FastQCFastQC Report
Thu 2 Feb 2017
SRR4062604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301889
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19570.1503200349645784No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19390.1489374286133457No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17130.13157803775897947No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17050.13096354604732047No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16210.12451138307490116No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15740.12090124426890464No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15100.1159853105756328No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14770.1134505322650395No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14710.11298966348129527No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC13790.10592300879721697No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13200.10139113242373199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT2500.021.99982642
TTAACGG1850.021.40523735
CGCAATA5450.020.9906636
TAACGGC1950.020.30753336
ATACGAA5550.020.21605940
GATTTCG2650.019.92437241
TCTAGCG5600.019.64270228
CTAGCGG5650.019.46887429
CGGTCCA5800.019.34467710
GTCCTAC6500.019.2966041
GTGTAGG1950.019.1837561
TAGCGGC5850.019.17933730
TCCTACA6450.019.1028272
CGAATGC5900.019.016843
CCGCAAT708.1242097E-418.85699522
TTGAACG951.5973712E-518.5261715
CAATACG6150.018.2437638
GTCCTAA3150.018.1627731
AATACGA6300.018.15858739
TGGTATA1701.2732926E-1018.11750444
Produced by FastQC (version 0.11.5)