##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561304
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246827011267506	32.0	32.0	32.0	32.0	32.0
2	31.366150986611192	32.0	32.0	32.0	32.0	32.0
3	31.460971085707843	32.0	32.0	32.0	32.0	32.0
4	31.570191967739788	32.0	32.0	32.0	32.0	32.0
5	31.490101223080195	32.0	32.0	32.0	32.0	32.0
6	35.08201413690095	36.0	36.0	36.0	36.0	36.0
7	35.09914661078176	36.0	36.0	36.0	36.0	36.0
8	35.03511551882273	36.0	36.0	36.0	36.0	36.0
9	35.17896834953347	36.0	36.0	36.0	36.0	36.0
10	35.029901287641614	36.0	36.0	36.0	36.0	36.0
11	35.187404887196855	36.0	36.0	36.0	36.0	36.0
12	35.08590255325036	36.0	36.0	36.0	36.0	36.0
13	35.13853612108853	36.0	36.0	36.0	36.0	36.0
14	35.09280960018036	36.0	36.0	36.0	36.0	36.0
15	35.048435154204434	36.0	36.0	36.0	36.0	36.0
16	35.07393243083986	36.0	36.0	36.0	36.0	36.0
17	35.041332117255834	36.0	36.0	36.0	36.0	36.0
18	35.05193927639973	36.0	36.0	36.0	36.0	36.0
19	35.03685380937985	36.0	36.0	36.0	36.0	36.0
20	35.0383794571717	36.0	36.0	36.0	36.0	36.0
21	35.03145127406322	36.0	36.0	36.0	36.0	36.0
22	35.01160568345434	36.0	36.0	36.0	36.0	36.0
23	34.97345295983357	36.0	36.0	36.0	36.0	36.0
24	34.94618985155998	36.0	36.0	36.0	32.0	36.0
25	34.92695464816589	36.0	36.0	36.0	32.0	36.0
26	34.879705681917166	36.0	36.0	36.0	32.0	36.0
27	34.86827805475423	36.0	36.0	36.0	32.0	36.0
28	34.85592812162142	36.0	36.0	36.0	32.0	36.0
29	34.836870334028475	36.0	36.0	36.0	32.0	36.0
30	34.81492969978941	36.0	36.0	36.0	32.0	36.0
31	34.81782919918222	36.0	36.0	36.0	32.0	36.0
32	34.78635358648924	36.0	36.0	36.0	32.0	36.0
33	34.766335063511015	36.0	36.0	36.0	32.0	36.0
34	34.74771665223429	36.0	36.0	36.0	32.0	36.0
35	34.725485235418596	36.0	36.0	36.0	32.0	36.0
36	34.69640697775706	36.0	36.0	36.0	32.0	36.0
37	34.69610979027787	36.0	36.0	36.0	32.0	36.0
38	34.66001111891086	36.0	36.0	36.0	32.0	36.0
39	34.65912916382716	36.0	36.0	36.0	32.0	36.0
40	34.64972612636617	36.0	36.0	36.0	32.0	36.0
41	34.61788735569755	36.0	36.0	36.0	32.0	36.0
42	34.59581349948505	36.0	36.0	36.0	32.0	36.0
43	34.57654627157812	36.0	36.0	36.0	32.0	36.0
44	34.55644384437624	36.0	36.0	36.0	32.0	36.0
45	34.52004350209825	36.0	36.0	36.0	32.0	36.0
46	34.5251725480752	36.0	36.0	36.0	32.0	36.0
47	34.49817780521923	36.0	36.0	36.0	32.0	36.0
48	34.45323588487572	36.0	36.0	36.0	32.0	36.0
49	34.447075649585216	36.0	36.0	36.0	32.0	36.0
50	33.92136637067477	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	31.0
22	116.0
23	381.0
24	1024.0
25	2475.0
26	5015.0
27	9244.0
28	15399.0
29	24385.0
30	35182.0
31	51008.0
32	75452.0
33	125093.0
34	276213.0
35	940275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28945067348598	18.418417777185585	11.553133549896	26.738997999432435
2	16.633861735059558	20.49794747666772	36.11601993019691	26.752170858075807
3	18.4183355836064	23.826869028983687	28.979972996723223	28.774822390686687
4	12.677752286232902	15.713808090939485	35.91476857135903	35.693671051468584
5	14.62264876026706	36.340136193848224	33.47413444146687	15.563080604417845
6	34.50855282499102	35.40210478851878	16.452859385498083	13.636483000992126
7	30.254960446448187	30.64969535022395	20.678459822929725	18.416884380398145
8	28.1056091574735	32.773245953382556	19.484994594262233	19.636150294881713
9	27.48497409857401	14.137413341668248	18.689505695239365	39.688106864518375
10	15.999894959597874	26.509827682501292	31.590196399932363	25.90008095796847
11	37.456318564482	21.099094090580696	22.310517362409882	19.13406998252743
12	24.615129404651494	23.877348677771913	28.337146385329188	23.1703755322474
13	29.406572967212025	19.25704411184497	25.259334505003512	26.077048415939498
14	23.416003545754062	19.722552430532428	24.933132817183584	31.928311206529926
15	25.37423845708459	27.01920958378381	22.18568581134744	25.42086614778416
16	26.00518540911956	25.751551267402125	23.471213805895587	24.77204951758274
17	24.299367708018426	25.772303151724458	25.017165138883907	24.911164001373212
18	24.994491783790984	24.64574483892951	26.09850483954438	24.261258537735124
19	25.651570738305928	25.062768045172497	25.29641889087583	23.989242325645744
20	25.550693522850132	24.364441518115626	25.065009761071515	25.019855197962727
21	26.61493213365238	24.185168295219896	24.41439975815088	24.785499812976845
22	25.75276819889016	24.550439888708414	24.742458867715705	24.954333044685725
23	24.576315695085647	24.271890675999035	25.36559183861695	25.786201790298367
24	24.81528260992094	25.035483160230164	25.030551385252327	25.118682844596567
25	24.90251738290557	24.41881914092323	25.369562878209496	25.309100597961702
26	24.568501713951925	25.044770268954668	25.67302716191081	24.71370085518259
27	25.315505500530328	24.90725701080635	24.804650471657023	24.972587017006298
28	24.671748743358116	24.581247470063484	25.613653715099687	25.13335007147871
29	24.67898628326066	24.921988286714182	25.427335099378467	24.97169033064669
30	24.812336354739372	24.836482837423077	25.669952808677877	24.681227999159677
31	25.068660555535626	24.67776935177262	24.807724824889966	25.445845267801786
32	24.655031947654013	24.814514021612702	24.97649400757316	25.553960023160126
33	24.55825386984213	24.47627111696377	25.41010591146887	25.555369101725223
34	25.11112505956559	24.44559163366007	25.756418993354274	24.686864313420067
35	25.72785312789822	24.496894903234733	25.252609357306454	24.522642611560595
36	24.673478067051644	24.685455234854967	25.241464826837056	25.399601871256333
37	25.608273596942045	24.58477016647623	24.797989372985658	25.008966863596072
38	24.693269215988686	24.44456684924909	25.61346156802263	25.248702366739593
39	25.00595655938882	24.543458544908614	24.829565542648965	25.621019353053605
40	25.336897875109525	24.50201882528963	25.633637011113784	24.52744628848706
41	24.402806884501675	24.7094736194873	25.816689126525006	25.07103036948602
42	25.354895651327354	24.911676393578702	25.27496246727095	24.458465487823
43	24.510217100577467	24.12182380881622	25.736948089545663	25.63101100106065
44	24.393840020905603	24.46422990013476	25.60308562586146	25.538844453098182
45	24.35131146784995	24.723308209035526	25.693330703053345	25.232049620061183
46	24.31342558966811	24.362487206190732	25.781110893344145	25.542976310797016
47	24.452460540971227	24.21439016001378	26.039788561220977	25.293360737794007
48	24.82758002285269	25.07307993830798	25.26785302541978	24.83148701341955
49	23.944856350845193	24.915839580248306	25.726059755179005	25.4132443137275
50	23.68010801241528	25.27768490657157	25.642124329565963	25.40008275144719
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	43.5
2	50.0
3	166.0
4	282.0
5	305.0
6	328.0
7	285.5
8	243.0
9	360.5
10	478.0
11	678.0
12	878.0
13	1467.5
14	2057.0
15	2677.5
16	3298.0
17	3809.0
18	4320.0
19	4586.5
20	4853.0
21	5765.0
22	6677.0
23	7156.0
24	7635.0
25	9558.5
26	11482.0
27	14480.0
28	17478.0
29	19601.5
30	21725.0
31	23292.0
32	24859.0
33	28710.5
34	32562.0
35	37951.0
36	43340.0
37	52167.5
38	60995.0
39	65077.5
40	69160.0
41	75691.0
42	82222.0
43	83326.0
44	84430.0
45	91799.0
46	99168.0
47	105108.0
48	111048.0
49	113283.5
50	115519.0
51	112389.0
52	109259.0
53	107416.5
54	105574.0
55	106567.0
56	107560.0
57	105194.5
58	102829.0
59	93953.0
60	85077.0
61	75639.5
62	66202.0
63	58464.0
64	50726.0
65	43431.0
66	36136.0
67	32127.5
68	28119.0
69	25494.5
70	22870.0
71	18252.5
72	13635.0
73	12165.5
74	10696.0
75	8027.0
76	5358.0
77	4836.0
78	4314.0
79	3735.0
80	3156.0
81	2528.5
82	1901.0
83	1621.0
84	1341.0
85	1099.5
86	858.0
87	617.0
88	376.0
89	252.0
90	128.0
91	83.5
92	39.0
93	26.0
94	13.0
95	9.5
96	6.0
97	5.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015820109344496652
2	0.0035226964127421695
3	7.685883082346552E-4
4	1.280980513724425E-4
5	0.0
6	4.4834317980354887E-4
7	1.921470770586638E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.280980513724425E-4
47	6.404902568622125E-5
48	0.0
49	0.0
50	1.280980513724425E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1561304.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.52003041876716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09965700935274	49.460941129857936
2	10.655032011222042	12.683756588417477
3	2.7065782541826153	4.83286860059169
4	1.0869666962781328	2.5878516330664527
5	0.6225735303197966	1.8527797731276778
6	0.35019287695171814	1.250609441316111
7	0.2521183713012422	1.0504265190285982
8	0.19490575557254985	0.9280637200376773
9	0.14782850893534197	0.7918881613733293
>10	0.7220796032745226	7.871280361394704
>50	0.07639210308522236	3.1765096502655648
>100	0.07714028972740086	9.696251092456254
>500	0.007130497804830385	2.773824182000136
>1k	0.0014044919918605305	1.0429491470664922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.404902568622127E-5
2	0.0	0.0	0.0	0.0	6.404902568622127E-5
3	0.0	0.0	0.0	0.0	6.404902568622127E-5
4	0.0	0.0	0.0	6.404902568622127E-5	6.404902568622127E-5
5	0.0	0.0	0.0	6.404902568622127E-5	6.404902568622127E-5
6	0.0	0.0	0.0	6.404902568622127E-5	1.2809805137244253E-4
7	0.0	0.0	0.0	1.2809805137244253E-4	1.2809805137244253E-4
8	0.0	0.0	0.0	1.2809805137244253E-4	1.2809805137244253E-4
9	0.0	0.0	0.0	1.9214707705866377E-4	1.9214707705866377E-4
10	0.0	0.0	0.0	1.9214707705866377E-4	1.9214707705866377E-4
11	0.0	0.0	0.0	1.9214707705866377E-4	1.9214707705866377E-4
12	0.0	0.0	0.0	1.9214707705866377E-4	3.202451284311063E-4
13	0.0	0.0	0.0	1.9214707705866377E-4	4.483431798035488E-4
14	0.0	0.0	0.0	2.5619610274488507E-4	4.483431798035488E-4
15	0.0	0.0	0.0	2.5619610274488507E-4	6.404902568622126E-4
16	0.0	0.0	0.0	2.5619610274488507E-4	8.326373339208764E-4
17	0.0	0.0	0.0	3.202451284311063E-4	8.326373339208764E-4
18	0.0	0.0	0.0	4.483431798035488E-4	9.60735385293319E-4
19	0.0	0.0	0.0	5.123922054897701E-4	9.60735385293319E-4
20	0.0	0.0	0.0	7.685883082346551E-4	0.0010888334366657615
21	0.0	0.0	0.0	8.966863596070976E-4	0.0010888334366657615
22	0.0	0.0	0.0	0.0013450295394106465	0.0010888334366657615
23	0.0	0.0	0.0	0.0017933727192141953	0.0011528824623519827
24	0.0	0.0	0.0	0.0030103042072523994	0.001216931488038204
25	0.0	0.0	0.0	0.0037788925154870544	0.001216931488038204
26	0.0	0.0	0.0	0.004355333746663046	0.001216931488038204
27	0.0	0.0	0.0	0.006276804517249684	0.001216931488038204
28	0.0	0.0	0.0	0.013450295394106465	0.001216931488038204
29	0.0	0.0	0.0	0.028629914481740903	0.001216931488038204
30	0.0	0.0	0.0	0.0641771237375937	0.001216931488038204
31	0.0	0.0	0.0	0.1367446698400824	0.001216931488038204
32	0.0	0.0	0.0	0.22289060938805	0.0012809805137244251
33	0.0	0.0	0.0	0.3174910203265988	0.0012809805137244251
34	0.0	0.0	0.0	0.41945706921906306	0.0012809805137244251
35	0.0	0.0	0.0	0.5400613845862177	0.0012809805137244251
36	0.0	0.0	0.0	0.7089586653207831	0.0012809805137244251
37	0.0	0.0	0.0	0.9588779635484185	0.0012809805137244251
38	0.0	0.0	0.0	1.268170708587181	0.0013450295394106465
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTA	395	0.0	19.4936	42
TAACGGC	280	0.0	18.857075	36
GTATCAA	2580	0.0	18.420307	1
CGGATAC	60	0.0074132015	18.333267	29
TTAACGG	300	0.0	17.599936	35
CGTCGAA	75	0.0012911307	17.599936	22
ACCGTCG	370	0.0	17.24318	8
GTATTAG	540	0.0	16.705248	1
ATACCGT	370	0.0	16.648588	6
CGGCGTT	465	0.0	16.559078	41
GGACCGT	120	6.4091637E-6	16.49994	6
TAGGACG	300	0.0	16.133274	4
CGTTATT	505	0.0	16.118753	44
CGTCGTA	360	0.0	15.888831	10
GCGTTAT	485	0.0	15.87623	43
CTATAGA	295	0.0	15.662465	1
AGCGAAA	520	0.0	15.65379	17
TAGCGTA	325	0.0	15.569174	7
GCGAAAG	540	0.0	15.481425	18
CGCAATA	455	0.0	15.472471	36
>>END_MODULE