##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278319
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.57828247442683	32.0	32.0	32.0	32.0	32.0
2	31.270189961878277	32.0	32.0	32.0	32.0	32.0
3	31.451079516669722	32.0	32.0	32.0	32.0	32.0
4	31.616745533003495	32.0	32.0	32.0	32.0	32.0
5	31.537915126168173	32.0	32.0	32.0	32.0	32.0
6	35.089325558082635	36.0	36.0	36.0	36.0	36.0
7	35.06110973379467	36.0	36.0	36.0	36.0	36.0
8	35.05024809660857	36.0	36.0	36.0	36.0	36.0
9	35.03380653135431	36.0	36.0	36.0	36.0	36.0
10	35.00516673313716	36.0	36.0	36.0	36.0	36.0
11	35.10718995109928	36.0	36.0	36.0	36.0	36.0
12	35.03000873098854	36.0	36.0	36.0	36.0	36.0
13	35.02811162730536	36.0	36.0	36.0	36.0	36.0
14	34.980964289178964	36.0	36.0	36.0	36.0	36.0
15	35.026440882584374	36.0	36.0	36.0	36.0	36.0
16	35.00553321907595	36.0	36.0	36.0	36.0	36.0
17	34.9885598899105	36.0	36.0	36.0	36.0	36.0
18	34.98467945055853	36.0	36.0	36.0	36.0	36.0
19	34.90056014860645	36.0	36.0	36.0	32.0	36.0
20	34.779468882828695	36.0	36.0	36.0	32.0	36.0
21	34.705090202249934	36.0	36.0	36.0	32.0	36.0
22	34.750699736633145	36.0	36.0	36.0	32.0	36.0
23	34.743876630772604	36.0	36.0	36.0	32.0	36.0
24	34.730162152062924	36.0	36.0	36.0	32.0	36.0
25	34.711880971115875	36.0	36.0	36.0	32.0	36.0
26	34.688641450996876	36.0	36.0	36.0	32.0	36.0
27	34.674988053277	36.0	36.0	36.0	32.0	36.0
28	34.61418731743072	36.0	36.0	36.0	32.0	36.0
29	34.55148947790126	36.0	36.0	36.0	32.0	36.0
30	34.500167074472095	36.0	36.0	36.0	32.0	36.0
31	34.430937162033494	36.0	36.0	36.0	32.0	36.0
32	34.3250622487146	36.0	36.0	36.0	32.0	36.0
33	34.16035197022122	36.0	36.0	36.0	32.0	36.0
34	34.00977655136732	36.0	36.0	36.0	32.0	36.0
35	33.86741472914174	36.0	36.0	36.0	32.0	36.0
36	33.80055619630711	36.0	36.0	36.0	32.0	36.0
37	33.66464740100388	36.0	36.0	36.0	27.0	36.0
38	33.55976774851879	36.0	36.0	36.0	21.0	36.0
39	33.35401823087896	36.0	36.0	36.0	21.0	36.0
40	33.17566892666329	36.0	36.0	36.0	14.0	36.0
41	33.143252886076766	36.0	36.0	36.0	14.0	36.0
42	32.873918058055686	36.0	36.0	36.0	14.0	36.0
43	32.814676683948996	36.0	36.0	36.0	14.0	36.0
44	32.70796460176991	36.0	36.0	36.0	14.0	36.0
45	32.373132987686795	36.0	36.0	36.0	14.0	36.0
46	32.31731214900887	36.0	36.0	36.0	14.0	36.0
47	32.02966380304615	36.0	36.0	36.0	14.0	36.0
48	31.879774647077635	36.0	36.0	36.0	14.0	36.0
49	31.785393738839247	36.0	36.0	36.0	14.0	36.0
50	31.221537157003294	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	16.0
22	43.0
23	154.0
24	387.0
25	1060.0
26	2307.0
27	4443.0
28	7404.0
29	10213.0
30	12508.0
31	15689.0
32	21279.0
33	26196.0
34	42416.0
35	134199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.94664443518132	19.052119417573415	13.194075117505857	20.8071610297394
2	13.806385776806824	22.838170934151616	39.204329047882545	24.15111424115901
3	19.798287564180413	30.44190617173942	28.550178393702147	21.20962787037802
4	11.014698960545273	16.90110987751465	35.96304959417071	36.121141567769364
5	11.131830740984267	40.709042501589906	32.577725559519834	15.581401197906
6	28.42550041858744	36.36285243086122	19.546416496297386	15.665230654253962
7	25.264893880762724	34.26176437828535	22.103054408789912	18.370287332162015
8	29.676378544044784	29.470499678426552	21.370441831136215	19.482679946392448
9	28.812980788232206	14.055454352739122	21.413198523995845	35.71836633503282
10	17.33478490509092	27.122833870486744	31.621628419188053	23.920752805234283
11	32.90504780485702	23.98470819455373	22.542478235406136	20.567765765183115
12	24.006625490893544	26.930967702528392	30.340724133099066	18.72168267347899
13	30.398212123498574	21.440146019495614	28.47452024475512	19.687121612250692
14	24.345804634250626	20.416500490444417	29.477326377286495	25.760368498018458
15	25.295793675602457	25.253755582622816	30.544087899137324	18.906362842637407
16	21.696326876713414	27.192538058846143	29.169047028769146	21.942088035671297
17	19.399322360313164	25.71294090593887	31.150586197852103	23.73715053589586
18	21.114260973918416	23.639061652276705	35.33930489833608	19.907372475468797
19	21.73944286951304	26.863778613748973	32.76060922897826	18.636169287759728
20	22.772070897064157	22.163416798709395	35.56350806089416	19.50100424333229
21	24.064472781233047	23.370305297159017	31.011177821133302	21.554044100474634
22	21.74986256777295	25.027396620424764	31.756725196626896	21.466015615175394
23	20.551956567823254	25.1276413036839	32.557604762880004	21.76279736561284
24	19.887610978768965	27.881675343760215	32.061770845684265	20.168942831786545
25	22.32474247176801	24.876490645626063	31.921284569145474	20.877482313460455
26	19.180508696855046	25.156025998943658	33.82701145088909	21.836453853312207
27	21.52098850599492	26.701015740930373	33.12996956729508	18.648026185779624
28	20.192656627826345	24.664862980967882	33.55466209637143	21.587818294834346
29	19.226499089174652	26.545798166851707	34.16690919412616	20.060793549847478
30	21.280616846136986	25.671621412839222	33.68688447429029	19.360877266733496
31	19.02205742331641	26.455973181852478	32.69845033935879	21.823519055472318
32	18.83342495481803	27.426442319784133	32.70491773827874	21.035214987119097
33	19.75431070103011	26.780780327609687	32.549700164200075	20.91520880716013
34	19.19919229373489	27.488960509343595	33.75694796258969	19.554899234331828
35	21.199774359637683	27.279488644325394	31.74594619842698	19.77479079760994
36	20.407877291884493	28.053780015018738	31.481860742529257	20.056481950567516
37	21.661834082473707	28.226243986217252	30.913448237454144	19.198473693854893
38	20.68813124508208	28.605305422913997	30.156403263880655	20.55016006812327
39	19.702930809610557	28.474160944815125	31.364369662150267	20.45853858342406
40	20.83616282036081	28.80435758967228	30.124784869160926	20.234694720805983
41	19.15859140051524	28.67932121055336	31.489765341209186	20.67232204772222
42	21.389125428016055	29.635418350885136	29.8251287192035	19.150327501895305
43	20.199483326686284	28.30816437253655	28.931549768431186	22.560802532345978
44	19.471541648252543	28.76950549549258	31.226398485191453	20.53255437106342
45	20.931736604399987	28.64626561607364	29.256716214128392	21.16528156539798
46	20.14709792395749	28.44911216665828	29.806552217247894	21.597237692136336
47	21.445176218655572	28.43823095081543	28.91394407137134	21.202648759157658
48	20.1049874424671	32.10380893866391	26.164580930514987	21.62662268835401
49	19.613824424491323	29.330372701827756	29.852435514643272	21.20336735903765
50	18.809351858838237	32.168842227803346	26.163143730754996	22.858662182603414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	38.5
2	41.0
3	171.5
4	302.0
5	352.0
6	402.0
7	398.0
8	394.0
9	540.5
10	687.0
11	986.0
12	1285.0
13	2252.0
14	3219.0
15	4560.0
16	5901.0
17	6677.5
18	7454.0
19	7769.0
20	8084.0
21	8068.5
22	8053.0
23	7637.5
24	7222.0
25	5941.0
26	4660.0
27	4924.0
28	5188.0
29	5683.5
30	6179.0
31	6788.0
32	7397.0
33	8167.5
34	8938.0
35	9309.0
36	9680.0
37	10346.0
38	11012.0
39	10829.5
40	10647.0
41	11553.5
42	12460.0
43	13295.0
44	14130.0
45	16842.0
46	19554.0
47	21330.0
48	23106.0
49	21607.5
50	20109.0
51	17870.5
52	15632.0
53	14226.5
54	12821.0
55	12138.5
56	11456.0
57	10898.0
58	10340.0
59	9371.0
60	8402.0
61	7474.0
62	6546.0
63	5665.0
64	4784.0
65	4364.5
66	3945.0
67	3386.0
68	2827.0
69	2462.5
70	2098.0
71	1700.5
72	1303.0
73	1060.0
74	817.0
75	724.5
76	632.0
77	466.0
78	300.0
79	229.0
80	158.0
81	115.5
82	73.0
83	49.0
84	25.0
85	18.5
86	12.0
87	7.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01257549789989185
2	0.00467089921995983
3	7.185998799938201E-4
4	0.0
5	0.0
6	7.185998799938201E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.5929993999691005E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	278319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.25621294341432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09630769377036	35.9602332058439
2	12.503881063704156	11.817721323310591
3	4.286530522405645	6.076955974657385
4	2.105212152929233	3.9793741515955023
5	1.1618350400308621	2.745196202840936
6	0.6985704078516406	1.9807075169642954
7	0.5175794624639731	1.7121191705334802
8	0.3734580841057038	1.4118571798350938
9	0.27055521918500564	1.1506873545682885
>10	1.7490529287303838	15.583532174365002
>50	0.13794261701920804	4.641203697653983
>100	0.08764309921109903	7.884319961902772
>500	0.00838325296801817	2.7234253403941513
>1k	0.0030484556247338795	2.332666745534636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1843	0.6621897894143052	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1726	0.6201516964346666	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1523	0.5472138086152939	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1385	0.4976304168957204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.2985782501374322	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	785	0.28205045289757436	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	773	0.2777388536176114	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	747	0.2683970551776918	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	729	0.2619296562577474	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	695	0.24971345829785246	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	673	0.24180885961792045	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	616	0.22132876303809657	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	614	0.22061016315810278	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.20264516615825726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	535	0.19222546789834685	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	492	0.17677557047847972	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	463	0.16635587221856935	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	449	0.16132567305861262	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	426	0.15306177443868366	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	404	0.14515717575875164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	392	0.14084557647878873	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	364	0.13078517815887525	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	341	0.12252127953894631	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	336	0.12072477983896177	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	331	0.11892828013897722	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	330	0.11856898019898031	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	322	0.11569458067900502	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	315	0.11317948109902665	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	308	0.11066438151904828	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	300	0.107789981999073	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	291	0.10455628253910082	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	285	0.10240048289911936	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	279	0.1002446832591379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	3.5929993999691E-4	0.0	0.0	0.0	0.0
11	3.5929993999691E-4	0.0	0.0	0.0	0.0
12	3.5929993999691E-4	0.0	0.0	0.0	0.0
13	3.5929993999691E-4	0.0	0.0	0.0	0.0
14	3.5929993999691E-4	0.0	0.0	0.0	0.0
15	3.5929993999691E-4	0.0	0.0	0.0	0.0
16	3.5929993999691E-4	0.0	0.0	7.1859987999382E-4	0.0
17	3.5929993999691E-4	0.0	0.0	0.00107789981999073	0.0
18	3.5929993999691E-4	0.0	0.0	0.00143719975998764	0.0
19	3.5929993999691E-4	0.0	0.0	0.0017964996999845502	0.0
20	3.5929993999691E-4	0.0	0.0	0.00323369945997219	0.0
21	3.5929993999691E-4	0.0	0.0	0.0050301991599567405	0.0
22	3.5929993999691E-4	0.0	0.0	0.00826389861992893	0.0
23	3.5929993999691E-4	0.0	0.0	0.020480096579823873	0.0
24	3.5929993999691E-4	0.0	0.0	0.03449279423970336	0.0
25	3.5929993999691E-4	0.0	0.0	0.04347529273962611	0.0
26	3.5929993999691E-4	0.0	0.0	0.052098491299551956	0.0
27	3.5929993999691E-4	0.0	0.0	0.07437508757936037	0.0
28	3.5929993999691E-4	0.0	0.0	0.11210158127903593	0.0
29	3.5929993999691E-4	0.0	0.0	0.16779307197855697	0.0
30	3.5929993999691E-4	0.0	0.0	0.2727086544576547	0.0
31	3.5929993999691E-4	0.0	0.0	0.4218181295563724	0.0
32	3.5929993999691E-4	0.0	0.0	0.6176365968546883	0.0
33	3.5929993999691E-4	0.0	0.0	0.7556077738135017	0.0
34	3.5929993999691E-4	0.0	0.0	0.9338205440519691	0.0
35	3.5929993999691E-4	0.0	0.0	1.1332320107502543	0.0
36	3.5929993999691E-4	0.0	0.0	1.4242649621477512	0.0
37	3.5929993999691E-4	0.0	0.0	1.8105123976444295	0.0
38	3.5929993999691E-4	0.0	0.0	2.244546725160697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCG	20	7.846505E-4	43.999638	44
TAGGCCC	20	7.846505E-4	43.999638	4
GTAGTCC	20	7.846505E-4	43.999638	4
AGACGTT	30	1.2986243E-4	36.66637	42
CTAGGTA	30	1.2986243E-4	36.66637	4
CCGGTAT	35	3.211703E-4	31.428316	28
TCCTACA	80	1.2914825E-10	30.249752	2
ATACATG	125	0.0	29.93051	1
CGACGGA	30	0.005737744	29.333096	31
TGAACAC	30	0.005737744	29.333096	5
GCACGAA	30	0.005737744	29.333096	43
GATCCTC	40	7.018782E-4	27.499773	6
CCTACAG	80	4.285539E-9	27.499773	3
TGGCCGG	40	7.018782E-4	27.499773	25
AACATAA	40	7.018782E-4	27.499773	25
ATAACAT	40	7.018782E-4	27.499773	3
TCTACTT	50	8.319262E-5	26.399784	2
GATATAC	80	1.297849E-7	24.758694	1
AGATCCT	45	0.0013955802	24.444244	5
GCCGGTA	45	0.0013955802	24.444244	27
>>END_MODULE