FastQCFastQC Report
Thu 2 Feb 2017
SRR4062603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18529
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.2050839224998651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230.12412974256570782No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11333585190782018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG250.002293603335.24
ATTTAGA300.005601858329.3333321
ACATGGG851.3368926E-418.1176473
GTACATG1004.624754E-415.4000011
TACATGG1056.6996843E-414.6666672
Produced by FastQC (version 0.11.5)