FastQCFastQC Report
Thu 2 Feb 2017
SRR4062606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388657
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG41081.0569731151117823No Hit
TCCATGTACTCTGCGTTGATACCAC34580.8897305336067535No Hit
GTATCAACGCAGAGTACTTTTTTTT28140.7240317297771557No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26590.6841508064951872No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20420.5253990022050291No Hit
TATCAACGCAGAGTACTTTTTTTTT18800.48371700496839115No Hit
GAGTACATGGAAGCAGTGGTATCAA17340.4461517481995693No Hit
CATGTACTCTGCGTTGATACCACTG14380.36999204954497156No Hit
CCATAGGGTCTTCTCGTCTTATTAT14140.3638169388432474No Hit
GTATTAGAGGCACTGCCTGCCCAGT14100.36278775372629335No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13720.35301049511523014No Hit
GTCCTAAAGTGTGTATTTCTCATTT12070.3105566090408767No Hit
CTCTAATACTTGTAATGCTAGAGGT11750.3023231281052445No Hit
GTACATGGGGTGGTATCAACGCAAA11240.28920101786408065No Hit
GTCCTACAGTGGACATTTCTAAATT10830.2786518704153019No Hit
CCATTGGGATGTCCTGATCCAACAT10810.2781372778568249No Hit
GCTTCCATGTACTCTGCGTTGATAC10580.2722194634343393No Hit
GCGTTGATACCACTGCTTCCATGTA10110.2601265383101295No Hit
GGTATCAACGCAGAGTACTTTTTTT9860.25369413132916685No Hit
CTGTAGGACGTGGAATATGGCAAGA9850.25343683504992837No Hit
CTTCTACACCATTGGGATGTCCTGA9770.2513784648160203No Hit
CATGGAAGCAGTGGTATCAACGCAG9280.23877094713333352No Hit
ATCTATAACTTTATAGATGCAACAC8980.23105205875617835No Hit
CTGTTAGTATGAGTAACAAGAATTC8640.2223039852620691No Hit
GTCAGGATACCGCGGCCGTTAAACT8540.21973102246968407No Hit
CTTTAGGACGTGAAATATGGCGAGG8520.21921642991120704No Hit
GATATATTTTGATCAACGGACCAAG8400.216128874560345No Hit
GTTATATAATTTAAGCTCCATAGGG7980.20532243083232773No Hit
GTTATAGATTAACCCAATTTTAAGT7930.2040359494361352No Hit
GTATCAACGCAGAGTACATGGAAGC7860.20223487548146568No Hit
CCCATGTACTCTGCGTTGATACCAC7800.20069109780603464No Hit
ATCCTGACCGTGCAAAGGTAGCATA7620.19605976477974152No Hit
GTATCCTGACCGTGCAAAGGTAGCA7490.19271491314964095No Hit
GGTCAGGATACCGCGGCCGTTAAAC7250.1865398024479168No Hit
GTACATGGGTGGTATCAACGCAAAA7210.18551061733096277No Hit
GTCCTACAGTGTGCATTTCTCATTT6920.1780490252330461No Hit
CACTATAAATAATCCACCTATAACT6750.1736749884859915No Hit
GTTCATGCTAGTCCCTAATTAAGGA6740.173417692206753No Hit
CCTCTAGCATTACAAGTATTAGAGG6700.17238850708979897No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6510.16749987778426736No Hit
CTATAGAACTAGTACCGCAAGGGAA6440.1656988038295978No Hit
GCCTAAAGGAAAGATCCAAAAAGAT6290.16183935964102023No Hit
GTACATGGGAGAAATCGTAAATAGA6280.16158206336178174No Hit
ACGCAGAGTACATGGAAGCAGTGGT6270.16132476708254323No Hit
AAATATATCTGGGTCAATAAGATAT6200.15952369312787368No Hit
TGCTAGAGGTGATGTTTTTGGTAAA6140.15797991545244264No Hit
GATTAAAGATAAGAGACAGTTGGAC6120.15746532289396564No Hit
ATCGTAAATAGATAGAAACCGACCT6040.15540695266005758No Hit
ATAAATAATCCACCTATAACTTCTC5770.1484599531206179No Hit
ATCCTAGCCCTAGCCCTACACAAAT5640.14511510149051735No Hit
ACCTATAACTTCTCTGTTAACCCAA5630.14485780521127883No Hit
AGCATGAACGGCTAAACGAGGGTCC5620.14460050893204032No Hit
CTGTAGGACCTGGAATATGGCGAGA5590.14382862009432482No Hit
CAGTGGTATCAACGCAGAGTACATG5580.1435713238150863No Hit
GACTATAGGCAATAATCACACTATA5550.1427994349773708No Hit
GTTAGTATGAGTAACAAGAATTCCA5520.1420275461396553No Hit
ACTCTGCGTTGATACCACTGCTTCC5480.14099836102270125No Hit
CCTATAACTTCTCTGTTAACCCAAC5450.14022647218498574No Hit
TACTAACAGTGTTGCATCTATAAAG5410.13919728706803172No Hit
GATTAAACCTTGTACCTTTTGCATA5410.13919728706803172No Hit
CCTATAGTCTGATTAACTAACAATG5300.13636702799640815No Hit
ACCCTATGGAGCTTAAATTATATAA5290.13610973171716964No Hit
GATAGAAACCGACCTGGATTGCTCC5200.13379406520402307No Hit
GTATCAACGCAGAGTACATGGGAGA5170.13302217636630756No Hit
GTACATGGGAGTGGTATCAACGCAA5070.13044921357392253No Hit
GTCCTGATCCAACATCGAGGTCGTA5060.130191917294684No Hit
GTGTAGAAGCTATTAATGGTTCGTT4970.12787625078153744No Hit
TCCCAATGGTGTAGAAGCTATTAAT4960.12761895450229896No Hit
GTCCTTCAGTGTGCATTTCTCATTT4940.12710436194382194No Hit
GGATTGCTCCGGTCTGAACTCAGAT4920.12658976938534494No Hit
GTACATGGGAAGCAGTGGTATCAAC4920.12658976938534494No Hit
TATCAACGCAGAGTACATGGAAGCA4910.1263324731061064No Hit
CTGTTAACCCAACACCGGAATGCCT4890.1258178805476294No Hit
CCAATAAAGAAAGCGTTCAAGCTCA4870.12530328798915238No Hit
GTATCAACGCAGAGTACATGGGAAG4860.12504599170991387No Hit
CTATTATATAAATCAAAACATTTAT4790.12324491775524435No Hit
GACCTGGATTGCTCCGGTCTGAACT4730.12170114007981331No Hit
CTGAAGGACCTGGAATATGGCGAGA4720.1214438438005748No Hit
ATTATAACCTAGACTTACAAGTCAA4630.11912817728742825No Hit
GTTATACGCGTATGCCTGGAGAATT4610.11861358472895123No Hit
ATTTAGAAATGTCCACTGTAGGACG4600.11835628844971272No Hit
CTTTATTGGTGGCTGCTTTTAGGCC4570.11758439961199721No Hit
CCATTAATAGCTTCTACACCATTGG4460.11475414054037364No Hit
GGATACCGCGGCCGTTAAACTTTAG4430.11398225170265812No Hit
AGGGATAACAGCGCAATCCTATTTA4410.11346765914418111No Hit
ATTTAAGAGTTCATATCGACAATTA4360.1121811777479886No Hit
GTTATAGGTGGATTATTTATAGTGT4360.1121811777479886No Hit
TTATATAACTTATCTATTTAATTTA4350.11192388146875008No Hit
CTTAAATAGGATTGCGCTGTTATCC4310.11089469635179605No Hit
GTATCAACGCAGAGTACATGGGGTG4300.11063740007255754No Hit
ACCTTTGCACGGTCAGGATACCGCG4270.10986551123484203No Hit
CTATTAAAGGTTTTTTCCGTTCCAG4140.10652065960474146No Hit
CCTTTAGGCATTCCGGTGTTGGGTT4100.10549147448778744No Hit
GCGGTATCCTGACCGTGCAAAGGTA4000.10291851169540236No Hit
CTATAGTATAAGTTTGAAATTTCGG3960.10188932657844835No Hit
CCTTTAATAGTGAATAATTAACAAA3930.10111743774073284No Hit
CAGTTGGACCCTCGTTTAGCCGTTC3930.10111743774073284No Hit
GTTATCCCTAGGGTAACTTGGTCCG3910.10060284518225583No Hit
GTTCTATAGCTCCTAGATGTACGAA3890.1000882526237788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG453.528478E-516.88854615
AGGCGAG350.002160963416.2916818
TAAGGCG350.002162824916.2895816
AGGTCGG350.002168417216.2832855
GTACAGA350.00217589316.27491
TTAGGCA1055.456968E-1215.37667754
GGTAGCT508.6695516E-515.20360858
TAGCTCG508.679088E-515.20164910
TAGTTCT704.41858E-714.9263455
ATTCTTG456.734042E-414.78128517
ACTGTTC1101.0913936E-1114.6853028
TAGGCAT1101.0913936E-1114.6796285
AAGCTCA1058.731149E-1114.47962719
CCTTTAG1058.913048E-1114.4665781
ATGTATG400.00526137414.2533827
CAGGCTG400.00526137414.2533829
TTGCTAA602.555864E-514.25154610
CTGTTCT1152.5465852E-1114.0468129
GGCATTC1152.5465852E-1114.0468127
AAAGCGT1152.5465852E-1114.04500210
Produced by FastQC (version 0.11.5)