FastQCFastQC Report
Thu 2 Feb 2017
SRR4062605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486434
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG57271.1773436889691098No Hit
TCCATGTACTCTGCGTTGATACCAC47250.9713547983899152No Hit
GTATCAACGCAGAGTACTTTTTTTT33250.6835459692373478No Hit
GTCCTAAAGTGTGTATTTCTCATTT32340.6648383953424308No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30610.6292734471685778No Hit
GTCCTACAGTGGACATTTCTAAATT29840.6134439615651867No Hit
CTGTAGGACGTGGAATATGGCAAGA28520.5863077005308017No Hit
GTCCTACAGTGTGCATTTCTCATTT26580.5464256199196602No Hit
GAGTACATGGAAGCAGTGGTATCAA25140.5168224260639676No Hit
CTTTAGGACGTGAAATATGGCGAGG25000.5139443377724419No Hit
TATCAACGCAGAGTACTTTTTTTTT24480.5032542955467751No Hit
CATGTACTCTGCGTTGATACCACTG22180.45597141647171047No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21710.4463092629215885No Hit
CTGTAGGACCTGGAATATGGCGAGA18730.38504709785911345No Hit
GTACATGGGGTGGTATCAACGCAAA17110.35174350477145927No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16630.34187577348622833No Hit
GCTTCCATGTACTCTGCGTTGATAC15090.3102168022794459No Hit
CCCATGTACTCTGCGTTGATACCAC14830.3048717811666125No Hit
GTACATGGGAAGCAGTGGTATCAAC14110.29007018423876624No Hit
GCGTTGATACCACTGCTTCCATGTA13350.27444627637048397No Hit
CTGAAGGACCTGGAATATGGCGAGA13180.27095145487363137No Hit
CATGGAAGCAGTGGTATCAACGCAG13120.2697179884629775No Hit
GGTATCAACGCAGAGTACTTTTTTT12400.2549163915351312No Hit
GTCCTTCAGTGTGCATTTCTCATTT11870.2440207715743554No Hit
ATTTAGAAATGTCCACTGTAGGACG11570.23785343952108612No Hit
GATATACACTGTTCTACAAATCCCG11420.23476977349445147No Hit
TTGTAGAACAGTGTATATCAATGAG11420.23476977349445147No Hit
GTGTATATCAATGAGTTACAATGAA10200.20968928981115628No Hit
GTATCAACGCAGAGTACATGGAAGC9310.19139287138645736No Hit
GTACATGGGTGGTATCAACGCAAAA9040.18584227253851499No Hit
GAATATGGCAAGAAAACTGAAAATC9020.18543111706829704No Hit
TTTCTAAATTTTCCACCTTTTTCAG8950.1839920729225342No Hit
ACTCTGCGTTGATACCACTGCTTCC8890.18275860651188033No Hit
GTATCAACGCAGAGTACATGGGAAG8410.17289087522664945No Hit
GTGTATATCAATGAGTTACAATGAG8280.17021836467023274No Hit
ACGCAGAGTACATGGAAGCAGTGGT8220.16898489825957888No Hit
CAGTGGTATCAACGCAGAGTACATG7700.1582948560339121No Hit
GTTCTACAGTGTGGTTTTTATCATT7530.1548000345370595No Hit
GTATCAACGCAGAGTACATGGGGTG7300.15007174662955303No Hit
GGAATATGGCGAGAAAACTGAAAAT7080.14554903645715556No Hit
GTACATGGGAGTGGTATCAACGCAA7030.14452114778161065No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6920.14225979269541192No Hit
CTGTAGGACATGGAATATGGCAAGA6750.1387649711985593No Hit
TATCAACGCAGAGTACATGGAAGCA6580.1352701497017067No Hit
ACCTGGAATATGGCGAGAAAACTGA6450.13259763914529No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6360.13074743952930923No Hit
GACCTGGAATATGGCGAGAAAACTG6060.12458010747603993No Hit
GATATACACTGTTCTACAATGCCGG6040.12416895200582197No Hit
CCATATTCCAGGTCCTTCAGTGTGC6000.12334664106538606No Hit
GAAATATGGCGAGGAAAACTGAAAA5660.11635699807168084No Hit
GCCATATTCCACGTCCTACAGTGGA5480.11265659883971926No Hit
GGTATCAACGCAGAGTACATGGGAA5430.11162871016417437No Hit
GTTCTACAAATCCCGTTTCCAACGA5340.10977851054819358No Hit
TTCCAGGTCCTTCAGTGTGCATTTC5230.10751715546199485No Hit
GTCCACTGTAGGACGTGGAATATGG5130.10546137811090507No Hit
CCATATTTCACGTCCTAAAGTGTGT5100.10484464490557814No Hit
ACAGTGGACATTTCTAAATTTTCCA5040.1036111784949243No Hit
AGTGTGTATTTCTCATTTTCCGTGA5010.10299444528959736No Hit
GCGTTGATACCACTGCTTCCCATGT4960.10196655661405248No Hit
TATCAACGCAGAGTACATGGGGTGG4950.10176097887894349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCC250.00602511219.0006169
ATGCAAC402.7533734E-416.6272517
TTGTTAC350.002165353216.2879215
GCGAGGT350.002165353216.2879215
CTACCCT350.00216833316.2845694
ATATTAC755.8220394E-815.198933
TGCAATA456.748839E-414.77825811
AACCTTG456.7541085E-414.7767376
TAGGACC6600.014.3929254
ACCCTGG400.005274660414.2489976
AAATGTC3850.013.8172087
TAGAAAT4200.013.7966484
GAAATGT4250.013.6343346
CACCTTT2800.013.5718714
CCACCTT2850.013.33376713
CTCATAT500.001495434413.30180119
CTTACAC500.001497756513.2990653
ACTTTAT655.4410506E-513.152928
GAGGCAC655.4410506E-513.152927
TGTCCAC3950.012.98776310
Produced by FastQC (version 0.11.5)