FastQCFastQC Report
Thu 2 Feb 2017
SRR4062603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18529
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT2031.095579901775595No Hit
TATCAACGCAGAGTACTTTTTTTTT1440.7771601273679098No Hit
GGTATCAACGCAGAGTACTTTTTTT680.3669922823681796No Hit
GTACTTTTTTTTTTTTTTTTTTTTT600.32381671973662907No Hit
ACGCAGAGTACTTTTTTTTTTTTTT540.29143504776296614No Hit
GAGTACTTTTTTTTTTTTTTTTTTT530.28603810243402233No Hit
GTACATGGAAGCAGTGGTATCAACG390.2104808678288089No Hit
TCCATGTACTCTGCGTTGATACCAC360.19429003184197746No Hit
GAGTACATGGAAGCAGTGGTATCAA310.16730530519725834No Hit
GTATCAACGCAGAGTACATGGGCAG260.14032057855253927No Hit
GTTCTACAGTGTGGTTTTTATCATT250.13492363322359543No Hit
GATATACACTGTTCTACAAATCCCG220.118732797236764No Hit
GTCCTAAAGTGTGTATTTCTCATTT200.10793890657887636No Hit
ATCAACGCAGAGTACTTTTTTTTTT200.10793890657887636No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)