##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062603_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18529
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.69269793296994	32.0	32.0	32.0	14.0	32.0
2	27.440444708295104	32.0	21.0	32.0	14.0	32.0
3	27.735279831615305	32.0	21.0	32.0	14.0	32.0
4	27.301851152247828	32.0	21.0	32.0	14.0	32.0
5	27.52242430784176	32.0	21.0	32.0	14.0	32.0
6	30.854552323384965	36.0	27.0	36.0	14.0	36.0
7	30.692158238437045	36.0	21.0	36.0	14.0	36.0
8	30.371201899724756	36.0	21.0	36.0	14.0	36.0
9	30.701710831669274	36.0	32.0	36.0	14.0	36.0
10	29.688596254519943	36.0	21.0	36.0	14.0	36.0
11	31.267202763236007	36.0	32.0	36.0	14.0	36.0
12	30.401748610286578	36.0	21.0	36.0	14.0	36.0
13	30.817367370068542	36.0	32.0	36.0	14.0	36.0
14	30.300178099195854	36.0	27.0	36.0	14.0	36.0
15	30.081925630093366	36.0	21.0	36.0	14.0	36.0
16	30.241567272923525	36.0	21.0	36.0	14.0	36.0
17	30.142857142857142	36.0	21.0	36.0	14.0	36.0
18	30.116897835824922	36.0	21.0	36.0	14.0	36.0
19	30.289600086351125	36.0	21.0	36.0	14.0	36.0
20	30.140590425818985	36.0	21.0	36.0	14.0	36.0
21	30.07016028927627	36.0	21.0	36.0	14.0	36.0
22	29.918829942252685	36.0	21.0	36.0	14.0	36.0
23	29.89913109180204	36.0	21.0	36.0	14.0	36.0
24	29.920556964757946	36.0	21.0	36.0	14.0	36.0
25	29.20556964757947	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	2.0
9	5.0
10	1.0
11	1.0
12	0.0
13	2.0
14	29.0
15	67.0
16	160.0
17	292.0
18	453.0
19	496.0
20	552.0
21	562.0
22	537.0
23	568.0
24	513.0
25	530.0
26	587.0
27	622.0
28	746.0
29	710.0
30	831.0
31	1014.0
32	1290.0
33	1673.0
34	2758.0
35	3526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.233376511226254	20.698402417962004	12.392055267702936	24.67616580310881
2	14.690485185385072	22.343353661827408	40.48788385773652	22.478277295051
3	18.4629499703168	26.7148793782719	29.980031302282907	24.84213934912839
4	11.333585190782017	16.530843542554912	38.32370878083005	33.81186248583302
5	12.76515355966967	37.85826091650024	34.57116640578615	14.805419118043936
6	30.93451384764887	37.8286454677968	17.637531717324407	13.59930896722993
7	26.082496490659757	33.26314652845265	22.15203541734154	18.502321563546055
8	25.43333873319294	34.958691074032075	21.07025217344349	18.537718019331496
9	26.59034452964683	14.726212333945352	19.6349497785938	39.04849335781402
10	14.647583040777748	28.5876316500135	33.36753983256819	23.397245476640563
11	34.75395667909037	23.4537892291903	23.507805325987142	18.28444876573219
12	23.28256642903435	25.777705767984443	31.227046878375457	19.712680924605746
13	28.975799481417457	21.980337078651687	27.004105445116682	22.039757994814174
14	21.914330470480202	21.087884189488467	27.87230594717226	29.12547939285907
15	22.704687837545904	29.531216245409375	25.64268740548715	22.121408511557572
16	22.152616514554193	28.238915591078467	27.34784252308689	22.260625371280447
17	20.59188853485986	27.007614624399203	29.42161257223092	22.97888426851002
18	21.362212379820676	26.02354974613806	31.527492708220805	21.08674516582046
19	23.301285513665334	25.288970508804148	29.415577400885816	21.9941665766447
20	22.94355927626249	26.205779098028625	30.013502565487443	20.837159060221442
21	24.5219833639408	25.380792913470884	28.173274278924058	21.923949443664252
22	22.542134831460675	26.436905790838377	29.164866032843562	21.85609334485739
23	21.92513368983957	26.78118079187598	29.19570031869497	22.09798519958948
24	21.74265341400173	26.77182368193604	29.791486603284355	21.694036300777874
25	22.02776427375358	26.289634311024685	29.881704748014908	21.80089666720683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	12.5
3	23.0
4	23.0
5	23.0
6	54.5
7	86.0
8	86.0
9	86.0
10	101.5
11	117.0
12	117.0
13	117.0
14	120.0
15	123.0
16	123.0
17	123.0
18	187.0
19	251.0
20	251.0
21	251.0
22	421.5
23	592.0
24	592.0
25	592.0
26	840.0
27	1088.0
28	1088.0
29	1088.0
30	1372.0
31	1656.0
32	1656.0
33	1656.0
34	1658.0
35	1660.0
36	1660.0
37	1660.0
38	1734.0
39	1808.0
40	1808.0
41	1808.0
42	1900.5
43	1993.0
44	1993.0
45	1993.0
46	2129.5
47	2266.0
48	2266.0
49	2266.0
50	2145.5
51	2025.0
52	2025.0
53	2025.0
54	1749.0
55	1473.0
56	1473.0
57	1473.0
58	1322.0
59	1171.0
60	1171.0
61	1171.0
62	1021.5
63	872.0
64	872.0
65	872.0
66	732.0
67	592.0
68	592.0
69	592.0
70	462.0
71	332.0
72	332.0
73	332.0
74	258.0
75	184.0
76	184.0
77	184.0
78	149.0
79	114.0
80	114.0
81	114.0
82	82.5
83	51.0
84	51.0
85	51.0
86	34.5
87	18.0
88	18.0
89	18.0
90	13.5
91	9.0
92	9.0
93	9.0
94	6.5
95	4.0
96	4.0
97	4.0
98	11.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005396945328943818
2	0.0
3	0.0
4	0.0
5	0.010793890657887635
6	0.03238167197366291
7	0.037778617302606725
8	0.05396945328943818
9	0.059366398618382
10	0.07555723460521345
11	0.08635112526310108
12	0.07016028927626963
13	0.0917480705920449
14	0.08635112526310108
15	0.07016028927626963
16	0.06476334394732582
17	0.06476334394732582
18	0.08095417993415727
19	0.08095417993415727
20	0.07555723460521345
21	0.08095417993415727
22	0.0917480705920449
23	0.08635112526310108
24	0.0917480705920449
25	0.08635112526310108
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	18529.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.26094230665443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.45499430307633	80.53321819849964
2	3.285225977971895	5.602029251443683
3	0.8671983795417141	2.218144530195909
4	0.46208380807697175	1.575908036051595
5	0.2152171160906444	0.9174807059204491
6	0.17090770983668818	0.8743051432888985
7	0.094948727687049	0.5666792595391008
8	0.0696290669705026	0.474931188947056
9	0.0506393214330928	0.3885800636839549
>10	0.2911760982402836	3.7077014409844025
>50	0.0253196607165464	1.268282152301797
>100	0.0126598303582732	1.8727400291435048
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	203	1.095579901775595	No Hit
TATCAACGCAGAGTACTTTTTTTTT	144	0.7771601273679098	No Hit
GGTATCAACGCAGAGTACTTTTTTT	68	0.3669922823681796	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	60	0.32381671973662907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	54	0.29143504776296614	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	53	0.28603810243402233	No Hit
GTACATGGAAGCAGTGGTATCAACG	39	0.2104808678288089	No Hit
TCCATGTACTCTGCGTTGATACCAC	36	0.19429003184197746	No Hit
GAGTACATGGAAGCAGTGGTATCAA	31	0.16730530519725834	No Hit
GTATCAACGCAGAGTACATGGGCAG	26	0.14032057855253927	No Hit
GTTCTACAGTGTGGTTTTTATCATT	25	0.13492363322359543	No Hit
GATATACACTGTTCTACAAATCCCG	22	0.118732797236764	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	20	0.10793890657887636	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	20	0.10793890657887636	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.005396945328943818	0.0
5	0.0	0.0	0.0	0.005396945328943818	0.0
6	0.0	0.0	0.0	0.005396945328943818	0.0
7	0.0	0.0	0.0	0.005396945328943818	0.0
8	0.0	0.0	0.0	0.005396945328943818	0.0
9	0.0	0.0	0.0	0.005396945328943818	0.0
10	0.0	0.0	0.0	0.005396945328943818	0.0
11	0.0	0.0	0.0	0.005396945328943818	0.0
12	0.0	0.0	0.0	0.005396945328943818	0.0
13	0.0	0.0	0.0	0.005396945328943818	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE