##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062608_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647035
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127189410155555	32.0	32.0	32.0	32.0	32.0
2	30.829749549869792	32.0	32.0	32.0	32.0	32.0
3	30.821428516231734	32.0	32.0	32.0	32.0	32.0
4	30.86760067075197	32.0	32.0	32.0	32.0	32.0
5	30.696237452378927	32.0	32.0	32.0	32.0	32.0
6	34.32708276986562	36.0	36.0	36.0	32.0	36.0
7	34.18973007642554	36.0	36.0	36.0	32.0	36.0
8	34.19953016451969	36.0	36.0	36.0	32.0	36.0
9	34.33039325538804	36.0	36.0	36.0	32.0	36.0
10	34.04539630777315	36.0	36.0	36.0	32.0	36.0
11	34.33125719628768	36.0	36.0	36.0	32.0	36.0
12	34.124571313762004	36.0	36.0	36.0	32.0	36.0
13	34.24050940057339	36.0	36.0	36.0	32.0	36.0
14	34.139969244322174	36.0	36.0	36.0	32.0	36.0
15	34.11134328127535	36.0	36.0	36.0	32.0	36.0
16	34.08752540434443	36.0	36.0	36.0	32.0	36.0
17	34.00776928605099	36.0	36.0	36.0	32.0	36.0
18	34.03654207268541	36.0	36.0	36.0	32.0	36.0
19	34.015373202377	36.0	36.0	36.0	32.0	36.0
20	34.020274019179794	36.0	36.0	36.0	32.0	36.0
21	33.96283354068945	36.0	36.0	36.0	32.0	36.0
22	33.913136074555474	36.0	36.0	36.0	32.0	36.0
23	33.844778103193796	36.0	36.0	36.0	32.0	36.0
24	33.82963827304551	36.0	36.0	36.0	32.0	36.0
25	33.29967776086301	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	34.0
5	65.0
6	116.0
7	33.0
8	96.0
9	90.0
10	101.0
11	22.0
12	52.0
13	50.0
14	111.0
15	132.0
16	236.0
17	306.0
18	411.0
19	570.0
20	892.0
21	1396.0
22	1902.0
23	2838.0
24	4053.0
25	5383.0
26	7591.0
27	9897.0
28	13432.0
29	18059.0
30	23478.0
31	32476.0
32	46696.0
33	67048.0
34	146735.0
35	262732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.263835285716716	16.90513101499721	11.291107858426832	25.539925840859247
2	15.389180742890792	20.530344830783925	37.54499523193506	26.53547919439022
3	19.564088455855533	24.751318368129187	28.218178333632142	27.46641484238314
4	12.275554621225236	15.56231914525264	35.806457596517696	36.35566863700443
5	13.566051475669886	37.3494683754103	34.339377016687	14.745103132232822
6	34.019260386321044	34.85471211474589	17.50072674868103	13.625300750252043
7	29.070477775320487	30.809107935187235	21.552549408367756	18.567864881124528
8	27.900245657675722	33.3902615604522	20.563465691461385	18.146027090410687
9	26.8236007586798	14.939834711766654	18.619988675497304	39.616575854056244
10	15.275216013468345	27.51974449753658	32.65331385704736	24.55172563194771
11	36.95615843128516	21.269629051925303	21.802251808936674	19.97196070785286
12	23.28132399414392	24.16855730570782	30.001423782743135	22.548694917405125
13	30.351713071210717	19.72712797317393	25.235014398742255	24.686144556873096
14	23.441990018956247	19.916128283492593	25.13288715230763	31.508994545243528
15	25.269306348543225	27.23374955515326	23.973912639836605	23.52303145646691
16	25.658069826887253	25.638728484690944	25.382030191061517	23.321171497360293
17	23.39341644279931	25.97645369350186	27.059992324547828	23.570137539150995
18	24.897939602529302	24.233115542583715	28.53234208107263	22.336602773814352
19	24.619608280097065	27.235613826573562	25.17781533204576	22.966962561283612
20	24.224445424510417	24.452078642557044	27.713377126807643	23.610098806124896
21	25.25820738920373	25.714422782294882	23.961521914742814	25.065847913758578
22	23.940291669375334	26.27841392781632	25.198351891578984	24.582942511229362
23	22.863059365394793	25.545784620527627	25.38482729588599	26.206328718191585
24	23.294792409847204	27.107047402085882	25.386711815932138	24.21144837213477
25	24.768143460895054	25.158581670914597	24.99794954170323	25.075325326487125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	88.0
2	257.0
3	426.0
4	426.0
5	426.0
6	1189.0
7	1952.0
8	1952.0
9	1952.0
10	1959.0
11	1966.0
12	1966.0
13	1966.0
14	2010.5
15	2055.0
16	2055.0
17	2055.0
18	3276.0
19	4497.0
20	4497.0
21	4497.0
22	7420.5
23	10344.0
24	10344.0
25	10344.0
26	15456.5
27	20569.0
28	20569.0
29	20569.0
30	26448.5
31	32328.0
32	32328.0
33	32328.0
34	37684.0
35	43040.0
36	43040.0
37	43040.0
38	47786.5
39	52533.0
40	52533.0
41	52533.0
42	60999.0
43	69465.0
44	69465.0
45	69465.0
46	89141.0
47	108817.0
48	108817.0
49	108817.0
50	97074.0
51	85331.0
52	85331.0
53	85331.0
54	74708.0
55	64085.0
56	64085.0
57	64085.0
58	58017.5
59	51950.0
60	51950.0
61	51950.0
62	46361.5
63	40773.0
64	40773.0
65	40773.0
66	33635.0
67	26497.0
68	26497.0
69	26497.0
70	20031.0
71	13565.0
72	13565.0
73	13565.0
74	10641.0
75	7717.0
76	7717.0
77	7717.0
78	6460.0
79	5203.0
80	5203.0
81	5203.0
82	3654.0
83	2105.0
84	2105.0
85	2105.0
86	1558.5
87	1012.0
88	1012.0
89	1012.0
90	671.5
91	331.0
92	331.0
93	331.0
94	200.5
95	70.0
96	70.0
97	70.0
98	193.0
99	316.0
100	316.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0074184549522050575
2	0.003400125186427319
3	0.003554676331264924
4	0.016536972497623776
5	0.03771047934037571
6	0.04930181520319611
7	0.06707519685952074
8	0.09381254491642646
9	0.1003036929996059
10	0.1203953418284946
11	0.1234863647252467
12	0.1343049448638791
13	0.12333181358040911
14	0.12518642731846036
15	0.11514060290401601
16	0.11606790977304164
17	0.1265773876219988
18	0.13121392196712697
19	0.13275943341550303
20	0.12673193876683642
21	0.13121392196712697
22	0.14852365018893876
23	0.13925058149868247
24	0.13631410974676794
25	0.1295138593739133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	647035.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.9904387498803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.74382510242098	45.5011924622549
2	11.210928705345166	12.329877766011164
3	2.778937990479608	4.58445058065553
4	1.028249702298332	2.2617560909527628
5	0.49533600521275084	1.3619372127631082
6	0.3049109506500472	1.0060312173533517
7	0.20389364321647813	0.7848540629149982
8	0.15749629396826773	0.6928632245436134
9	0.11149636276599423	0.5518110516766056
>10	0.7436205173261419	8.04242623351059
>50	0.1100892561612446	4.255504844167692
>100	0.10023470221560374	10.820453802447634
>500	0.006757395654983752	2.417826268194087
>1k	0.003660255979782866	3.353309510537112
>5k	5.631163045819793E-4	2.0357056720168965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7011	1.0835580764564514	No Hit
TCCATGTACTCTGCGTTGATACCAC	6137	0.9484803758683843	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3142	0.4855996970797561	No Hit
CATGTACTCTGCGTTGATACCACTG	2823	0.43629788187655993	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2022	0.3125024148616381	No Hit
CCCATGTACTCTGCGTTGATACCAC	1731	0.26752803171389494	No Hit
GCGTTGATACCACTGCTTCCATGTA	1689	0.26103688363071553	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1683	0.2601095767616899	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1470	0.2271901829112799	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1399	0.21621705162780994	No Hit
GTATCAACGCAGAGTACATGGAAGC	1326	0.20493481805466474	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1254	0.19380713562635715	No Hit
CAGTGGTATCAACGCAGAGTACATG	1041	0.1608877417759472	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1041	0.1608877417759472	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1037	0.16026953719659678	No Hit
TATCAACGCAGAGTACTTTTTTTTT	944	0.1458962807266995	No Hit
TATCAACGCAGAGTACATGGAAGCA	908	0.1403324395125457	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	859	0.13275943341550303	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	817	0.1262682853323236	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	807	0.12472277388394755	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	747	0.11544970519369122	No Hit
GAATAACGCCGCCGCATCGCCAGTC	715	0.11050406855888785	No Hit
ATACCACTGCTTCCATGTACTCTGC	669	0.103394715896358	No Hit
GAATAGGACCGCGGTTCTATTTTGT	652	0.10076734643411872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5455114483760537E-4
2	0.0	0.0	0.0	0.0	1.5455114483760537E-4
3	0.0	0.0	0.0	0.0	1.5455114483760537E-4
4	0.0	0.0	0.0	0.0	1.5455114483760537E-4
5	0.0	0.0	0.0	0.0	1.5455114483760537E-4
6	0.0	0.0	0.0	0.0	1.5455114483760537E-4
7	0.0	0.0	0.0	0.0	1.5455114483760537E-4
8	0.0	0.0	0.0	0.0	1.5455114483760537E-4
9	0.0	0.0	0.0	0.0	1.5455114483760537E-4
10	0.0	0.0	0.0	0.0	1.5455114483760537E-4
11	0.0	0.0	0.0	0.0	1.5455114483760537E-4
12	0.0	0.0	0.0	0.0	3.0910228967521075E-4
13	0.0	0.0	0.0	0.0	3.0910228967521075E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	35	0.0021761546	16.276964	3
AAGACGG	145	0.0	15.0643635	5
CAAGACG	140	0.0	14.922858	4
TAGACAG	45	6.770432E-4	14.773265	5
ACCGTCG	105	8.731149E-11	14.47737	8
CCGCATC	100	6.548362E-10	14.253366	12
GCCGTTA	40	0.0052718376	14.25116	15
TCGCGTA	40	0.0052718376	14.25116	9
CGCATCG	95	4.8748916E-9	14.001141	13
TTCGTTC	55	1.9508803E-4	13.823587	18
TTTCGTT	55	1.952162E-4	13.822516	17
GAACCGC	55	1.9572968E-4	13.818237	6
GCTCTAC	55	1.9650211E-4	13.811824	1
AGGCCCG	140	0.0	13.5735855	10
GCAAGAC	185	0.0	13.344178	3
CTTTCGT	50	0.0014952297	13.303143	16
CCGTTAA	50	0.0014952297	13.303143	16
GCATCGC	100	1.0018994E-8	13.301083	14
TAAAGAG	50	0.0015013468	13.2959385	5
GTCCTAA	150	0.0	13.293882	1
>>END_MODULE