FastQCFastQC Report
Thu 2 Feb 2017
SRR4062610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895258
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22450.1184535298096618No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22400.11818971348491869No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20480.10805916661478279No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19670.10378534215394421No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19570.10325770950445796No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19310.10188586461579374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG500.002581126521.9997755
TTAACGG2750.019.99979635
GTCCTAC9000.019.3165191
TGTAGGA10450.019.1597212
TAGGACG7250.017.9037384
CTGTAGG10900.017.5645521
GTAGGAC11950.017.4898223
TCCTACA10000.017.3816592
ATTTCGT4100.017.17055542
TAACGGC3100.017.03208436
GATTTCG4200.016.76173441
CGCAATA6400.016.49983236
ATACCGT5100.016.391996
TTAGGAC5250.016.343123
GTATCAA39400.016.2532831
CTAGCGG6500.016.24598929
CTTACAC4350.016.184173
TACCGTC5450.016.1466267
TAGCGGC6700.015.76103430
GTCCTAA5350.015.6305451
Produced by FastQC (version 0.11.5)