##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062604_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1301889
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22237610118835	32.0	32.0	32.0	32.0	32.0
2	30.93642699185568	32.0	32.0	32.0	32.0	32.0
3	30.920328845239496	32.0	32.0	32.0	32.0	32.0
4	30.99336733008728	32.0	32.0	32.0	32.0	32.0
5	30.80791296339396	32.0	32.0	32.0	32.0	32.0
6	34.44016041306133	36.0	36.0	36.0	32.0	36.0
7	34.33636969050357	36.0	36.0	36.0	32.0	36.0
8	34.3494952334646	36.0	36.0	36.0	32.0	36.0
9	34.49308504795724	36.0	36.0	36.0	32.0	36.0
10	34.20749541627589	36.0	36.0	36.0	32.0	36.0
11	34.468622901030734	36.0	36.0	36.0	32.0	36.0
12	34.265598680071804	36.0	36.0	36.0	32.0	36.0
13	34.3825187861638	36.0	36.0	36.0	32.0	36.0
14	34.30137822809779	36.0	36.0	36.0	32.0	36.0
15	34.23499161602871	36.0	36.0	36.0	32.0	36.0
16	34.24113730125994	36.0	36.0	36.0	32.0	36.0
17	34.1653182414169	36.0	36.0	36.0	32.0	36.0
18	34.18157922833667	36.0	36.0	36.0	32.0	36.0
19	34.169732596250526	36.0	36.0	36.0	32.0	36.0
20	34.16183868209963	36.0	36.0	36.0	32.0	36.0
21	34.12707304539788	36.0	36.0	36.0	32.0	36.0
22	34.08784312641093	36.0	36.0	36.0	32.0	36.0
23	34.04562370524676	36.0	36.0	36.0	32.0	36.0
24	34.01083809756439	36.0	36.0	36.0	32.0	36.0
25	33.55622868001804	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	46.0
5	122.0
6	205.0
7	63.0
8	235.0
9	242.0
10	141.0
11	51.0
12	96.0
13	72.0
14	205.0
15	295.0
16	406.0
17	537.0
18	751.0
19	933.0
20	1422.0
21	2083.0
22	2994.0
23	4499.0
24	6438.0
25	9043.0
26	13072.0
27	17234.0
28	24156.0
29	33133.0
30	43962.0
31	62364.0
32	91229.0
33	133649.0
34	302389.0
35	549817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64721914736548	17.472731504704722	10.990438454512562	25.88961089341723
2	16.701040905728554	19.88897312817866	36.99874101949	26.411244946602785
3	19.414239054621653	23.442267334855295	28.445462841481362	28.698030769041686
4	12.396115818666503	14.935583741136524	36.04498766987532	36.62331277032166
5	14.386459910913995	36.49345071109749	33.8345477582891	15.285541619699424
6	34.54781447955803	35.269571472155256	16.681562382566558	13.501051665720155
7	30.21715452322626	30.55721031266839	20.611039668307246	18.61459549579811
8	28.13577773438281	33.014098410565275	19.711481540138724	19.13864231491319
9	26.998072488872793	14.26627332215423	18.817721415824966	39.91793277314801
10	15.666477228595358	26.501591510508433	31.804216906847994	26.02771435404822
11	37.55769653736182	21.076750814019125	22.20961011675426	19.155942531864792
12	24.21500106525269	23.84112429153005	28.95610620530842	22.987768437908844
13	29.333657355639602	19.17748065479166	25.67617866959107	25.81268331997767
14	23.184675265347305	20.11263753800152	24.838553631264723	31.864133565386453
15	25.057636200609352	27.66852917337615	22.43643869030866	24.83739593570584
16	25.752359449988425	25.813570961136072	24.308044622884033	24.12602496599147
17	23.631738062772587	26.112970965506687	25.824956297907914	24.430334673812816
18	24.865404494105487	24.46192562329256	27.148759567817677	23.523910314784278
19	25.22234058508859	25.590684116002443	25.237185083202256	23.94979021570671
20	25.058794214864594	24.540528401950937	25.76155619711789	24.63912118606658
21	26.229655717165535	24.679563845341534	24.198938455491966	24.89184198200097
22	25.0662539242709	25.169260025401503	25.013943133554832	24.750542916772762
23	23.648398138533132	24.618591592631052	25.764855197877008	25.968155070958808
24	24.150645894464805	25.78355324639364	25.405669411253445	24.660131447888105
25	24.597210856309566	24.913473785248858	25.425708976575624	25.06360638186595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	367.5
3	606.0
4	606.0
5	606.0
6	1702.5
7	2799.0
8	2799.0
9	2799.0
10	2972.5
11	3146.0
12	3146.0
13	3146.0
14	3627.0
15	4108.0
16	4108.0
17	4108.0
18	6513.0
19	8918.0
20	8918.0
21	8918.0
22	14667.0
23	20416.0
24	20416.0
25	20416.0
26	30600.0
27	40784.0
28	40784.0
29	40784.0
30	50768.5
31	60753.0
32	60753.0
33	60753.0
34	73241.0
35	85729.0
36	85729.0
37	85729.0
38	95496.5
39	105264.0
40	105264.0
41	105264.0
42	119088.5
43	132913.0
44	132913.0
45	132913.0
46	156323.5
47	179734.0
48	179734.0
49	179734.0
50	175229.0
51	170724.0
52	170724.0
53	170724.0
54	155601.0
55	140478.0
56	140478.0
57	140478.0
58	129468.0
59	118458.0
60	118458.0
61	118458.0
62	105940.5
63	93423.0
64	93423.0
65	93423.0
66	77610.5
67	61798.0
68	61798.0
69	61798.0
70	46910.5
71	32023.0
72	32023.0
73	32023.0
74	25124.5
75	18226.0
76	18226.0
77	18226.0
78	15331.5
79	12437.0
80	12437.0
81	12437.0
82	8755.0
83	5073.0
84	5073.0
85	5073.0
86	3725.0
87	2377.0
88	2377.0
89	2377.0
90	1630.5
91	884.0
92	884.0
93	884.0
94	506.5
95	129.0
96	129.0
97	129.0
98	344.5
99	560.0
100	560.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008602883963225744
2	0.003226081486209654
3	0.004685499301399735
4	0.01528548132751717
5	0.034565158780817715
6	0.04762310765357108
7	0.07112741562452712
8	0.09025347014991293
9	0.09586070701880114
10	0.11921139206184245
11	0.11913458059788508
12	0.13273020971834004
13	0.12036356402120303
14	0.12382007989928481
15	0.11452589276044273
16	0.1136809666569116
17	0.12351283404345532
18	0.1303490543356615
19	0.13395919314165802
20	0.12220703915617998
21	0.1311939804391926
22	0.15101133814019477
23	0.14125628221760841
24	0.13472730778123174
25	0.13096354604732047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1301889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.70011325336118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28032249013246	40.39641233029304
2	11.124123293388584	11.057403750515316
3	3.074659511447314	4.5843277780336695
4	1.2528590085567515	2.490689384630574
5	0.707047098285007	1.757016043011262
6	0.4594586603162969	1.3701088471774532
7	0.31351811719857503	1.090732015222482
8	0.25095377732545576	0.9977944923547147
9	0.18293659843716106	0.8182772694460404
>10	1.1042497753859202	10.301381784871214
>50	0.11311386482096714	3.9588502396587697
>100	0.1160040782227917	12.165641136298563
>500	0.015952491251738535	5.2647696285847685
>1k	0.004801235231105773	3.746595299902135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4160	0.31953569006267046	No Hit
TCCATGTACTCTGCGTTGATACCAC	3607	0.2770589504942434	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2320	0.1782025963811047	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1966	0.15101133814019477	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1826	0.14025773318616258	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1776	0.13641715998829393	No Hit
CATGTACTCTGCGTTGATACCACTG	1726	0.1325765867904253	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1703	0.13080992311940573	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1686	0.1295041282321304	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1646	0.12643166967383548	No Hit
GAACTACGACGGTATCTGATCGTCT	1597	0.12266790793992423	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1550	0.1190577691339277	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1543	0.11852008888622609	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1532	0.11767516278269499	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1370	0.10523170562160061	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1346	0.10338823048662367	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1341	0.1030041731668368	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1313	0.10085345217603038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.68114639573727E-5
7	0.0	0.0	0.0	0.0	7.68114639573727E-5
8	0.0	0.0	0.0	7.68114639573727E-5	7.68114639573727E-5
9	0.0	0.0	0.0	2.3043439187211813E-4	7.68114639573727E-5
10	0.0	0.0	0.0	2.3043439187211813E-4	7.68114639573727E-5
11	0.0	0.0	0.0	2.3043439187211813E-4	7.68114639573727E-5
12	0.0	0.0	0.0	2.3043439187211813E-4	1.536229279147454E-4
13	0.0	0.0	0.0	2.3043439187211813E-4	2.3043439187211813E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	40	2.7594005E-4	16.62601	6
TATACCG	55	1.1294333E-5	15.543407	5
ATCGCCA	225	0.0	14.782088	16
CGCATCG	225	0.0	14.779813	13
CGAACGA	120	3.6379788E-12	14.254156	16
CGGTTCT	280	0.0	14.251413	12
ATAGCGA	40	0.005276359	14.250866	6
GCACCGT	60	2.5661515E-5	14.250865	6
GCATCGC	245	0.0	13.573821	14
GGTTCTA	280	0.0	13.5732975	13
CGCCAGT	240	0.0	13.463813	18
TCGCCAG	245	0.0	13.188026	17
CGTTATT	80	2.0155167E-6	13.052748	2
CCGCATC	255	0.0	13.040509	12
GTCCTAA	200	0.0	12.814931	1
ACCGGTC	60	4.0878012E-4	12.667924	8
GAGCGTT	60	4.0891286E-4	12.667436	7
CCTATAC	60	4.107749E-4	12.660617	3
CATCGCC	260	0.0	12.425266	15
CTTACAC	115	5.356924E-9	12.385385	3
>>END_MODULE