FastQCFastQC Report
Thu 2 Feb 2017
SRR4062608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647035
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG70111.0835580764564514No Hit
TCCATGTACTCTGCGTTGATACCAC61370.9484803758683843No Hit
GAGTACATGGAAGCAGTGGTATCAA31420.4855996970797561No Hit
CATGTACTCTGCGTTGATACCACTG28230.43629788187655993No Hit
GCTTCCATGTACTCTGCGTTGATAC20220.3125024148616381No Hit
CCCATGTACTCTGCGTTGATACCAC17310.26752803171389494No Hit
GCGTTGATACCACTGCTTCCATGTA16890.26103688363071553No Hit
CATGGAAGCAGTGGTATCAACGCAG16830.2601095767616899No Hit
GTACATGGGAAGCAGTGGTATCAAC14700.2271901829112799No Hit
GTATCAACGCAGAGTACTTTTTTTT13990.21621705162780994No Hit
GTATCAACGCAGAGTACATGGAAGC13260.20493481805466474No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12540.19380713562635715No Hit
CAGTGGTATCAACGCAGAGTACATG10410.1608877417759472No Hit
ACGCAGAGTACATGGAAGCAGTGGT10410.1608877417759472No Hit
ACTCTGCGTTGATACCACTGCTTCC10370.16026953719659678No Hit
TATCAACGCAGAGTACTTTTTTTTT9440.1458962807266995No Hit
TATCAACGCAGAGTACATGGAAGCA9080.1403324395125457No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8590.13275943341550303No Hit
GCGCAAGACGGACCAGAGCGAAAGC8170.1262682853323236No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8070.12472277388394755No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7470.11544970519369122No Hit
GAATAACGCCGCCGCATCGCCAGTC7150.11050406855888785No Hit
ATACCACTGCTTCCATGTACTCTGC6690.103394715896358No Hit
GAATAGGACCGCGGTTCTATTTTGT6520.10076734643411872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC350.002176154616.2769643
AAGACGG1450.015.06436355
CAAGACG1400.014.9228584
TAGACAG456.770432E-414.7732655
ACCGTCG1058.731149E-1114.477378
CCGCATC1006.548362E-1014.25336612
GCCGTTA400.005271837614.2511615
TCGCGTA400.005271837614.251169
CGCATCG954.8748916E-914.00114113
TTCGTTC551.9508803E-413.82358718
TTTCGTT551.952162E-413.82251617
GAACCGC551.9572968E-413.8182376
GCTCTAC551.9650211E-413.8118241
AGGCCCG1400.013.573585510
GCAAGAC1850.013.3441783
CTTTCGT500.001495229713.30314316
CCGTTAA500.001495229713.30314316
GCATCGC1001.0018994E-813.30108314
TAAAGAG500.001501346813.29593855
GTCCTAA1500.013.2938821
Produced by FastQC (version 0.11.5)