##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062610_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1895258
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16897382836532	32.0	32.0	32.0	32.0	32.0
2	30.788522195922667	32.0	32.0	32.0	32.0	32.0
3	30.778242329012727	32.0	32.0	32.0	32.0	32.0
4	30.86393039892194	32.0	32.0	32.0	32.0	32.0
5	30.639885440399144	32.0	32.0	32.0	32.0	32.0
6	34.29646412256273	36.0	36.0	36.0	32.0	36.0
7	34.168911567712684	36.0	36.0	36.0	32.0	36.0
8	34.16516326537073	36.0	36.0	36.0	32.0	36.0
9	34.34526275578312	36.0	36.0	36.0	32.0	36.0
10	33.98393727925169	36.0	36.0	36.0	32.0	36.0
11	34.32665684566428	36.0	36.0	36.0	32.0	36.0
12	34.08684358541159	36.0	36.0	36.0	32.0	36.0
13	34.22388666872795	36.0	36.0	36.0	32.0	36.0
14	34.11730645642968	36.0	36.0	36.0	32.0	36.0
15	34.054803620404186	36.0	36.0	36.0	32.0	36.0
16	34.05793459254624	36.0	36.0	36.0	32.0	36.0
17	33.97543236857462	36.0	36.0	36.0	32.0	36.0
18	33.99818125025722	36.0	36.0	36.0	32.0	36.0
19	33.98314266448156	36.0	36.0	36.0	32.0	36.0
20	33.979461899118746	36.0	36.0	36.0	32.0	36.0
21	33.94456427568173	36.0	36.0	36.0	32.0	36.0
22	33.904082715915195	36.0	36.0	36.0	32.0	36.0
23	33.87653290475492	36.0	36.0	36.0	32.0	36.0
24	33.8428636101259	36.0	36.0	36.0	32.0	36.0
25	33.38599494105816	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	68.0
5	211.0
6	320.0
7	78.0
8	285.0
9	339.0
10	229.0
11	66.0
12	147.0
13	108.0
14	368.0
15	461.0
16	655.0
17	895.0
18	1184.0
19	1658.0
20	2499.0
21	3745.0
22	5617.0
23	8409.0
24	11705.0
25	16444.0
26	22681.0
27	30206.0
28	40444.0
29	53889.0
30	69830.0
31	94655.0
32	136788.0
33	197211.0
34	432638.0
35	761411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47587826339327	17.52584990039972	10.783742068255874	26.214529767951138
2	17.05857925815471	20.115734914597088	36.61758987079416	26.20809595645404
3	19.312360831161158	23.66107704809042	28.333720279867876	28.69284184088055
4	12.707754935325847	15.089175977764185	35.88918188817606	36.31388719873391
5	14.708217211384172	36.39821383329111	33.65050460265181	15.243064352672917
6	34.32732685967973	35.33332101597122	16.733435567438878	13.605916556910172
7	30.114336854165728	30.485146240822825	20.84763973991072	18.55287716510073
8	28.13210966925581	32.92538662869914	19.635889874457895	19.306613827587153
9	27.30735937137724	14.146758571444416	18.79900222297454	39.74687983420381
10	15.977531686861488	26.63270604978296	31.675715024535428	25.714047238820125
11	37.57020108771735	21.104991930605852	22.272214606263752	19.052592375413045
12	24.528766434264	23.816289179644652	28.80764540664831	22.847298979443035
13	29.385397241313733	19.361783055897472	25.428568409819697	25.8242512929691
14	23.11283549224372	19.970172010187795	24.97975278587402	31.937239711694467
15	24.898852686511738	27.692996047080054	22.260015815905813	25.148135450502394
16	25.441828451655198	25.805993241769183	24.21886018639279	24.53331812018283
17	23.78998941293566	26.022412455966474	25.460463337270905	24.72713479382696
18	24.707807954724366	24.727091942885583	26.747314242361163	23.817785860028888
19	25.36475419147013	25.1786192624027	25.407338566385924	24.049287979741248
20	25.329605107802806	24.620393492263652	25.34481947989035	24.70518192004319
21	26.40951796770531	24.45591272413733	24.418401305608697	24.716168002548663
22	25.54678219182136	24.803682628528428	25.030279906824553	24.61925527282566
23	24.089294404278625	24.59448020708338	25.748994357411163	25.567231031226832
24	24.515333442877424	25.435028290377787	25.413577373615265	24.636060893129525
25	24.506308087422976	25.00490033174321	25.494035332052288	24.99475624878152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	328.0
1	328.0
2	713.0
3	1098.0
4	1098.0
5	1098.0
6	2697.5
7	4297.0
8	4297.0
9	4297.0
10	4749.5
11	5202.0
12	5202.0
13	5202.0
14	5860.0
15	6518.0
16	6518.0
17	6518.0
18	10276.5
19	14035.0
20	14035.0
21	14035.0
22	22227.0
23	30419.0
24	30419.0
25	30419.0
26	44812.0
27	59205.0
28	59205.0
29	59205.0
30	73490.5
31	87776.0
32	87776.0
33	87776.0
34	105749.5
35	123723.0
36	123723.0
37	123723.0
38	138301.0
39	152879.0
40	152879.0
41	152879.0
42	172614.5
43	192350.0
44	192350.0
45	192350.0
46	217986.5
47	243623.0
48	243623.0
49	243623.0
50	244855.5
51	246088.0
52	246088.0
53	246088.0
54	228667.5
55	211247.0
56	211247.0
57	211247.0
58	194296.5
59	177346.0
60	177346.0
61	177346.0
62	158350.5
63	139355.0
64	139355.0
65	139355.0
66	115988.0
67	92621.0
68	92621.0
69	92621.0
70	70616.0
71	48611.0
72	48611.0
73	48611.0
74	38261.5
75	27912.0
76	27912.0
77	27912.0
78	22836.0
79	17760.0
80	17760.0
81	17760.0
82	12442.5
83	7125.0
84	7125.0
85	7125.0
86	5276.0
87	3427.0
88	3427.0
89	3427.0
90	2344.0
91	1261.0
92	1261.0
93	1261.0
94	760.0
95	259.0
96	259.0
97	259.0
98	526.0
99	793.0
100	793.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009655677485598266
2	0.0034296122216605864
3	0.004115534665992703
4	0.01567068968974145
5	0.03682875893413984
6	0.04817286089809409
7	0.06822290157857136
8	0.09022518306214773
9	0.09755927689000653
10	0.12035300734781229
11	0.12151379917668202
12	0.13539053785817023
13	0.12283288080039763
14	0.1268956522014417
15	0.11750379104058656
16	0.11391588902408009
17	0.12568209710762335
18	0.1316971093117665
19	0.13428250929424912
20	0.121935905296271
21	0.13196092563650966
22	0.15375215406029152
23	0.14177489291695378
24	0.1353377745932216
25	0.13343829705507113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1895258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.81209521064583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59207863060368	42.034244346526506
2	12.69526084969492	13.409266494361452
3	3.4971100870991543	5.540691326459713
4	1.4136582666224762	2.9863301988873103
5	0.7194927664677965	1.8998960243034115
6	0.4394729692532519	1.3925692976824782
7	0.2807146891423443	1.0377591623008635
8	0.19903605050067527	0.840920867951406
9	0.14497926864867694	0.6890993045499354
>10	0.8222613142495598	8.052120825668126
>50	0.08722863769943304	3.253556900660865
>100	0.09145862892661158	10.483208774417992
>500	0.0123342119432642	4.468573647902243
>1k	0.00491362914812964	3.9117628283278973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3482	0.1837216885511102	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2313	0.12204143182616826	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2241	0.11824247674986729	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2154	0.11365207269933698	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2154	0.11365207269933698	No Hit
GAACTACGACGGTATCTGATCGTCT	2014	0.10626521560652956	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1967	0.10378534215394421	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1966	0.10373257888899559	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1953	0.10304665644466347	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1935	0.10209691767558823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.276326494862441E-5
6	0.0	0.0	0.0	0.0	1.0552652989724882E-4
7	0.0	0.0	0.0	1.5828979484587322E-4	1.0552652989724882E-4
8	0.0	0.0	0.0	1.5828979484587322E-4	1.0552652989724882E-4
9	0.0	0.0	0.0	1.5828979484587322E-4	1.0552652989724882E-4
10	0.0	0.0	0.0	2.1105305979449764E-4	1.5828979484587322E-4
11	0.0	0.0	0.0	2.1105305979449764E-4	1.5828979484587322E-4
12	0.0	0.0	0.0	2.6381632474312204E-4	4.748693845376197E-4
13	0.0	0.0	0.0	3.1657958969174643E-4	5.276326494862441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	365	0.0	14.839311	13
GCGTTAT	160	0.0	14.239624	1
CGGTCCA	430	0.0	13.920966	10
CGAACGA	105	1.36788E-9	13.574091	16
CGCCAGT	400	0.0	13.540872	18
GAAAGCG	100	1.0051735E-8	13.302258	9
ATCGCCA	425	0.0	12.967249	16
AACGGAC	105	1.9881554E-8	12.669486	15
GCATCGC	435	0.0	12.669152	14
GTGCGCT	55	0.0030661866	12.092321	11
AAAGCGT	95	1.03803E-6	12.002036	10
CCGGTCC	515	0.0	11.992327	9
GGTCCAA	510	0.0	11.922961	11
CGCAGTT	80	2.8598364E-5	11.877957	17
CAAGACG	505	0.0	11.848799	4
GTTATTC	185	0.0	11.804704	3
CGTTATT	185	0.0	11.802211	2
AGGCCCG	355	0.0	11.776646	10
CGTTCAA	105	2.7139686E-7	11.764212	14
CGCAAGA	485	0.0	11.7440195	2
>>END_MODULE