##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062609_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 278319 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.04970196069977 32.0 32.0 32.0 21.0 32.0 2 30.143349897060567 32.0 32.0 32.0 21.0 32.0 3 30.306666091786763 32.0 32.0 32.0 21.0 32.0 4 30.068917321490808 32.0 32.0 32.0 21.0 32.0 5 30.200819922463072 32.0 32.0 32.0 21.0 32.0 6 33.5228640516817 36.0 36.0 36.0 21.0 36.0 7 33.09672354384717 36.0 36.0 36.0 14.0 36.0 8 32.97278302954523 36.0 36.0 36.0 14.0 36.0 9 32.961217164476736 36.0 36.0 36.0 14.0 36.0 10 32.94320545848469 36.0 36.0 36.0 14.0 36.0 11 33.46211721082643 36.0 36.0 36.0 21.0 36.0 12 33.229689672641825 36.0 36.0 36.0 21.0 36.0 13 33.32672580743679 36.0 36.0 36.0 21.0 36.0 14 33.309849489255136 36.0 36.0 36.0 21.0 36.0 15 33.42331641030616 36.0 36.0 36.0 21.0 36.0 16 33.33987259224128 36.0 36.0 36.0 21.0 36.0 17 33.2318167282866 36.0 36.0 36.0 21.0 36.0 18 33.39645873979139 36.0 36.0 36.0 21.0 36.0 19 33.2787089634556 36.0 36.0 36.0 21.0 36.0 20 33.02005612265063 36.0 36.0 36.0 14.0 36.0 21 32.88678458890698 36.0 36.0 36.0 14.0 36.0 22 32.98157869207636 36.0 36.0 36.0 14.0 36.0 23 33.09007649495722 36.0 36.0 36.0 14.0 36.0 24 33.13803944394741 36.0 36.0 36.0 21.0 36.0 25 32.67244061670242 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 13.0 5 22.0 6 45.0 7 22.0 8 24.0 9 45.0 10 38.0 11 15.0 12 12.0 13 12.0 14 275.0 15 275.0 16 329.0 17 389.0 18 649.0 19 976.0 20 1488.0 21 2046.0 22 2781.0 23 3549.0 24 4073.0 25 4887.0 26 5774.0 27 6594.0 28 7947.0 29 9323.0 30 11425.0 31 14716.0 32 19878.0 33 26787.0 34 55557.0 35 98350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.004671218109955 18.303629177146963 12.392382321236076 20.29931728350701 2 13.996931490311198 22.467671058635844 40.15867141435301 23.376726036699953 3 20.109664252040936 31.004584914338277 27.76208750143728 21.12366333218351 4 11.149082049568246 17.110639169478553 35.97956037558888 35.76071840536432 5 11.538890066958269 41.47998260450773 31.823327930188007 15.157799398345992 6 28.171515044769468 35.96654241686826 20.626664701674677 15.2352778366876 7 26.320084847831165 34.810260835175896 21.285660357008034 17.583993959984902 8 30.07224357844847 29.920852107434005 20.964302538414238 19.04260177570329 9 29.557583865584903 15.018484953104322 20.608205305253467 34.815725876057314 10 17.543443150712033 27.51009866658034 31.01936282179945 23.92709536090818 11 33.09256767936921 24.871763512492716 21.95271976460601 20.08294904353206 12 24.621644081027704 27.184427600646092 29.248972044650856 18.944956273675352 13 30.184566353600502 22.015230051474266 27.65617637219742 20.14402722272781 14 24.651699862946188 21.283269722618915 28.043511886989958 26.021518527444936 15 25.360223291681955 25.962333916021034 29.745847450921147 18.93159534137586 16 21.877765068951376 28.54414398860522 27.912179611685406 21.665911330757996 17 19.470723232206964 27.38649362234804 29.303746016215708 23.839037129229286 18 20.901729279760556 26.357216604251434 32.79767750569292 19.943376610295097 19 21.870357113307122 28.956863647973407 30.115584270754265 19.057194967965206 20 23.45033741492683 23.95502093555303 32.69219701002892 19.902444639491215 21 24.82184034160608 24.978685593619705 27.98824380083531 22.2112302639389 22 22.47161902020402 27.209758379360593 28.527787273087096 21.79083532734829 23 21.114153214055715 27.705962561746496 29.45986882580023 21.72001539839756 24 20.185344408908907 30.51599279056298 29.092452755523095 20.20621004500502 25 22.503102797014122 27.729472074826873 28.93029948736397 20.837125640795033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 424.0 1 424.0 2 791.0 3 1158.0 4 1158.0 5 1158.0 6 3060.0 7 4962.0 8 4962.0 9 4962.0 10 4615.0 11 4268.0 12 4268.0 13 4268.0 14 3007.5 15 1747.0 16 1747.0 17 1747.0 18 2717.5 19 3688.0 20 3688.0 21 3688.0 22 5571.5 23 7455.0 24 7455.0 25 7455.0 26 10470.0 27 13485.0 28 13485.0 29 13485.0 30 15880.5 31 18276.0 32 18276.0 33 18276.0 34 18219.0 35 18162.0 36 18162.0 37 18162.0 38 18450.0 39 18738.0 40 18738.0 41 18738.0 42 23238.5 43 27739.0 44 27739.0 45 27739.0 46 41156.5 47 54574.0 48 54574.0 49 54574.0 50 46804.5 51 39035.0 52 39035.0 53 39035.0 54 30471.0 55 21907.0 56 21907.0 57 21907.0 58 18949.5 59 15992.0 60 15992.0 61 15992.0 62 13558.0 63 11124.0 64 11124.0 65 11124.0 66 9263.5 67 7403.0 68 7403.0 69 7403.0 70 5730.0 71 4057.0 72 4057.0 73 4057.0 74 3268.5 75 2480.0 76 2480.0 77 2480.0 78 1736.5 79 993.0 80 993.0 81 993.0 82 660.5 83 328.0 84 328.0 85 328.0 86 219.5 87 111.0 88 111.0 89 111.0 90 79.0 91 47.0 92 47.0 93 47.0 94 29.0 95 11.0 96 11.0 97 11.0 98 83.0 99 155.0 100 155.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00682669885994129 2 0.0028743995199752804 3 0.00538949909995365 4 0.01293479783988876 5 0.030899794839734263 6 0.04167879303964156 7 0.06251818955946234 8 0.08335758607928312 9 0.09162148469921205 10 0.11210158127903593 11 0.11389808097902046 12 0.12216197959894941 13 0.11353878103902357 14 0.11713178043899267 15 0.10671208217908229 16 0.10671208217908229 17 0.11389808097902046 18 0.12288057947894324 19 0.12216197959894941 20 0.11605388061900193 21 0.1214433797189556 22 0.14515717575875164 23 0.1322223779188629 24 0.12575497899891852 25 0.12359917935893705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 278319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.00437813236697 #Duplication Level Percentage of deduplicated Percentage of total 1 72.80399999996489 26.212627455475808 2 13.558999999993462 9.763667261930566 3 5.025999999997576 5.428740134795674 4 2.3739999999988552 3.4189757474479188 5 1.3380000000473296 2.408692897140554 6 0.8579999999998364 1.8535053862538984 7 0.5799999999997216 1.4617777521733974 8 0.4669999999997748 1.3451235670245814 9 0.3709999999998211 1.2021861858391534 >10 2.2439999999989175 15.45703957599901 >50 0.223999999999892 5.53963361944331 >100 0.12299999999994068 8.483351575544214 >500 0.015999999999992284 4.052292758795948 >1k 0.015999999999992284 13.372386082135968 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4629 1.6631994222456963 No Hit GTACATGGAAGCAGTGGTATCAACG 4020 1.4443857587875781 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3921 1.4088150647278843 No Hit TCCATGTACTCTGCGTTGATACCAC 3341 1.2004210995296762 No Hit TATCAACGCAGAGTACTTTTTTTTT 2981 1.0710731211307887 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2872 1.0319094276711256 No Hit GTACATGGGGTGGTATCAACGCAAA 2315 0.8317793610928467 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2095 0.7527333742935265 No Hit GAGTACATGGAAGCAGTGGTATCAA 1765 0.6341643940945463 No Hit GGTATCAACGCAGAGTACTTTTTTT 1664 0.5978751001548582 No Hit CATGTACTCTGCGTTGATACCACTG 1611 0.578832203335022 No Hit GTACATGGGTGGTATCAACGCAAAA 1379 0.4954746172557389 No Hit GTACATGGGAAGCAGTGGTATCAAC 1175 0.42217742949636927 No Hit CCCATGTACTCTGCGTTGATACCAC 1162 0.41750653027640944 No Hit GTATCAACGCAGAGTACATGGGGTG 1130 0.40600893219650835 No Hit GCTTCCATGTACTCTGCGTTGATAC 1081 0.3884032351366597 No Hit GTACATGGGAGTGGTATCAACGCAA 990 0.3557069405969409 No Hit GCGTTGATACCACTGCTTCCATGTA 961 0.34528724233703056 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 945 0.33953844329708 No Hit CATGGAAGCAGTGGTATCAACGCAG 876 0.3147467474372932 No Hit GTATCAACGCAGAGTACATGGGAAG 856 0.30756074863735494 No Hit GTATCAACGCAGAGTACATGGAAGC 769 0.2763016538576238 No Hit TATCAACGCAGAGTACATGGGGTGG 745 0.26767845529769796 No Hit ACTCTGCGTTGATACCACTGCTTCC 718 0.2579773569177814 No Hit CAGTGGTATCAACGCAGAGTACATG 598 0.2148613641181522 No Hit GGTATCAACGCAGAGTACATGGGGT 583 0.20947186501819853 No Hit ATCAACGCAGAGTACTTTTTTTTTT 557 0.2001300665782789 No Hit ACGCAGAGTACATGGAAGCAGTGGT 551 0.1979742669382974 No Hit ACGCAGAGTACATGGGGTGGTATCA 549 0.1972556670583036 No Hit TATCAACGCAGAGTACATGGGAAGC 526 0.18899176843837467 No Hit GTATCAACGCAGAGTACATGGGAGT 521 0.18719526873839012 No Hit GCGTTGATACCACTGCTTCCCATGT 510 0.1832429693984241 No Hit GGTATCAACGCAGAGTACATGGGAA 492 0.17677557047847972 No Hit TATCAACGCAGAGTACATGGAAGCA 476 0.17102677143852918 No Hit GAGTACATGGGGTGGTATCAACGCA 454 0.16312217275859714 No Hit GTATCAACGCAGAGTACATGGGTGG 428 0.15378037431867747 No Hit GAGTACATGGGAAGCAGTGGTATCA 417 0.14982807497871148 No Hit GGTGGTATCAACGCAAAAAAAAAAA 403 0.14479787581875475 No Hit GTATCAACGCAGAGTACATGGGCAG 392 0.14084557647878873 No Hit GGGTGGTATCAACGCAAAAAAAAAA 364 0.13078517815887525 No Hit ATACCACTGCTTCCATGTACTCTGC 356 0.12791077863889996 No Hit GGTATCAACGCAGAGTACATGGAAG 350 0.12575497899891852 No Hit TATCAACGCAGAGTACATGGGAGTG 328 0.1178503803189865 No Hit GTATTAGAGGCACTGCCTGCCCAGT 326 0.11713178043899267 No Hit AAGCAGTGGTATCAACGCAGAGTAC 325 0.11677248049899575 No Hit GTACTCTGCGTTGATACCACTGCTT 322 0.11569458067900502 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 315 0.11317948109902665 No Hit ACGCAGAGTACATGGGAAGCAGTGG 309 0.1110236814590452 No Hit GGTATCAACGCAGAGTACATGGGAG 295 0.10599348229908846 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 291 0.10455628253910082 No Hit CCATAGGGTCTTCTCGTCTTATTAT 291 0.10455628253910082 No Hit GTGGTATCAACGCAGAGTACATGGA 291 0.10455628253910082 No Hit AAAAAGTACTCTGCGTTGATACCAC 288 0.10347838271911008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACCT 30 7.6950755E-4 19.002157 11 ACCTTGT 25 0.006015956 19.002157 7 TTAGAAA 25 0.006034733 18.991913 3 TATGCTC 25 0.006034733 18.991913 3 GATATAC 40 2.759856E-4 16.617924 1 ACATGAG 40 2.759856E-4 16.617924 3 CTCTAGC 50 8.704586E-5 15.19353 2 GCATTAC 45 6.7318603E-4 14.779456 7 TCTAGCA 45 6.75946E-4 14.771487 3 TCAGAAC 45 6.75946E-4 14.771487 3 GCCCAGT 40 0.0052544503 14.254182 19 AACTTAC 40 0.0052607264 14.251619 7 AGAACAG 40 0.0052607264 14.251619 5 AGAGGCA 40 0.0052607264 14.251619 6 TAAAGGA 40 0.0052670087 14.249056 4 TATACAC 40 0.0052795922 14.243935 3 TACATGA 60 2.5636136E-5 14.243934 2 AGTATTA 50 0.0014949014 13.299118 15 TCCTACA 65 5.4428714E-5 13.148247 2 TAAAGTG 60 4.0693796E-4 12.668105 5 >>END_MODULE