FastQCFastQC Report
Thu 2 Feb 2017
SRR4062604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301889
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG41600.31953569006267046No Hit
TCCATGTACTCTGCGTTGATACCAC36070.2770589504942434No Hit
GTATCAACGCAGAGTACTTTTTTTT23200.1782025963811047No Hit
GAGTACATGGAAGCAGTGGTATCAA19660.15101133814019477No Hit
GCGCAAGACGGACCAGAGCGAAAGC18260.14025773318616258No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17760.13641715998829393No Hit
CATGTACTCTGCGTTGATACCACTG17260.1325765867904253No Hit
GAATAACGCCGCCGCATCGCCAGTC17030.13080992311940573No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16860.1295041282321304No Hit
GAATAGGACCGCGGTTCTATTTTGT16460.12643166967383548No Hit
GAACTACGACGGTATCTGATCGTCT15970.12266790793992423No Hit
GTATCTGATCGTCTTCGAACCTCCG15500.1190577691339277No Hit
GTCCTATTCCATTATTCCTAGCTGC15430.11852008888622609No Hit
TATCAACGCAGAGTACTTTTTTTTT15320.11767516278269499No Hit
GATTAAGAGGGACGGCCGGGGGCAT13700.10523170562160061No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13460.10338823048662367No Hit
GGGTAGGCACACGCTGAGCCAGTCA13410.1030041731668368No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG13130.10085345217603038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT402.7594005E-416.626016
TATACCG551.1294333E-515.5434075
ATCGCCA2250.014.78208816
CGCATCG2250.014.77981313
CGAACGA1203.6379788E-1214.25415616
CGGTTCT2800.014.25141312
ATAGCGA400.00527635914.2508666
GCACCGT602.5661515E-514.2508656
GCATCGC2450.013.57382114
GGTTCTA2800.013.573297513
CGCCAGT2400.013.46381318
TCGCCAG2450.013.18802617
CGTTATT802.0155167E-613.0527482
CCGCATC2550.013.04050912
GTCCTAA2000.012.8149311
ACCGGTC604.0878012E-412.6679248
GAGCGTT604.0891286E-412.6674367
CCTATAC604.107749E-412.6606173
CATCGCC2600.012.42526615
CTTACAC1155.356924E-912.3853853
Produced by FastQC (version 0.11.5)