##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062602_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561304
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180276871128235	32.0	32.0	32.0	32.0	32.0
2	30.801662584608763	32.0	32.0	32.0	32.0	32.0
3	30.78631771903486	32.0	32.0	32.0	32.0	32.0
4	30.87785722703586	32.0	32.0	32.0	32.0	32.0
5	30.629602562985813	32.0	32.0	32.0	32.0	32.0
6	34.29575790493075	36.0	36.0	36.0	32.0	36.0
7	34.183766902537876	36.0	36.0	36.0	32.0	36.0
8	34.1888043584081	36.0	36.0	36.0	32.0	36.0
9	34.37092840343713	36.0	36.0	36.0	32.0	36.0
10	33.98939604330739	36.0	36.0	36.0	32.0	36.0
11	34.33596404031502	36.0	36.0	36.0	32.0	36.0
12	34.09477270281764	36.0	36.0	36.0	32.0	36.0
13	34.24184399706912	36.0	36.0	36.0	32.0	36.0
14	34.12882821026527	36.0	36.0	36.0	32.0	36.0
15	34.054004857478105	36.0	36.0	36.0	32.0	36.0
16	34.061955903526794	36.0	36.0	36.0	32.0	36.0
17	33.9831390939881	36.0	36.0	36.0	32.0	36.0
18	33.99660155869709	36.0	36.0	36.0	32.0	36.0
19	33.98538593380918	36.0	36.0	36.0	32.0	36.0
20	33.97670793131895	36.0	36.0	36.0	32.0	36.0
21	33.96365794233538	36.0	36.0	36.0	32.0	36.0
22	33.91619377136035	36.0	36.0	36.0	32.0	36.0
23	33.87909913764392	36.0	36.0	36.0	32.0	36.0
24	33.844615782704714	36.0	36.0	36.0	32.0	36.0
25	33.39958585899991	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	14.0
4	63.0
5	146.0
6	253.0
7	71.0
8	236.0
9	270.0
10	178.0
11	70.0
12	110.0
13	78.0
14	279.0
15	377.0
16	543.0
17	688.0
18	913.0
19	1265.0
20	1985.0
21	2814.0
22	4248.0
23	6597.0
24	9298.0
25	13129.0
26	18795.0
27	24294.0
28	33390.0
29	44712.0
30	58005.0
31	79067.0
32	113972.0
33	164019.0
34	359699.0
35	621725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95363422247246	17.62911605568377	11.0263244368177	26.390925285026068
2	17.192123716728457	19.979452282923106	36.46124500718658	26.36717899316186
3	19.023208861821633	23.430868542044845	28.55816050842485	28.987762087708667
4	12.973601440037925	15.198934064455791	35.689751260353475	36.137713235152816
5	14.864555762506942	36.088029303804	33.60499707512976	15.442417858559299
6	34.25109235922038	35.11549628255051	16.770333195994137	13.863078162234968
7	30.26017258529282	30.565247597854732	20.7606750617841	18.413904755068348
8	28.114943471894925	32.532892459414896	19.698358646402536	19.653805422287636
9	27.366648245761375	14.416837305795148	18.823622182531082	39.392892265912394
10	16.12371416955969	26.33732136879945	31.610556730435054	25.92840773120581
11	37.16116000577117	21.222334439474825	22.44434825822793	19.172157296526073
12	24.65574914322067	23.859316320876687	28.41448367501898	23.07045086088366
13	29.20403238786436	19.385779683264794	25.279629721066026	26.130558207804818
14	23.382478739353648	19.880483810359266	24.847478668816137	31.88955878147095
15	25.166437653926376	27.039298073279376	22.213231272517895	25.581033000276353
16	25.844110128167397	25.77088627155279	23.592059764003743	24.792943836276073
17	24.139565127058106	25.76418261893341	25.134214502091773	24.962037751916707
18	24.945841707720938	24.777627142122387	26.06780656671699	24.208724583439682
19	25.49169373002314	25.161995351772333	25.30904813942631	24.037262778778224
20	25.42374946454176	24.626590022393057	25.019751034364234	24.929909478700946
21	26.525483023395857	24.25721144133912	24.43402234217687	24.78328319308815
22	25.676978466642247	24.60906606252762	24.711053012046648	25.00290245878348
23	24.534617409814384	24.363888940553526	25.331884728139602	25.76960892149248
24	24.649681732912185	25.256649938270613	25.0330131956581	25.0606551331591
25	24.66455973023689	24.729589160222513	25.27990231476553	25.325948794775073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	293.0
2	592.5
3	892.0
4	892.0
5	892.0
6	2159.0
7	3426.0
8	3426.0
9	3426.0
10	3655.5
11	3885.0
12	3885.0
13	3885.0
14	4602.5
15	5320.0
16	5320.0
17	5320.0
18	8453.0
19	11586.0
20	11586.0
21	11586.0
22	18093.0
23	24600.0
24	24600.0
25	24600.0
26	36151.5
27	47703.0
28	47703.0
29	47703.0
30	57629.5
31	67556.0
32	67556.0
33	67556.0
34	83274.0
35	98992.0
36	98992.0
37	98992.0
38	111090.0
39	123188.0
40	123188.0
41	123188.0
42	139399.5
43	155611.0
44	155611.0
45	155611.0
46	177364.0
47	199117.0
48	199117.0
49	199117.0
50	202103.0
51	205089.0
52	205089.0
53	205089.0
54	192089.5
55	179090.0
56	179090.0
57	179090.0
58	164540.0
59	149990.0
60	149990.0
61	149990.0
62	133073.0
63	116156.0
64	116156.0
65	116156.0
66	96883.0
67	77610.0
68	77610.0
69	77610.0
70	59378.0
71	41146.0
72	41146.0
73	41146.0
74	32563.0
75	23980.0
76	23980.0
77	23980.0
78	19370.0
79	14760.0
80	14760.0
81	14760.0
82	10494.5
83	6229.0
84	6229.0
85	6229.0
86	4664.0
87	3099.0
88	3099.0
89	3099.0
90	2097.5
91	1096.0
92	1096.0
93	1096.0
94	656.5
95	217.0
96	217.0
97	217.0
98	445.0
99	673.0
100	673.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008390422364894986
2	0.003842941541173276
3	0.005764412311759914
4	0.014987472010575775
5	0.03529101315310792
6	0.044257876749178894
7	0.06609859450818034
8	0.08787526324149557
9	0.09492065606697991
10	0.11567254038931561
11	0.11714566798009869
12	0.12656087475597322
13	0.11727376603147112
14	0.11849069751950934
15	0.10958788294912458
16	0.1090754907436348
17	0.11913118777637155
18	0.1272013650128354
19	0.1280980513724425
20	0.12118075659833064
21	0.12880259065499094
22	0.1460958275902707
23	0.13469510101812332
24	0.133157924401654
25	0.12873854162930473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1561304.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.99084301152506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22127003685385	46.03638222841694
2	10.956358083472779	12.269114508595559
3	2.8864308354982873	4.848410873220291
4	1.187054502740139	2.658567292361882
5	0.6621105113874252	1.8536062849686952
6	0.4228629922971741	1.4205873250256886
7	0.28439506375741785	1.1146463557665969
8	0.20956015930622746	0.9386759984948132
9	0.15031884065757012	0.7574830748039213
>10	0.8276695018985503	8.532318475287829
>50	0.08654235538802924	3.431419516362031
>100	0.09418473831106307	11.105788061304615
>500	0.008718579191775051	3.190450671513518
>1k	0.0025237992397243573	1.8425493338776504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2593	0.16607912360437174	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1757	0.11253413813069074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.404902568622127E-5
7	0.0	0.0	0.0	6.404902568622127E-5	6.404902568622127E-5
8	0.0	0.0	0.0	6.404902568622127E-5	1.2809805137244253E-4
9	0.0	0.0	0.0	6.404902568622127E-5	1.2809805137244253E-4
10	0.0	0.0	0.0	6.404902568622127E-5	1.2809805137244253E-4
11	0.0	0.0	0.0	6.404902568622127E-5	1.2809805137244253E-4
12	0.0	0.0	0.0	6.404902568622127E-5	1.9214707705866377E-4
13	0.0	0.0	0.0	6.404902568622127E-5	1.9214707705866377E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	210	0.0	13.573237	13
GCATCGC	230	0.0	12.806055	14
ATAATAC	90	5.419388E-7	12.663889	3
GAGTAGT	75	1.4868208E-5	12.65983	1
TCGCCAG	235	0.0	12.129277	17
AATCGTA	95	1.0380954E-6	12.0016	13
CGGTCCA	310	0.0	11.95244	10
CATCGCC	250	0.0	11.78157	15
CGACCAT	195	0.0	11.693115	10
GTCTTAC	65	8.059872E-4	11.685997	1
CAAATCG	90	7.449431E-6	11.612659	13
GTCCTAA	140	6.85759E-10	11.529489	1
AGCCCCG	250	0.0	11.40152	16
CGCCAGT	250	0.0	11.40152	18
TTTAAAC	175	1.8189894E-12	11.3975	3
CGTCTTA	185	0.0	11.298804	15
TAAGCTG	135	4.7257345E-9	11.259316	5
GTGTTAG	110	5.002421E-7	11.221213	1
GGTTCTA	290	0.0	11.139416	13
ATCGCCA	265	0.0	11.114689	16
>>END_MODULE