FastQCFastQC Report
Thu 2 Feb 2017
SRR4062610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895258
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34820.1837216885511102No Hit
GCGCAAGACGGACCAGAGCGAAAGC23130.12204143182616826No Hit
TATCAACGCAGAGTACTTTTTTTTT22410.11824247674986729No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21540.11365207269933698No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21540.11365207269933698No Hit
GAACTACGACGGTATCTGATCGTCT20140.10626521560652956No Hit
GAATAGGACCGCGGTTCTATTTTGT19670.10378534215394421No Hit
GATTAAGAGGGACGGCCGGGGGCAT19660.10373257888899559No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19530.10304665644466347No Hit
GAATAACGCCGCCGCATCGCCAGTC19350.10209691767558823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG3650.014.83931113
GCGTTAT1600.014.2396241
CGGTCCA4300.013.92096610
CGAACGA1051.36788E-913.57409116
CGCCAGT4000.013.54087218
GAAAGCG1001.0051735E-813.3022589
ATCGCCA4250.012.96724916
AACGGAC1051.9881554E-812.66948615
GCATCGC4350.012.66915214
GTGCGCT550.003066186612.09232111
AAAGCGT951.03803E-612.00203610
CCGGTCC5150.011.9923279
GGTCCAA5100.011.92296111
CGCAGTT802.8598364E-511.87795717
CAAGACG5050.011.8487994
GTTATTC1850.011.8047043
CGTTATT1850.011.8022112
AGGCCCG3550.011.77664610
CGTTCAA1052.7139686E-711.76421214
CGCAAGA4850.011.74401952
Produced by FastQC (version 0.11.5)