FastQCFastQC Report
Thu 2 Feb 2017
SRR4062605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486434
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22470.46193317078987073No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20910.4298630441128704No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15270.3139172015114075No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14550.2991156045835612No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA13400.2754741650460289No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC11570.23785343952108612No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA11340.23312515161357963No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8580.17638569672350207No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8290.17042394240534173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.1698072092000148No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7000.14390441457628372No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA6980.14349325910606578No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG6230.12807492897289252No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA5790.11902950862809755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.11697373127700778No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5630.11574026486635391No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5590.114917953925918No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA5500.11306775430993722No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC5500.11306775430993722No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5430.11162871016417437No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG5420.1114231324290654No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5400.11101197695884744No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA5250.1079283109322128No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA5240.10772273319710382No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA5200.10690042225666792No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5180.10648926678644996No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA5120.1052558003757961No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5090.10463906717046918No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC5000.10278886755448838No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA4990.1025832898193794No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4880.10032193473318066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG250.002351469435.19983718
AGTTGCC250.002351469435.1998378
ACGCTTA353.215145E-431.42842513
ACGTTAT300.005741812829.33319929
TAGCACC300.005741812829.3331994
AGCGAGA300.005741812829.33319919
TATAGCG300.005741812829.33319916
CCGGTTA300.005741812829.33319934
GCGCCAT407.026241E-427.49987221
GTATGGG407.026241E-427.4998722
GCTAGTT407.026241E-427.49987239
TCCGTCA407.026241E-427.49987244
GCGTATG407.026241E-427.4998728
TAGGACC7150.025.8460354
GTGATTC450.001397052424.44432839
TTAACAC450.001397052424.4443283
CACGTTA450.001397052424.44432828
CCCGGGA450.001397052424.44432843
ATAGGAC2150.023.5347753
GATATAC5250.023.4713841
Produced by FastQC (version 0.11.5)