FastQCFastQC Report
Thu 2 Feb 2017
SRR4062602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561304
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25930.16607912360437174No Hit
TATCAACGCAGAGTACTTTTTTTTT17570.11253413813069074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG2100.013.57323713
GCATCGC2300.012.80605514
ATAATAC905.419388E-712.6638893
GAGTAGT751.4868208E-512.659831
TCGCCAG2350.012.12927717
AATCGTA951.0380954E-612.001613
CGGTCCA3100.011.9524410
CATCGCC2500.011.7815715
CGACCAT1950.011.69311510
GTCTTAC658.059872E-411.6859971
CAAATCG907.449431E-611.61265913
GTCCTAA1406.85759E-1011.5294891
AGCCCCG2500.011.4015216
CGCCAGT2500.011.4015218
TTTAAAC1751.8189894E-1211.39753
CGTCTTA1850.011.29880415
TAAGCTG1354.7257345E-911.2593165
GTGTTAG1105.002421E-711.2212131
GGTTCTA2900.011.13941613
ATCGCCA2650.011.11468916
Produced by FastQC (version 0.11.5)