FastQCFastQC Report
Thu 2 Feb 2017
SRR4062606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388657
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22880.5886938868977015No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19260.49555263381336245No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT14150.3640742351224859No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG13610.35018023604360654No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT11280.2902302029810347No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA10610.2729913522720548No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10400.26758813040804613No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA9120.2346542066655174No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9120.2346542066655174No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9110.2343969103862789No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9070.2333677252693249No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC8760.2253915406129312No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC8720.2243623554959772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.22384776293750017No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA8180.21046835641709785No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC7840.20172028292298866No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT7490.19271491314964095No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7460.19194302431192542No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA7260.1867970987271553No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT6670.17161661825208344No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC6620.17033013685589093No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC6620.17033013685589093No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6570.1690436554596984No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG6490.16698528522579034No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT6450.16595610010883632No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT6320.16261124847873576No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC6190.15926639684863517No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG6100.15695073033548862No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.1556642489392961No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT6030.15514965638081907No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5980.15386317498462657No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA5970.15360587870538805No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5960.15334858242614954No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG5710.1469161754451869No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT5670.14588699032823288No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG5660.14562969404899437No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5610.1443432126528018No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC5550.1427994349773708No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG5480.14099836102270125No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA5420.1394545833472702No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5350.13765350939260065No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT5340.13739621311336217No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5290.13610973171716964No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA5220.13430865776250012No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC5160.13276488008706905No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT5150.13250758380783056No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA5110.13147839869087655No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA5100.13122110241163804No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA5070.13044921357392253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.12890543589849146No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA4810.12375951031372136No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAA4790.12324491775524435No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA4700.12092925124209779No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT4700.12092925124209779No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC4600.11835628844971272No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT4570.11758439961199721No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4540.11681251077428169No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA4490.11552602937808917No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC4430.11398225170265812No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT4420.11372495542341962No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT4370.11243847402722709No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA4320.11115199263103455No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT4310.11089469635179605No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA4280.11012280751408053No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT4230.10883632611788802No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC4210.10832173355941101No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT4210.10832173355941101No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC4180.10754984472169547No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4170.10729254844245696No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.10394769681235641No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA4020.10343310425387937No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA4000.10291851169540236No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA3970.10214662285768686No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA3950.10163203029920985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC207.850495E-443.9994853
GACGGTG250.002350970235.1995933
GGACCTA353.2140978E-431.4282046
CTCTAGG454.048604E-529.3480931
AGGACAG300.005740607629.33298944
CGCTCTC300.005740607629.33298910
TAAGGCT300.005740607629.3329894
GATTACA300.005740607629.33298944
CTCTAAG300.005740607629.3329892
CTAGGAC752.1573214E-929.3329893
TAGGACC2600.028.7688964
GTGCTAA407.002865E-427.5138381
AGATTAT1200.027.4996785
ATACATG801.298722E-724.7624531
TAGCCAA1350.024.44415911
TTATAGC1350.024.4441598
GTAGATC450.001396604724.4441599
TAGGACA1400.023.5711544
TGGGTGG2900.023.5169666
CGCGGTA3950.023.3921343
Produced by FastQC (version 0.11.5)