##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062605_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486434
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.882637726803637	32.0	32.0	32.0	32.0	32.0
2	30.792666631033192	32.0	32.0	32.0	32.0	32.0
3	30.79630124538992	32.0	32.0	32.0	32.0	32.0
4	30.758639404317954	32.0	32.0	32.0	32.0	32.0
5	30.77651027683098	32.0	32.0	32.0	32.0	32.0
6	34.307729311684625	36.0	36.0	36.0	32.0	36.0
7	34.064471233507525	36.0	36.0	36.0	32.0	36.0
8	34.04986287965068	36.0	36.0	36.0	32.0	36.0
9	34.10019242076006	36.0	36.0	36.0	32.0	36.0
10	33.9482170243034	36.0	36.0	36.0	32.0	36.0
11	34.2498530119194	36.0	36.0	36.0	32.0	36.0
12	34.076180941299334	36.0	36.0	36.0	32.0	36.0
13	34.14494052636123	36.0	36.0	36.0	32.0	36.0
14	34.11141285354231	36.0	36.0	36.0	32.0	36.0
15	34.117220424559136	36.0	36.0	36.0	32.0	36.0
16	34.101477281604495	36.0	36.0	36.0	32.0	36.0
17	34.046686703643246	36.0	36.0	36.0	32.0	36.0
18	34.12279980429	36.0	36.0	36.0	32.0	36.0
19	33.992373066027454	36.0	36.0	36.0	32.0	36.0
20	33.89845076618822	36.0	36.0	36.0	32.0	36.0
21	33.78610458972852	36.0	36.0	36.0	32.0	36.0
22	33.765587931764635	36.0	36.0	36.0	32.0	36.0
23	33.80199780442979	36.0	36.0	36.0	32.0	36.0
24	33.75874424896286	36.0	36.0	36.0	32.0	36.0
25	33.30747028373839	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	19.0
5	50.0
6	76.0
7	29.0
8	87.0
9	92.0
10	49.0
11	26.0
12	38.0
13	22.0
14	211.0
15	225.0
16	337.0
17	383.0
18	626.0
19	829.0
20	1180.0
21	1633.0
22	2197.0
23	3093.0
24	3558.0
25	4730.0
26	5969.0
27	7516.0
28	9890.0
29	12539.0
30	16322.0
31	22169.0
32	31617.0
33	46208.0
34	103758.0
35	210952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4295729764181	19.079545220913257	12.30802442484426	24.18285737782438
2	11.160497685090006	21.618504477685583	46.58252674687302	20.63847109035139
3	20.041117575707737	28.05452190538846	29.149071770728398	22.755288748175406
4	10.484869170446462	17.529062953626752	38.850805373819284	33.1352625021075
5	10.013696545426875	41.516984950252336	36.30427718846529	12.165041315855499
6	29.092913528344656	37.97023410408191	20.039531545021124	12.897320822552313
7	25.73939663259764	32.589616353132875	22.976637206010633	18.69434980825886
8	23.89825032871926	40.93650522555594	20.993380345160368	14.171864100564429
9	27.333133040851436	15.138613372404086	20.77348566536888	36.754767921375596
10	15.299558900111357	29.469506886139417	35.53643139280943	19.694502820939793
11	33.32955974583859	21.309791636220677	27.20502724187118	18.155621376069554
12	24.913746963646094	24.768207830705258	33.91946971880275	16.3985754868459
13	31.85602637910913	23.114241549155174	26.422950812923624	18.606781258812074
14	20.0328518763238	24.796168104450853	27.842994378541714	27.327985640683632
15	21.381937783778664	36.26933193922454	25.59554928023645	16.753180996760346
16	18.778788090837615	27.498672506267006	35.05818332997172	18.664356072923656
17	17.885209931127605	30.643687246051055	32.368790472309016	19.102312350512324
18	18.945851645241305	26.082195164627052	38.793524017332416	16.178429172799223
19	22.854390237375224	27.664681506306472	28.179533154511077	21.30139510180723
20	22.448290695927266	27.8035406645384	33.04908395956702	16.699084679967314
21	20.908048556060347	27.148618021404353	26.891304661077136	25.05202876145816
22	21.2089704047471	32.8442656366459	27.803560324983636	18.14320363362337
23	18.274535961921703	31.98671706441359	30.321409160531648	19.41733781313306
24	21.916344675253562	29.26762993048279	31.35067592464886	17.465349469614782
25	21.007550556616824	30.023343323891705	30.815863579474346	18.153242540017125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	241.0
2	542.0
3	843.0
4	843.0
5	843.0
6	2468.0
7	4093.0
8	4093.0
9	4093.0
10	3844.5
11	3596.0
12	3596.0
13	3596.0
14	2895.5
15	2195.0
16	2195.0
17	2195.0
18	3807.0
19	5419.0
20	5419.0
21	5419.0
22	9783.0
23	14147.0
24	14147.0
25	14147.0
26	23011.5
27	31876.0
28	31876.0
29	31876.0
30	43769.0
31	55662.0
32	55662.0
33	55662.0
34	56658.5
35	57655.0
36	57655.0
37	57655.0
38	56849.0
39	56043.0
40	56043.0
41	56043.0
42	58195.0
43	60347.0
44	60347.0
45	60347.0
46	72332.0
47	84317.0
48	84317.0
49	84317.0
50	68216.0
51	52115.0
52	52115.0
53	52115.0
54	37880.5
55	23646.0
56	23646.0
57	23646.0
58	18878.0
59	14110.0
60	14110.0
61	14110.0
62	11366.0
63	8622.0
64	8622.0
65	8622.0
66	6926.5
67	5231.0
68	5231.0
69	5231.0
70	4149.5
71	3068.0
72	3068.0
73	3068.0
74	2413.5
75	1759.0
76	1759.0
77	1759.0
78	1260.5
79	762.0
80	762.0
81	762.0
82	529.5
83	297.0
84	297.0
85	297.0
86	204.0
87	111.0
88	111.0
89	111.0
90	68.0
91	25.0
92	25.0
93	25.0
94	23.0
95	21.0
96	21.0
97	21.0
98	127.0
99	233.0
100	233.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009045420344794977
2	0.004522710172397489
3	0.004933865642615442
4	0.015623907868282235
5	0.03700399231961581
6	0.049338656426154426
7	0.07441914010944958
8	0.09394902494480238
9	0.0982661573820909
10	0.123963374270713
11	0.12355221880049504
12	0.1344478387612708
13	0.12231875238984118
14	0.12684146256223866
15	0.11923508636320651
16	0.11430122072059107
17	0.12499126294625788
18	0.13136417273463616
19	0.1362980383772516
20	0.12190759691962322
21	0.13259763914529
22	0.1537721458615146
23	0.14349325910606578
24	0.13465341649637977
25	0.13198090593996306
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	486434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.78911078535524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1521648293092	29.15085647323352
2	13.79778313619541	10.7040747732438
3	4.500289396054103	5.236866718491061
4	1.936643373240598	3.004826974254146
5	1.0675871020087884	2.070537718641762
6	0.6470507733059438	1.5059114477708422
7	0.4197421018587006	1.13969960231925
8	0.3022190180381864	0.937824557769959
9	0.22695140097640332	0.7922918731828759
>10	1.5758539944246517	11.890385670947493
>50	0.1950738636147481	5.32488885837619
>100	0.147895673773137	11.493428293495487
>500	0.01590276062076742	4.222159625358004
>1k	0.014312484558690676	11.348673234751356
>5k	5.300920206922472E-4	1.1775741781642783
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5727	1.1773436889691098	No Hit
TCCATGTACTCTGCGTTGATACCAC	4725	0.9713547983899152	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3325	0.6835459692373478	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3234	0.6648383953424308	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3061	0.6292734471685778	No Hit
GTCCTACAGTGGACATTTCTAAATT	2984	0.6134439615651867	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2852	0.5863077005308017	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2658	0.5464256199196602	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2514	0.5168224260639676	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2500	0.5139443377724419	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2448	0.5032542955467751	No Hit
CATGTACTCTGCGTTGATACCACTG	2218	0.45597141647171047	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2171	0.4463092629215885	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1873	0.38504709785911345	No Hit
GTACATGGGGTGGTATCAACGCAAA	1711	0.35174350477145927	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1663	0.34187577348622833	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1509	0.3102168022794459	No Hit
CCCATGTACTCTGCGTTGATACCAC	1483	0.3048717811666125	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1411	0.29007018423876624	No Hit
GCGTTGATACCACTGCTTCCATGTA	1335	0.27444627637048397	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1318	0.27095145487363137	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1312	0.2697179884629775	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1240	0.2549163915351312	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1187	0.2440207715743554	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1157	0.23785343952108612	No Hit
GATATACACTGTTCTACAAATCCCG	1142	0.23476977349445147	No Hit
TTGTAGAACAGTGTATATCAATGAG	1142	0.23476977349445147	No Hit
GTGTATATCAATGAGTTACAATGAA	1020	0.20968928981115628	No Hit
GTATCAACGCAGAGTACATGGAAGC	931	0.19139287138645736	No Hit
GTACATGGGTGGTATCAACGCAAAA	904	0.18584227253851499	No Hit
GAATATGGCAAGAAAACTGAAAATC	902	0.18543111706829704	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	895	0.1839920729225342	No Hit
ACTCTGCGTTGATACCACTGCTTCC	889	0.18275860651188033	No Hit
GTATCAACGCAGAGTACATGGGAAG	841	0.17289087522664945	No Hit
GTGTATATCAATGAGTTACAATGAG	828	0.17021836467023274	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	822	0.16898489825957888	No Hit
CAGTGGTATCAACGCAGAGTACATG	770	0.1582948560339121	No Hit
GTTCTACAGTGTGGTTTTTATCATT	753	0.1548000345370595	No Hit
GTATCAACGCAGAGTACATGGGGTG	730	0.15007174662955303	No Hit
GGAATATGGCGAGAAAACTGAAAAT	708	0.14554903645715556	No Hit
GTACATGGGAGTGGTATCAACGCAA	703	0.14452114778161065	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	692	0.14225979269541192	No Hit
CTGTAGGACATGGAATATGGCAAGA	675	0.1387649711985593	No Hit
TATCAACGCAGAGTACATGGAAGCA	658	0.1352701497017067	No Hit
ACCTGGAATATGGCGAGAAAACTGA	645	0.13259763914529	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	636	0.13074743952930923	No Hit
GACCTGGAATATGGCGAGAAAACTG	606	0.12458010747603993	No Hit
GATATACACTGTTCTACAATGCCGG	604	0.12416895200582197	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	600	0.12334664106538606	No Hit
GAAATATGGCGAGGAAAACTGAAAA	566	0.11635699807168084	No Hit
GCCATATTCCACGTCCTACAGTGGA	548	0.11265659883971926	No Hit
GGTATCAACGCAGAGTACATGGGAA	543	0.11162871016417437	No Hit
GTTCTACAAATCCCGTTTCCAACGA	534	0.10977851054819358	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	523	0.10751715546199485	No Hit
GTCCACTGTAGGACGTGGAATATGG	513	0.10546137811090507	No Hit
CCATATTTCACGTCCTAAAGTGTGT	510	0.10484464490557814	No Hit
ACAGTGGACATTTCTAAATTTTCCA	504	0.1036111784949243	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	501	0.10299444528959736	No Hit
GCGTTGATACCACTGCTTCCCATGT	496	0.10196655661405248	No Hit
TATCAACGCAGAGTACATGGGGTGG	495	0.10176097887894349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.0557773510897676E-4	0.0
5	0.0	0.0	0.0	2.0557773510897676E-4	0.0
6	0.0	0.0	0.0	4.111554702179535E-4	0.0
7	0.0	0.0	0.0	4.111554702179535E-4	0.0
8	0.0	0.0	0.0	4.111554702179535E-4	0.0
9	0.0	0.0	0.0	4.111554702179535E-4	0.0
10	0.0	0.0	0.0	4.111554702179535E-4	0.0
11	0.0	0.0	0.0	4.111554702179535E-4	0.0
12	0.0	0.0	0.0	4.111554702179535E-4	0.0
13	0.0	0.0	0.0	4.111554702179535E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCCC	25	0.006025112	19.000616	9
ATGCAAC	40	2.7533734E-4	16.62725	17
TTGTTAC	35	0.0021653532	16.28792	15
GCGAGGT	35	0.0021653532	16.28792	15
CTACCCT	35	0.002168333	16.284569	4
ATATTAC	75	5.8220394E-8	15.19893	3
TGCAATA	45	6.748839E-4	14.778258	11
AACCTTG	45	6.7541085E-4	14.776737	6
TAGGACC	660	0.0	14.392925	4
ACCCTGG	40	0.0052746604	14.248997	6
AAATGTC	385	0.0	13.817208	7
TAGAAAT	420	0.0	13.796648	4
GAAATGT	425	0.0	13.634334	6
CACCTTT	280	0.0	13.57187	14
CCACCTT	285	0.0	13.333767	13
CTCATAT	50	0.0014954344	13.301801	19
CTTACAC	50	0.0014977565	13.299065	3
ACTTTAT	65	5.4410506E-5	13.15292	8
GAGGCAC	65	5.4410506E-5	13.15292	7
TGTCCAC	395	0.0	12.987763	10
>>END_MODULE