FastQCFastQC Report
Thu 2 Feb 2017
SRR4062601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794106
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32630.18187331183330305No Hit
GCGCAAGACGGACCAGAGCGAAAGC23860.1329910272860132No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23450.13070576654891072No Hit
TATCAACGCAGAGTACTTTTTTTTT23200.12931231487994577No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21720.12106308099967339No Hit
GATTAAGAGGGACGGCCGGGGGCAT20170.11242368065209078No Hit
GAATAACGCCGCCGCATCGCCAGTC19930.11108596704988444No Hit
GAATAGGACCGCGGTTCTATTTTGT19590.10919087278009215No Hit
GAACTACGACGGTATCTGATCGTCT19430.1082990637119546No Hit
GTATCTGATCGTCTTCGAACCTCCG18730.10439739903885278No Hit
GTACATGGAAGCAGTGGTATCAACG18400.10255804283581905No Hit
GGGTAGGCACACGCTGAGCCAGTCA18390.10250230476906046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG4850.013.3182964
AAGACGG4800.012.6661465
AAGACCG853.9460774E-612.29361255
TCAATAC550.003074200112.0880513
ACTCTAA3400.012.015552510
CGTCTTA1201.0009899E-811.87550615
TAGATTG802.8681405E-511.8745135
TCGGCGT2400.011.47933613
CGGTCCA4100.011.3544610
CGCAAGA5300.011.2882092
GCAAGAC6300.011.1560943
GATAACG1803.6379788E-1211.08349611
GCGCAAG5350.011.0049011
CGGACCA5500.010.8825859
GCACCGT1053.4636505E-610.8579086
TAGGAGT1408.363713E-910.8560914
GTACGAG700.001496627910.852761
GTGTTAG1053.4833865E-610.8527591
CGCCGGT4300.010.8263447
TTTGAAC4750.010.7974473
Produced by FastQC (version 0.11.5)