179842312
22	24.72044848157808	25.547861018676738	25.041220733279374	24.69046976646581
23	24.17483586653476	25.472914230896066	25.541865275654285	24.810384626914892
24	23.71016578229457	26.25835656683754	25.50589081751956	24.525586833348324
25	24.576550649039184	25.604820577390054	24.921305872830292	24.897322900740473
26	23.958989117726414	26.357286326708035	25.221093023952996	24.462631531612555
27	24.693467637977037	25.955571544203615	24.66948466588722	24.681476151932127
28	23.464340318373957	25.91959708606889	25.913601343046437	24.70246125251072
29	24.168840123512307	25.74572053841772	25.523878046586923	24.56156129148305
30	23.803099799142608	25.88961837095662	25.619809934946186	24.687471894954584
31	24.47762088916869	25.952573672692385	24.867344185628205	24.70246125251072
32	23.97397847528255	26.147435320922146	24.753425068201576	25.125161135593725
33	23.491321161974998	25.84465029828821	25.685763108193182	24.978265431543605
34	23.92301465959169	25.805677968642264	26.024522588961833	24.24678478280421
35	25.481907845429745	25.029229247234465	25.35599724195821	24.132865665377583
36	23.73115088287316	25.673771622148273	25.535869532631832	25.059207962346736
37	24.909314386785383	25.08319093443655	25.448931258806244	24.558563419971822
38	23.908025302035554	25.454927001828704	25.562850376232877	25.07419731990287
39	24.189825224090896	25.59582696285637	24.91231225829661	25.302035554756124
40	24.72944209611176	25.793686482597355	25.673771622148273	23.803099799142608
41	23.917018916569237	25.448931258806244	25.883622627934166	24.75042719669035
42	24.7114548670444	25.676769493659503	25.66777587912582	23.94399976017028
43	23.7581317264742	24.777408040291395	26.225379980214047	25.239080253020358
44	23.52129987708727	25.24207812453158	25.793686482597355	25.442935515783795
45	23.584255178823035	26.114458734298644	26.027520460473063	24.273765626405254
46	23.96498486074887	25.57783973378901	25.75771202446263	24.69946338099949
47	23.66219983811494	25.134154750127408	26.249362952303862	24.95428245945379
48	24.027940162484636	26.381269298797854	25.57484186227778	24.015948676439727
49	23.32044248583506	25.66477800761459	26.165422549989508	24.849356956560843
50	23.200527625385973	26.01253110291693	25.350001498935754	25.43693977276134
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	4.0
4	5.0
5	10.5
6	16.0
7	16.0
8	16.0
9	19.0
10	22.0
11	30.5
12	39.0
13	48.5
14	58.0
15	58.5
16	59.0
17	90.5
18	122.0
19	148.5
20	175.0
21	193.0
22	211.0
23	239.5
24	268.0
25	321.0
26	374.0
27	456.5
28	539.0
29	593.5
30	648.0
31	699.0
32	750.0
33	821.0
34	892.0
35	987.0
36	1082.0
37	1242.5
38	1403.0
39	1473.0
40	1543.0
41	1651.0
42	1759.0
43	1734.0
44	1709.0
45	1854.5
46	2000.0
47	2080.0
48	2160.0
49	2175.5
50	2191.0
51	2101.0
52	2011.0
53	1980.0
54	1949.0
55	1996.0
56	2043.0
57	2062.5
58	2082.0
59	1885.5
60	1689.0
61	1525.0
62	1361.0
63	1159.0
64	957.0
65	857.5
66	758.0
67	688.0
68	618.0
69	600.0
70	582.0
71	450.0
72	318.0
73	299.0
74	280.0
75	228.0
76	176.0
77	161.0
78	146.0
79	131.0
80	116.0
81	88.5
82	61.0
83	64.5
84	68.0
85	51.0
86	34.0
87	27.0
88	20.0
89	14.5
90	9.0
91	7.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	12.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020985100578589204
2	0.002997871511227029
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	33357.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.51899151602362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.66154719256554	79.16179512546093
2	3.3838186783953463	5.719938843421171
3	1.0179831873160006	2.5811673711664715
4	0.5533288404923208	1.8706718230056658
5	0.3050402582201256	1.2890847498276226
6	0.23055368353846697	1.1691698893785412
7	0.18444294683077359	1.0912252300866385
8	0.1773489873372823	1.1991486044908115
9	0.0851275139218955	0.6475402464250382
>10	0.39726173163551237	5.0993794405971755
>50	0.003546979746745646	0.17087867613994062
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	57	0.17087867613994062	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	44	0.13190634649398927	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	40	0.11991486044908115	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	38	0.11391911742662711	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	37	0.11092124591540006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0029978715112270286	0.0
28	0.0	0.0	0.0	0.0029978715112270286	0.0
29	0.0	0.0	0.0	0.017987229067362172	0.0
30	0.0	0.0	0.0	0.035974458134724344	0.0
31	0.0	0.0	0.0	0.09593188835926492	0.0
32	0.0	0.0	0.0	0.20385526276343796	0.0
33	0.0	0.0	0.0	0.2907935365890218	0.0
34	0.0	0.0	0.0	0.35374883832478937	0.0
35	0.0	0.0	0.0	0.47965944179632464	0.0
36	0.0	0.0	0.0	0.6475402464250383	0.0
37	0.0	0.0	0.0	0.9053571963905627	0.0
38	0.0	0.0	0.0	1.2591060347153522	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCAA	25	0.0023202433	35.2	34
AGAACCG	25	0.0023202433	35.2	5
CGGCGCA	25	0.0023202433	35.2	33
CATGGGG	55	0.0043944004	20.0	17
AACGCAG	85	1.3787537E-4	18.117647	6
GTATCAA	85	1.3787537E-4	18.117647	1
TCAACGC	85	1.3787537E-4	18.117647	4
AGTACAT	85	1.3787537E-4	18.117647	13
GAGTACA	85	1.3787537E-4	18.117647	12
CGCAGAG	85	1.3787537E-4	18.117647	8
TATCAAC	85	1.3787537E-4	18.117647	2
ATCAACG	85	1.3787537E-4	18.117647	3
CAACGCA	85	1.3787537E-4	18.117647	5
AGAGTAC	90	2.1352139E-4	17.11111	11
GCAGAGT	90	2.1352139E-4	17.11111	9
ACGCAGA	95	3.2259544E-4	16.210526	7
CAGAGTA	95	3.2259544E-4	16.210526	10
ACATGGG	105	6.9043966E-4	14.666667	3
GTACATG	110	9.820351E-4	14.0	14
TACATGG	115	0.0013738655	13.391305	2
>>END_MODULE