##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062606_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388657
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.66934340562501	32.0	32.0	32.0	32.0	32.0
2	30.49973627131378	32.0	32.0	32.0	32.0	32.0
3	30.568686528224116	32.0	32.0	32.0	32.0	32.0
4	30.511340333507437	32.0	32.0	32.0	32.0	32.0
5	30.493365615439835	32.0	32.0	32.0	32.0	32.0
6	33.92989190983309	36.0	36.0	36.0	32.0	36.0
7	33.676220935169056	36.0	36.0	36.0	27.0	36.0
8	33.61575373658521	36.0	36.0	36.0	27.0	36.0
9	33.735872504547714	36.0	36.0	36.0	32.0	36.0
10	33.55013289352823	36.0	36.0	36.0	27.0	36.0
11	33.95943466861526	36.0	36.0	36.0	32.0	36.0
12	33.73161682408911	36.0	36.0	36.0	32.0	36.0
13	33.86385681976653	36.0	36.0	36.0	32.0	36.0
14	33.76078135734079	36.0	36.0	36.0	32.0	36.0
15	33.79228728673355	36.0	36.0	36.0	32.0	36.0
16	33.75490471032298	36.0	36.0	36.0	32.0	36.0
17	33.717784576117246	36.0	36.0	36.0	27.0	36.0
18	33.7831455499322	36.0	36.0	36.0	32.0	36.0
19	33.7066487931518	36.0	36.0	36.0	27.0	36.0
20	33.61092943134949	36.0	36.0	36.0	27.0	36.0
21	33.53863689577185	36.0	36.0	36.0	27.0	36.0
22	33.51890227115425	36.0	36.0	36.0	27.0	36.0
23	33.56067174912584	36.0	36.0	36.0	27.0	36.0
24	33.560877586149225	36.0	36.0	36.0	27.0	36.0
25	33.131030703165	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	11.0
5	34.0
6	67.0
7	17.0
8	40.0
9	82.0
10	41.0
11	17.0
12	33.0
13	27.0
14	228.0
15	225.0
16	286.0
17	414.0
18	543.0
19	803.0
20	1263.0
21	1790.0
22	2534.0
23	3132.0
24	3918.0
25	4681.0
26	6051.0
27	7170.0
28	9138.0
29	11817.0
30	14627.0
31	19530.0
32	27429.0
33	38201.0
34	82969.0
35	151535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.863281732471485	21.74569764090741	12.231359874840463	23.159660751780642
2	12.868879665914475	22.680969424381757	42.72764465074631	21.72250625895745
3	17.489920003293527	29.490712508458966	30.862415442529223	22.156952045718285
4	9.423377799736496	18.700850214097496	41.65431488801054	30.22145709815547
5	9.40635978740654	42.233003461721594	36.95317024206313	11.407466508808731
6	26.18701961935062	41.32110148419281	20.646908317760378	11.844970578696195
7	24.94303743695499	35.57527683161995	23.375633668286078	16.10605206313898
8	24.760317979434856	36.93356372335686	21.88284615800601	16.42327213920227
9	26.36847567712344	16.554338560431834	22.6615194039614	34.41566635848333
10	14.722042621591058	28.054362749770096	36.09440324363033	21.129191385008514
11	30.69783377598941	24.667492729019777	27.442701584545592	17.191971910445222
12	22.753727363368657	28.473011235549784	32.273929969109325	16.499331431972237
13	26.586240474828816	23.807389317382054	29.295536629725234	20.310833578063896
14	19.976041733754105	25.628646873188636	28.959361112900112	25.435950280157144
15	22.18143694682287	32.139655652066395	27.740255324410374	17.93865207670036
16	20.60918281924142	29.820593727863997	30.329834503187588	19.240388949707
17	17.75274299213491	30.39304842453455	31.613902120457222	20.240306462873324
18	18.456532324759944	27.543998412939697	35.9188332015077	18.080636060792653
19	20.924858171925	30.25897471569976	29.926367913104862	18.889799199270378
20	20.26286571024491	29.424008779364375	32.82343636892909	17.489689141461632
21	21.090151179447012	27.941403794428933	28.456155899296142	22.512289126827913
22	19.673609697394497	32.27336798406444	28.73811726446479	19.31490505407627
23	19.03142359500768	31.147904234816394	30.742355378288966	19.078316791886962
24	19.695255958714757	31.996320810864432	30.299977584772076	18.00844564564874
25	20.06425466902662	30.589756867958457	31.024390495255616	18.321597967759303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	397.0
1	397.0
2	657.5
3	918.0
4	918.0
5	918.0
6	2815.0
7	4712.0
8	4712.0
9	4712.0
10	4559.5
11	4407.0
12	4407.0
13	4407.0
14	3889.0
15	3371.0
16	3371.0
17	3371.0
18	5388.5
19	7406.0
20	7406.0
21	7406.0
22	11739.0
23	16072.0
24	16072.0
25	16072.0
26	23828.0
27	31584.0
28	31584.0
29	31584.0
30	37516.5
31	43449.0
32	43449.0
33	43449.0
34	44838.5
35	46228.0
36	46228.0
37	46228.0
38	44284.0
39	42340.0
40	42340.0
41	42340.0
42	41045.5
43	39751.0
44	39751.0
45	39751.0
46	51080.0
47	62409.0
48	62409.0
49	62409.0
50	50768.0
51	39127.0
52	39127.0
53	39127.0
54	29850.5
55	20574.0
56	20574.0
57	20574.0
58	15667.5
59	10761.0
60	10761.0
61	10761.0
62	8621.5
63	6482.0
64	6482.0
65	6482.0
66	5192.5
67	3903.0
68	3903.0
69	3903.0
70	3126.5
71	2350.0
72	2350.0
73	2350.0
74	1834.5
75	1319.0
76	1319.0
77	1319.0
78	962.0
79	605.0
80	605.0
81	605.0
82	422.0
83	239.0
84	239.0
85	239.0
86	148.0
87	57.0
88	57.0
89	57.0
90	37.5
91	18.0
92	18.0
93	18.0
94	16.5
95	15.0
96	15.0
97	15.0
98	89.0
99	163.0
100	163.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008490777214870696
2	0.003602147909339083
3	0.004116740467816095
4	0.012607517682686791
5	0.031390146067097724
6	0.04193929351587646
7	0.06278029213419545
8	0.08490777214870696
9	0.09262666052586213
10	0.11578332565732767
11	0.12092925124209779
12	0.13225028752859205
13	0.1222157326382903
14	0.12401680659295987
15	0.11449684426113513
16	0.11115199263103455
17	0.12504599170991387
18	0.13327947264554607
19	0.13147839869087655
20	0.1219584363590518
21	0.13096380613239952
22	0.15283398986767252
23	0.13919728706803172
24	0.13585243543793113
25	0.13225028752859205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	388657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.736753517844075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40627662278307	24.248871683934777
2	12.791293553688229	8.119082613755822
3	4.126129273690591	3.9284984322563776
4	1.8381797019807509	2.333514244930687
5	1.0040291120200677	1.5932312226460374
6	0.6022298899037241	1.146769294617172
7	0.40278187771811413	0.8948092422215941
8	0.28607109489167304	0.7263174261725448
9	0.21799581729988354	0.6226631569410637
>10	1.6280764541116381	10.671150411208547
>50	0.29903442570421546	6.761575586720907
>100	0.3444163439682698	22.36978165866201
>500	0.038898787083475175	8.33610452327113
>1k	0.01458704515630319	8.247630502661316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4108	1.0569731151117823	No Hit
TCCATGTACTCTGCGTTGATACCAC	3458	0.8897305336067535	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2814	0.7240317297771557	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2659	0.6841508064951872	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2042	0.5253990022050291	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1880	0.48371700496839115	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1734	0.4461517481995693	No Hit
CATGTACTCTGCGTTGATACCACTG	1438	0.36999204954497156	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1414	0.3638169388432474	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1410	0.36278775372629335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1372	0.35301049511523014	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1207	0.3105566090408767	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1175	0.3023231281052445	No Hit
GTACATGGGGTGGTATCAACGCAAA	1124	0.28920101786408065	No Hit
GTCCTACAGTGGACATTTCTAAATT	1083	0.2786518704153019	No Hit
CCATTGGGATGTCCTGATCCAACAT	1081	0.2781372778568249	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1058	0.2722194634343393	No Hit
GCGTTGATACCACTGCTTCCATGTA	1011	0.2601265383101295	No Hit
GGTATCAACGCAGAGTACTTTTTTT	986	0.25369413132916685	No Hit
CTGTAGGACGTGGAATATGGCAAGA	985	0.25343683504992837	No Hit
CTTCTACACCATTGGGATGTCCTGA	977	0.2513784648160203	No Hit
CATGGAAGCAGTGGTATCAACGCAG	928	0.23877094713333352	No Hit
ATCTATAACTTTATAGATGCAACAC	898	0.23105205875617835	No Hit
CTGTTAGTATGAGTAACAAGAATTC	864	0.2223039852620691	No Hit
GTCAGGATACCGCGGCCGTTAAACT	854	0.21973102246968407	No Hit
CTTTAGGACGTGAAATATGGCGAGG	852	0.21921642991120704	No Hit
GATATATTTTGATCAACGGACCAAG	840	0.216128874560345	No Hit
GTTATATAATTTAAGCTCCATAGGG	798	0.20532243083232773	No Hit
GTTATAGATTAACCCAATTTTAAGT	793	0.2040359494361352	No Hit
GTATCAACGCAGAGTACATGGAAGC	786	0.20223487548146568	No Hit
CCCATGTACTCTGCGTTGATACCAC	780	0.20069109780603464	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	762	0.19605976477974152	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	749	0.19271491314964095	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	725	0.1865398024479168	No Hit
GTACATGGGTGGTATCAACGCAAAA	721	0.18551061733096277	No Hit
GTCCTACAGTGTGCATTTCTCATTT	692	0.1780490252330461	No Hit
CACTATAAATAATCCACCTATAACT	675	0.1736749884859915	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	674	0.173417692206753	No Hit
CCTCTAGCATTACAAGTATTAGAGG	670	0.17238850708979897	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	651	0.16749987778426736	No Hit
CTATAGAACTAGTACCGCAAGGGAA	644	0.1656988038295978	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	629	0.16183935964102023	No Hit
GTACATGGGAGAAATCGTAAATAGA	628	0.16158206336178174	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	627	0.16132476708254323	No Hit
AAATATATCTGGGTCAATAAGATAT	620	0.15952369312787368	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	614	0.15797991545244264	No Hit
GATTAAAGATAAGAGACAGTTGGAC	612	0.15746532289396564	No Hit
ATCGTAAATAGATAGAAACCGACCT	604	0.15540695266005758	No Hit
ATAAATAATCCACCTATAACTTCTC	577	0.1484599531206179	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	564	0.14511510149051735	No Hit
ACCTATAACTTCTCTGTTAACCCAA	563	0.14485780521127883	No Hit
AGCATGAACGGCTAAACGAGGGTCC	562	0.14460050893204032	No Hit
CTGTAGGACCTGGAATATGGCGAGA	559	0.14382862009432482	No Hit
CAGTGGTATCAACGCAGAGTACATG	558	0.1435713238150863	No Hit
GACTATAGGCAATAATCACACTATA	555	0.1427994349773708	No Hit
GTTAGTATGAGTAACAAGAATTCCA	552	0.1420275461396553	No Hit
ACTCTGCGTTGATACCACTGCTTCC	548	0.14099836102270125	No Hit
CCTATAACTTCTCTGTTAACCCAAC	545	0.14022647218498574	No Hit
TACTAACAGTGTTGCATCTATAAAG	541	0.13919728706803172	No Hit
GATTAAACCTTGTACCTTTTGCATA	541	0.13919728706803172	No Hit
CCTATAGTCTGATTAACTAACAATG	530	0.13636702799640815	No Hit
ACCCTATGGAGCTTAAATTATATAA	529	0.13610973171716964	No Hit
GATAGAAACCGACCTGGATTGCTCC	520	0.13379406520402307	No Hit
GTATCAACGCAGAGTACATGGGAGA	517	0.13302217636630756	No Hit
GTACATGGGAGTGGTATCAACGCAA	507	0.13044921357392253	No Hit
GTCCTGATCCAACATCGAGGTCGTA	506	0.130191917294684	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	497	0.12787625078153744	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	496	0.12761895450229896	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	494	0.12710436194382194	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	492	0.12658976938534494	No Hit
GTACATGGGAAGCAGTGGTATCAAC	492	0.12658976938534494	No Hit
TATCAACGCAGAGTACATGGAAGCA	491	0.1263324731061064	No Hit
CTGTTAACCCAACACCGGAATGCCT	489	0.1258178805476294	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	487	0.12530328798915238	No Hit
GTATCAACGCAGAGTACATGGGAAG	486	0.12504599170991387	No Hit
CTATTATATAAATCAAAACATTTAT	479	0.12324491775524435	No Hit
GACCTGGATTGCTCCGGTCTGAACT	473	0.12170114007981331	No Hit
CTGAAGGACCTGGAATATGGCGAGA	472	0.1214438438005748	No Hit
ATTATAACCTAGACTTACAAGTCAA	463	0.11912817728742825	No Hit
GTTATACGCGTATGCCTGGAGAATT	461	0.11861358472895123	No Hit
ATTTAGAAATGTCCACTGTAGGACG	460	0.11835628844971272	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	457	0.11758439961199721	No Hit
CCATTAATAGCTTCTACACCATTGG	446	0.11475414054037364	No Hit
GGATACCGCGGCCGTTAAACTTTAG	443	0.11398225170265812	No Hit
AGGGATAACAGCGCAATCCTATTTA	441	0.11346765914418111	No Hit
ATTTAAGAGTTCATATCGACAATTA	436	0.1121811777479886	No Hit
GTTATAGGTGGATTATTTATAGTGT	436	0.1121811777479886	No Hit
TTATATAACTTATCTATTTAATTTA	435	0.11192388146875008	No Hit
CTTAAATAGGATTGCGCTGTTATCC	431	0.11089469635179605	No Hit
GTATCAACGCAGAGTACATGGGGTG	430	0.11063740007255754	No Hit
ACCTTTGCACGGTCAGGATACCGCG	427	0.10986551123484203	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	414	0.10652065960474146	No Hit
CCTTTAGGCATTCCGGTGTTGGGTT	410	0.10549147448778744	No Hit
GCGGTATCCTGACCGTGCAAAGGTA	400	0.10291851169540236	No Hit
CTATAGTATAAGTTTGAAATTTCGG	396	0.10188932657844835	No Hit
CCTTTAATAGTGAATAATTAACAAA	393	0.10111743774073284	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	393	0.10111743774073284	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	391	0.10060284518225583	No Hit
GTTCTATAGCTCCTAGATGTACGAA	389	0.1000882526237788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.572962792385059E-4	0.0
10	0.0	0.0	0.0	2.572962792385059E-4	0.0
11	0.0	0.0	0.0	2.572962792385059E-4	0.0
12	0.0	0.0	0.0	5.145925584770118E-4	0.0
13	0.0	0.0	0.0	5.145925584770118E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGG	45	3.528478E-5	16.888546	15
AGGCGAG	35	0.0021609634	16.29168	18
TAAGGCG	35	0.0021628249	16.28958	16
AGGTCGG	35	0.0021684172	16.283285	5
GTACAGA	35	0.002175893	16.2749	1
TTAGGCA	105	5.456968E-12	15.3766775	4
GGTAGCT	50	8.6695516E-5	15.2036085	8
TAGCTCG	50	8.679088E-5	15.201649	10
TAGTTCT	70	4.41858E-7	14.926345	5
ATTCTTG	45	6.734042E-4	14.781285	17
ACTGTTC	110	1.0913936E-11	14.685302	8
TAGGCAT	110	1.0913936E-11	14.679628	5
AAGCTCA	105	8.731149E-11	14.479627	19
CCTTTAG	105	8.913048E-11	14.466578	1
ATGTATG	40	0.005261374	14.253382	7
CAGGCTG	40	0.005261374	14.253382	9
TTGCTAA	60	2.555864E-5	14.251546	10
CTGTTCT	115	2.5465852E-11	14.046812	9
GGCATTC	115	2.5465852E-11	14.046812	7
AAAGCGT	115	2.5465852E-11	14.045002	10
>>END_MODULE