FastQCFastQC Report
Thu 2 Feb 2017
SRR4062609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278319
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46291.6631994222456963No Hit
GTACATGGAAGCAGTGGTATCAACG40201.4443857587875781No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39211.4088150647278843No Hit
TCCATGTACTCTGCGTTGATACCAC33411.2004210995296762No Hit
TATCAACGCAGAGTACTTTTTTTTT29811.0710731211307887No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28721.0319094276711256No Hit
GTACATGGGGTGGTATCAACGCAAA23150.8317793610928467No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20950.7527333742935265No Hit
GAGTACATGGAAGCAGTGGTATCAA17650.6341643940945463No Hit
GGTATCAACGCAGAGTACTTTTTTT16640.5978751001548582No Hit
CATGTACTCTGCGTTGATACCACTG16110.578832203335022No Hit
GTACATGGGTGGTATCAACGCAAAA13790.4954746172557389No Hit
GTACATGGGAAGCAGTGGTATCAAC11750.42217742949636927No Hit
CCCATGTACTCTGCGTTGATACCAC11620.41750653027640944No Hit
GTATCAACGCAGAGTACATGGGGTG11300.40600893219650835No Hit
GCTTCCATGTACTCTGCGTTGATAC10810.3884032351366597No Hit
GTACATGGGAGTGGTATCAACGCAA9900.3557069405969409No Hit
GCGTTGATACCACTGCTTCCATGTA9610.34528724233703056No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9450.33953844329708No Hit
CATGGAAGCAGTGGTATCAACGCAG8760.3147467474372932No Hit
GTATCAACGCAGAGTACATGGGAAG8560.30756074863735494No Hit
GTATCAACGCAGAGTACATGGAAGC7690.2763016538576238No Hit
TATCAACGCAGAGTACATGGGGTGG7450.26767845529769796No Hit
ACTCTGCGTTGATACCACTGCTTCC7180.2579773569177814No Hit
CAGTGGTATCAACGCAGAGTACATG5980.2148613641181522No Hit
GGTATCAACGCAGAGTACATGGGGT5830.20947186501819853No Hit
ATCAACGCAGAGTACTTTTTTTTTT5570.2001300665782789No Hit
ACGCAGAGTACATGGAAGCAGTGGT5510.1979742669382974No Hit
ACGCAGAGTACATGGGGTGGTATCA5490.1972556670583036No Hit
TATCAACGCAGAGTACATGGGAAGC5260.18899176843837467No Hit
GTATCAACGCAGAGTACATGGGAGT5210.18719526873839012No Hit
GCGTTGATACCACTGCTTCCCATGT5100.1832429693984241No Hit
GGTATCAACGCAGAGTACATGGGAA4920.17677557047847972No Hit
TATCAACGCAGAGTACATGGAAGCA4760.17102677143852918No Hit
GAGTACATGGGGTGGTATCAACGCA4540.16312217275859714No Hit
GTATCAACGCAGAGTACATGGGTGG4280.15378037431867747No Hit
GAGTACATGGGAAGCAGTGGTATCA4170.14982807497871148No Hit
GGTGGTATCAACGCAAAAAAAAAAA4030.14479787581875475No Hit
GTATCAACGCAGAGTACATGGGCAG3920.14084557647878873No Hit
GGGTGGTATCAACGCAAAAAAAAAA3640.13078517815887525No Hit
ATACCACTGCTTCCATGTACTCTGC3560.12791077863889996No Hit
GGTATCAACGCAGAGTACATGGAAG3500.12575497899891852No Hit
TATCAACGCAGAGTACATGGGAGTG3280.1178503803189865No Hit
GTATTAGAGGCACTGCCTGCCCAGT3260.11713178043899267No Hit
AAGCAGTGGTATCAACGCAGAGTAC3250.11677248049899575No Hit
GTACTCTGCGTTGATACCACTGCTT3220.11569458067900502No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3150.11317948109902665No Hit
ACGCAGAGTACATGGGAAGCAGTGG3090.1110236814590452No Hit
GGTATCAACGCAGAGTACATGGGAG2950.10599348229908846No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2910.10455628253910082No Hit
CCATAGGGTCTTCTCGTCTTATTAT2910.10455628253910082No Hit
GTGGTATCAACGCAGAGTACATGGA2910.10455628253910082No Hit
AAAAAGTACTCTGCGTTGATACCAC2880.10347838271911008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCT307.6950755E-419.00215711
ACCTTGT250.00601595619.0021577
TTAGAAA250.00603473318.9919133
TATGCTC250.00603473318.9919133
GATATAC402.759856E-416.6179241
ACATGAG402.759856E-416.6179243
CTCTAGC508.704586E-515.193532
GCATTAC456.7318603E-414.7794567
TCTAGCA456.75946E-414.7714873
TCAGAAC456.75946E-414.7714873
GCCCAGT400.005254450314.25418219
AACTTAC400.005260726414.2516197
AGAACAG400.005260726414.2516195
AGAGGCA400.005260726414.2516196
TAAAGGA400.005267008714.2490564
TATACAC400.005279592214.2439353
TACATGA602.5636136E-514.2439342
AGTATTA500.001494901413.29911815
TCCTACA655.4428714E-513.1482472
TAAAGTG604.0693796E-412.6681055
Produced by FastQC (version 0.11.5)