##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647035
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22661679816393	32.0	32.0	32.0	32.0	32.0
2	31.367239793828773	32.0	32.0	32.0	32.0	32.0
3	31.472960504454935	32.0	32.0	32.0	32.0	32.0
4	31.588042377923916	32.0	32.0	32.0	32.0	32.0
5	31.5132519879141	32.0	32.0	32.0	32.0	32.0
6	35.10130363890671	36.0	36.0	36.0	36.0	36.0
7	35.1182007155718	36.0	36.0	36.0	36.0	36.0
8	35.06203682953782	36.0	36.0	36.0	36.0	36.0
9	35.18888622717473	36.0	36.0	36.0	36.0	36.0
10	35.066167981639325	36.0	36.0	36.0	36.0	36.0
11	35.198452943040174	36.0	36.0	36.0	36.0	36.0
12	35.102572503805824	36.0	36.0	36.0	36.0	36.0
13	35.137647886126715	36.0	36.0	36.0	36.0	36.0
14	35.11107281677189	36.0	36.0	36.0	36.0	36.0
15	35.06345406353598	36.0	36.0	36.0	36.0	36.0
16	35.074685295231326	36.0	36.0	36.0	36.0	36.0
17	35.047261740091336	36.0	36.0	36.0	36.0	36.0
18	35.06850788597217	36.0	36.0	36.0	36.0	36.0
19	35.046335978733765	36.0	36.0	36.0	36.0	36.0
20	35.03055476133439	36.0	36.0	36.0	36.0	36.0
21	34.997919741590486	36.0	36.0	36.0	36.0	36.0
22	34.97261971918057	36.0	36.0	36.0	36.0	36.0
23	34.90227885663063	36.0	36.0	36.0	32.0	36.0
24	34.88696747471157	36.0	36.0	36.0	32.0	36.0
25	34.859275000579565	36.0	36.0	36.0	32.0	36.0
26	34.79820875223133	36.0	36.0	36.0	32.0	36.0
27	34.77237398286028	36.0	36.0	36.0	32.0	36.0
28	34.73979151050561	36.0	36.0	36.0	32.0	36.0
29	34.71435548308824	36.0	36.0	36.0	32.0	36.0
30	34.68642190917029	36.0	36.0	36.0	32.0	36.0
31	34.66431182238983	36.0	36.0	36.0	32.0	36.0
32	34.620308020431665	36.0	36.0	36.0	32.0	36.0
33	34.59655814600447	36.0	36.0	36.0	32.0	36.0
34	34.56427395735934	36.0	36.0	36.0	32.0	36.0
35	34.49309079107004	36.0	36.0	36.0	32.0	36.0
36	34.456591992705185	36.0	36.0	36.0	32.0	36.0
37	34.39996290772524	36.0	36.0	36.0	32.0	36.0
38	34.32316798936688	36.0	36.0	36.0	32.0	36.0
39	34.2096130812089	36.0	36.0	36.0	32.0	36.0
40	34.13759070220313	36.0	36.0	36.0	32.0	36.0
41	34.12977968734303	36.0	36.0	36.0	32.0	36.0
42	34.01228681601459	36.0	36.0	36.0	32.0	36.0
43	34.03099368658573	36.0	36.0	36.0	32.0	36.0
44	34.01633760152078	36.0	36.0	36.0	32.0	36.0
45	33.84745956555673	36.0	36.0	36.0	32.0	36.0
46	33.93935413076572	36.0	36.0	36.0	32.0	36.0
47	33.8689978131013	36.0	36.0	36.0	32.0	36.0
48	33.84242583476937	36.0	36.0	36.0	32.0	36.0
49	33.89188374662886	36.0	36.0	36.0	32.0	36.0
50	33.275624966191934	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	12.0
22	51.0
23	182.0
24	505.0
25	1228.0
26	2565.0
27	4955.0
28	8530.0
29	12536.0
30	18272.0
31	26391.0
32	38764.0
33	57193.0
34	114202.0
35	361640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.719790275510704	17.732446703269524	11.747848345451848	25.799914675767916
2	14.942736588306207	21.104928826447814	37.28211310489791	26.67022148034806
3	19.050651203261655	25.126539140971172	28.59297748337411	27.22983217239306
4	12.108791810013075	16.10703610629426	36.1178856134299	35.66628647026277
5	13.354455323127807	37.589620345112706	34.1513210259105	14.904603305848987
6	34.03196142373615	35.25338856003586	17.190856683615905	13.523793332612088
7	29.167973911766754	30.77886049440911	21.49481867286932	18.558346920954815
8	28.112235041381066	33.45723183444481	20.345267257567208	18.085265866606907
9	27.097606775522188	14.668449156537127	18.458506881389724	39.77543718655096
10	15.20783265202037	27.594797808464765	32.590663565340364	24.606705974174503
11	37.43707836515799	21.141978409205066	21.729272759587968	19.691670466048976
12	23.4658094229833	23.997465361224663	29.80627013994606	22.730455075845974
13	30.46898544900971	19.705270966794686	25.17622694290108	24.649516641294518
14	23.604441799902634	19.723198899595847	24.96140085157681	31.710958448924707
15	25.299713307626327	27.372089608753775	23.730864636379795	23.597332447240102
16	25.812668557342338	25.705719165114715	25.217801200862393	23.26381107668055
17	23.659771109754494	26.150208257667668	26.77629494540481	23.413725687173027
18	25.25875725424436	24.053567426800715	28.364926163190553	22.32274915576437
19	24.856615175376913	27.05634161985055	25.22351959322139	22.863523611551155
20	24.36869721112459	24.3255774417149	27.71503859914842	23.590686748012086
21	25.472810589844446	25.667854134629504	23.9047346743221	24.954600601203953
22	24.023739055847056	26.236756898776726	25.228156127566514	24.5113479178097
23	23.07773149829607	25.25705719165115	25.36014280525783	26.30506850479495
24	23.341086649099353	27.008894418385403	25.370652283106786	24.279366649408455
25	24.971137573701576	24.85336960133532	24.986901790475013	25.188591034488088
26	22.871096617648195	25.67171791325044	26.369361781047395	25.087823688053966
27	25.104978865130946	25.931827490012132	25.71452858037046	23.248665064486467
28	23.975982752092236	24.512275224678724	26.07092352036598	25.44081850286306
29	23.344332223140942	25.928581915970543	26.663472609673356	24.06361325121516
30	24.819677451760725	25.043622060630415	26.554050399128332	23.58265008848053
31	23.651889001367778	25.35813364037494	25.65579914533217	25.334178212925114
32	23.49548324279212	26.186218674414828	25.63431653619974	24.683981546593305
33	23.24186481411361	25.21857395658658	26.29873190785661	25.240829321443197
34	23.338459279637114	26.41279065274676	27.13578090829708	23.112969159319046
35	25.369570425092924	25.50093889820489	26.115588801224042	23.013901875478144
36	23.627160818193758	26.498721089276465	25.947746257930405	23.926371834599365
37	25.113942831531528	25.845897053482425	25.8885531694576	23.15160694552845
38	24.079377467988593	25.850379036682714	25.217028445138208	24.85321505019048
39	24.140579721344285	25.422426916627384	25.96490143500738	24.47209192702095
40	25.562604804995093	25.50402992110164	25.12321590022178	23.810149373681487
41	23.015601938071356	25.683618351402938	26.88478984908081	24.4159898614449
42	26.150826462247018	26.34046071696276	25.374361510582887	22.134351310207332
43	24.571004659717016	24.34582364168863	25.680063675071672	25.403108023522687
44	23.87444264993393	25.495684159280408	26.8157054873384	23.814167703447264
45	24.474719296483187	25.695364238410594	25.855633775607195	23.97428268949902
46	23.622288198592653	25.1194297663334	26.892291428721567	24.36599060635238
47	25.001120495800073	24.41583531030006	27.10100690070862	23.482037293191247
48	24.34644184626798	27.554459959662154	24.55864056813001	23.540457625939865
49	23.233828154582056	25.491974931804307	27.459411005587025	23.814785908026614
50	23.206627153090636	27.62694444659099	24.20255473042417	24.96387366989421
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	9.5
2	14.0
3	63.5
4	113.0
5	114.0
6	115.0
7	111.0
8	107.0
9	150.5
10	194.0
11	288.5
12	383.0
13	627.0
14	871.0
15	1210.5
16	1550.0
17	1811.0
18	2072.0
19	2356.0
20	2640.0
21	3051.5
22	3463.0
23	3833.0
24	4203.0
25	4690.5
26	5178.0
27	6664.5
28	8151.0
29	9425.5
30	10700.0
31	11703.5
32	12707.0
33	14706.0
34	16705.0
35	19171.0
36	21637.0
37	24664.5
38	27692.0
39	28685.0
40	29678.0
41	31616.0
42	33554.0
43	34620.5
44	35687.0
45	42405.5
46	49124.0
47	54721.0
48	60318.0
49	57621.5
50	54925.0
51	48669.5
52	42414.0
53	39998.5
54	37583.0
55	38007.5
56	38432.0
57	37201.0
58	35970.0
59	32676.5
60	29383.0
61	26069.0
62	22755.0
63	19756.5
64	16758.0
65	14146.5
66	11535.0
67	10271.0
68	9007.0
69	8519.5
70	8032.0
71	6279.5
72	4527.0
73	4049.5
74	3572.0
75	2571.0
76	1570.0
77	1428.0
78	1286.0
79	1137.5
80	989.0
81	787.0
82	585.0
83	492.5
84	400.0
85	333.0
86	266.0
87	198.0
88	130.0
89	81.0
90	32.0
91	20.0
92	8.0
93	7.0
94	6.0
95	5.0
96	4.0
97	4.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01406415418022209
2	0.0038637786209401344
3	3.091022896752108E-4
4	1.545511448376054E-4
5	0.0
6	7.727557241880269E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.091022896752108E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	647035.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.862727775852065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26269696611136	49.84332162365588
2	11.001639123978144	13.171762559337344
3	2.670101528473603	4.795186827987054
4	0.9764226283402273	2.33805287978052
5	0.5013569918921548	1.5006298562080067
6	0.28465106261087303	1.0223993443309027
7	0.20589074488681172	0.8627627128908629
8	0.13980765400007644	0.6695414025909672
9	0.10893242023057925	0.5868892636405096
>10	0.6666587264605129	7.962966022375678
>50	0.09118014031589979	3.822974697856224
>100	0.08366808600579621	9.339255483977773
>500	0.005698755083977538	2.2591421456126315
>1k	0.0012951716099948952	1.8251151797556917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4231	0.6539058938079083	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3327	0.5141916588747131	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1602	0.24759093402984383	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1540	0.23800876304991228	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1070	0.16536972497623775	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	991	0.15316018453406693	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	916	0.14156884867124653	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	914	0.14125974638157132	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	844	0.13044116624293894	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	744	0.1149860517591784	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	716	0.11065861970372545	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	714	0.11034951741405025	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	704	0.1088040059656742	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	699	0.10803125024148616	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	693	0.10710394337246053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5455114483760537E-4	0.0
3	0.0	0.0	0.0	1.5455114483760537E-4	0.0
4	0.0	0.0	0.0	1.5455114483760537E-4	0.0
5	0.0	0.0	0.0	1.5455114483760537E-4	0.0
6	0.0	0.0	0.0	1.5455114483760537E-4	0.0
7	0.0	0.0	0.0	1.5455114483760537E-4	0.0
8	0.0	0.0	0.0	1.5455114483760537E-4	0.0
9	0.0	0.0	0.0	1.5455114483760537E-4	0.0
10	0.0	0.0	0.0	1.5455114483760537E-4	0.0
11	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
12	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
13	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
14	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
15	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
16	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
17	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
18	0.0	0.0	0.0	1.5455114483760537E-4	1.5455114483760537E-4
19	0.0	0.0	0.0	4.6365343451281615E-4	1.5455114483760537E-4
20	0.0	0.0	0.0	7.727557241880269E-4	4.6365343451281615E-4
21	0.0	0.0	0.0	7.727557241880269E-4	4.6365343451281615E-4
22	0.0	0.0	0.0	0.001236409158700843	4.6365343451281615E-4
23	0.0	0.0	0.0	0.002163716027726475	4.6365343451281615E-4
24	0.0	0.0	0.0	0.0029364717519145024	4.6365343451281615E-4
25	0.0	0.0	0.0	0.00432743205545295	4.6365343451281615E-4
26	0.0	0.0	0.0	0.00602749464866661	4.6365343451281615E-4
27	0.0	0.0	0.0	0.008809415255743507	4.6365343451281615E-4
28	0.0	0.0	0.0	0.012209540442170826	4.6365343451281615E-4
29	0.0	0.0	0.0	0.017000625932136592	4.6365343451281615E-4
30	0.0	0.0	0.0	0.02812830836044418	4.6365343451281615E-4
31	0.0	0.0	0.0	0.05393834954832428	4.6365343451281615E-4
32	0.0	0.0	0.0	0.0831485159226317	4.6365343451281615E-4
33	0.0	0.0	0.0	0.11962258610430657	4.6365343451281615E-4
34	0.0	0.0	0.0	0.16011498605175917	7.727557241880269E-4
35	0.0	0.0	0.0	0.20848949438592967	7.727557241880269E-4
36	0.0	0.0	0.0	0.2775738561283393	7.727557241880269E-4
37	0.0	0.0	0.0	0.39194170330816724	7.727557241880269E-4
38	0.0	0.0	0.0	0.5217646649717558	9.273068690256323E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGTCG	30	0.005743164	29.33323	9
TAACGGC	95	2.6804628E-8	23.157812	36
TTGACGG	105	3.0631782E-9	23.047537	33
CGCGGGA	125	4.0017767E-11	22.87992	44
TGTAGGA	420	0.0	22.52547	2
TGGTATA	215	0.0	22.511547	44
ATTGACG	120	5.966285E-10	21.999922	32
CGCAATA	255	0.0	21.56855	36
CTAAGAC	75	5.4677446E-5	20.533262	3
GTAGGAC	430	0.0	20.465044	3
ATACGAA	270	0.0	20.370298	40
CCCTATA	65	4.9263035E-4	20.30919	2
CTATACT	65	4.928872E-4	20.307621	4
CTAATCC	65	4.928872E-4	20.307621	3
GTCTTAC	55	0.0044794865	20.001474	1
CCGTCCA	55	0.0044814907	19.99993	9
TCTATAC	55	0.0044814907	19.99993	3
ATCGTTT	155	3.274181E-11	19.870897	29
AAGTACT	190	0.0	19.684141	4
CTGTAGG	430	0.0	19.443295	1
>>END_MODULE