FastQCFastQC Report
Thu 2 Feb 2017
SRR4062608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647035
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42310.6539058938079083No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33270.5141916588747131No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC16020.24759093402984383No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15400.23800876304991228No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10700.16536972497623775No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA9910.15316018453406693No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG9160.14156884867124653No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC9140.14125974638157132No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8440.13044116624293894No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7440.1149860517591784No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7160.11065861970372545No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7140.11034951741405025No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7040.1088040059656742No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG6990.10803125024148616No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6930.10710394337246053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG300.00574316429.333239
TAACGGC952.6804628E-823.15781236
TTGACGG1053.0631782E-923.04753733
CGCGGGA1254.0017767E-1122.8799244
TGTAGGA4200.022.525472
TGGTATA2150.022.51154744
ATTGACG1205.966285E-1021.99992232
CGCAATA2550.021.5685536
CTAAGAC755.4677446E-520.5332623
GTAGGAC4300.020.4650443
ATACGAA2700.020.37029840
CCCTATA654.9263035E-420.309192
CTATACT654.928872E-420.3076214
CTAATCC654.928872E-420.3076213
GTCTTAC550.004479486520.0014741
CCGTCCA550.004481490719.999939
TCTATAC550.004481490719.999933
ATCGTTT1553.274181E-1119.87089729
AAGTACT1900.019.6841414
CTGTAGG4300.019.4432951
Produced by FastQC (version 0.11.5)