##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062601_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794106
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183454043406577	32.0	32.0	32.0	32.0	32.0
2	30.8354684728773	32.0	32.0	32.0	32.0	32.0
3	30.81539440813419	32.0	32.0	32.0	32.0	32.0
4	30.893469505146296	32.0	32.0	32.0	32.0	32.0
5	30.685491827127272	32.0	32.0	32.0	32.0	32.0
6	34.33642438072221	36.0	36.0	36.0	32.0	36.0
7	34.218616960201906	36.0	36.0	36.0	32.0	36.0
8	34.222851938514225	36.0	36.0	36.0	32.0	36.0
9	34.38554633895656	36.0	36.0	36.0	32.0	36.0
10	34.04938281238678	36.0	36.0	36.0	32.0	36.0
11	34.352424550165935	36.0	36.0	36.0	32.0	36.0
12	34.13589665270614	36.0	36.0	36.0	32.0	36.0
13	34.26158878015011	36.0	36.0	36.0	32.0	36.0
14	34.163139747595736	36.0	36.0	36.0	32.0	36.0
15	34.10355296732746	36.0	36.0	36.0	32.0	36.0
16	34.09909503674811	36.0	36.0	36.0	32.0	36.0
17	34.01809703551518	36.0	36.0	36.0	32.0	36.0
18	34.03388540030522	36.0	36.0	36.0	32.0	36.0
19	34.02482796445695	36.0	36.0	36.0	32.0	36.0
20	34.016851847103794	36.0	36.0	36.0	32.0	36.0
21	33.99461848965446	36.0	36.0	36.0	32.0	36.0
22	33.95383773311053	36.0	36.0	36.0	32.0	36.0
23	33.917429627903815	36.0	36.0	36.0	32.0	36.0
24	33.88881816347529	36.0	36.0	36.0	32.0	36.0
25	33.433322222878694	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	63.0
5	153.0
6	305.0
7	67.0
8	275.0
9	304.0
10	200.0
11	55.0
12	155.0
13	96.0
14	346.0
15	435.0
16	654.0
17	785.0
18	1075.0
19	1550.0
20	2389.0
21	3545.0
22	5266.0
23	7728.0
24	11081.0
25	15405.0
26	21228.0
27	27558.0
28	37046.0
29	49369.0
30	63801.0
31	87297.0
32	124894.0
33	182268.0
34	409078.0
35	739626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67311739008528	17.139856596499698	10.990663666226485	26.196362347188533
2	17.5065187849141	19.573299681279074	35.81062024050665	27.10956129330017
3	19.306245805546823	22.74625481316986	28.058578534655936	29.888920846627382
4	13.224677874490068	14.729584038049664	34.58257194238589	37.46316614507438
5	15.387163034678405	35.71659563269302	32.85889998817959	16.03734134444898
6	35.55280087748206	34.019828105857705	16.235146904081997	14.192224112578227
7	31.18768283466587	29.866169904821398	20.13977004314131	18.80637721737142
8	28.912993029088607	31.877917562947957	19.194496014709426	20.014593393254014
9	27.33848350259117	14.249291326370358	18.48113709131666	39.93108807972181
10	16.62269467927792	26.05384894394688	30.498590999135068	26.824865377640133
11	38.005962993282004	20.886993855650474	21.47654115140568	19.63050199966184
12	25.019281618785378	23.33405139458284	27.645990595257892	24.00067639137389
13	29.593228359286677	18.636966801371827	25.02206954893302	26.74773529040848
14	23.8376249093889	19.297667018787397	24.16748000417411	32.69722806764959
15	25.601233826670565	26.6062289498412	21.751384372272824	26.041152851215415
16	26.57949772117544	25.214992924707282	22.953131207620782	25.2523781464965
17	24.658131272011154	25.50914096012866	24.511366978257122	25.32136078960306
18	25.668122114775034	24.07784415097372	25.498859303706485	24.755174430544766
19	25.93946652320372	24.7579062743461	24.661024137938732	24.64160306451145
20	25.94323302984105	23.844605106616637	24.430700859584782	25.781461003957528
21	27.130889590701106	23.841028557333154	24.001866207853364	25.026215644112376
22	26.322091031332313	23.802578456375727	24.234163179437544	25.641167332854415
23	24.72591473845599	23.66015939167946	24.988460596075097	26.62546527378945
24	25.070319877308556	24.612570961685712	24.62992769330711	25.687181467698622
25	25.337006948579617	24.043949445190712	24.538123989537226	26.080919616692437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	227.0
1	227.0
2	553.0
3	879.0
4	879.0
5	879.0
6	2115.0
7	3351.0
8	3351.0
9	3351.0
10	3604.0
11	3857.0
12	3857.0
13	3857.0
14	4242.5
15	4628.0
16	4628.0
17	4628.0
18	7341.5
19	10055.0
20	10055.0
21	10055.0
22	16007.0
23	21959.0
24	21959.0
25	21959.0
26	32548.0
27	43137.0
28	43137.0
29	43137.0
30	53208.5
31	63280.0
32	63280.0
33	63280.0
34	81437.0
35	99594.0
36	99594.0
37	99594.0
38	115235.5
39	130877.0
40	130877.0
41	130877.0
42	152704.5
43	174532.0
44	174532.0
45	174532.0
46	202530.5
47	230529.0
48	230529.0
49	230529.0
50	236469.0
51	242409.0
52	242409.0
53	242409.0
54	229046.0
55	215683.0
56	215683.0
57	215683.0
58	202186.0
59	188689.0
60	188689.0
61	188689.0
62	168431.5
63	148174.0
64	148174.0
65	148174.0
66	123296.0
67	98418.0
68	98418.0
69	98418.0
70	74914.5
71	51411.0
72	51411.0
73	51411.0
74	40383.5
75	29356.0
76	29356.0
77	29356.0
78	24257.5
79	19159.0
80	19159.0
81	19159.0
82	13558.5
83	7958.0
84	7958.0
85	7958.0
86	5870.5
87	3783.0
88	3783.0
89	3783.0
90	2510.0
91	1237.0
92	1237.0
93	1237.0
94	722.5
95	208.0
96	208.0
97	208.0
98	462.0
99	716.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008193495813513807
2	0.0033442840055158394
3	0.0039016646731018126
4	0.013377136022063358
5	0.0333313639216412
6	0.04420028693956767
7	0.06471189550673148
8	0.08438743307251634
9	0.09324978568713331
10	0.11459746525567609
11	0.11443025105540029
12	0.12507622180629238
13	0.11392860845457291
14	0.11744010666036454
15	0.10913513471333355
16	0.10891218244629916
17	0.11799748732795053
18	0.12362703207056885
19	0.1247417934057408
20	0.11582370272436524
21	0.12468605533898221
22	0.14319109350283651
23	0.1348303834890469
24	0.12786312514422224
25	0.12424015080491342
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1794106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.46201673827879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70770836987973	41.53381439038874
2	11.275399235268367	11.605095683523134
3	3.226934784017278	4.981937156052938
4	1.382254096263545	2.84534333753876
5	0.7944499319353524	2.0441997847490776
6	0.5307174837902265	1.6387075220465903
7	0.3515364586535543	1.2663542583541125
8	0.25499367798207445	1.0497991139575031
9	0.18981506093916312	0.879143925890576
>10	1.0924086777098896	10.082556745250475
>50	0.08613564137874702	3.1161073670489254
>100	0.08881702818496882	10.001301240487525
>500	0.013387486367966026	4.711445602017495
>1k	0.005442067629254482	4.244193872694188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3263	0.18187331183330305	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2386	0.1329910272860132	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2345	0.13070576654891072	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2320	0.12931231487994577	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2172	0.12106308099967339	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2017	0.11242368065209078	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1993	0.11108596704988444	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1959	0.10919087278009215	No Hit
GAACTACGACGGTATCTGATCGTCT	1943	0.1082990637119546	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1873	0.10439739903885278	No Hit
GTACATGGAAGCAGTGGTATCAACG	1840	0.10255804283581905	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1839	0.10250230476906046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5738066758597315E-5
2	0.0	0.0	0.0	0.0	5.5738066758597315E-5
3	0.0	0.0	0.0	0.0	5.5738066758597315E-5
4	0.0	0.0	0.0	5.5738066758597315E-5	5.5738066758597315E-5
5	0.0	0.0	0.0	1.6721420027579196E-4	1.1147613351719463E-4
6	5.5738066758597315E-5	0.0	0.0	1.6721420027579196E-4	1.1147613351719463E-4
7	5.5738066758597315E-5	5.5738066758597315E-5	0.0	2.2295226703438926E-4	1.1147613351719463E-4
8	5.5738066758597315E-5	5.5738066758597315E-5	0.0	2.2295226703438926E-4	1.1147613351719463E-4
9	5.5738066758597315E-5	5.5738066758597315E-5	0.0	2.786903337929866E-4	1.1147613351719463E-4
10	5.5738066758597315E-5	1.1147613351719463E-4	0.0	2.786903337929866E-4	1.1147613351719463E-4
11	5.5738066758597315E-5	1.1147613351719463E-4	0.0	3.3442840055158393E-4	1.6721420027579196E-4
12	5.5738066758597315E-5	1.1147613351719463E-4	0.0	3.3442840055158393E-4	3.901664673101812E-4
13	5.5738066758597315E-5	1.1147613351719463E-4	0.0	3.3442840055158393E-4	4.459045340687785E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	485	0.0	13.318296	4
AAGACGG	480	0.0	12.666146	5
AAGACCG	85	3.9460774E-6	12.2936125	5
TCAATAC	55	0.0030742001	12.088051	3
ACTCTAA	340	0.0	12.0155525	10
CGTCTTA	120	1.0009899E-8	11.875506	15
TAGATTG	80	2.8681405E-5	11.874513	5
TCGGCGT	240	0.0	11.479336	13
CGGTCCA	410	0.0	11.35446	10
CGCAAGA	530	0.0	11.288209	2
GCAAGAC	630	0.0	11.156094	3
GATAACG	180	3.6379788E-12	11.083496	11
GCGCAAG	535	0.0	11.004901	1
CGGACCA	550	0.0	10.882585	9
GCACCGT	105	3.4636505E-6	10.857908	6
TAGGAGT	140	8.363713E-9	10.856091	4
GTACGAG	70	0.0014966279	10.85276	1
GTGTTAG	105	3.4833865E-6	10.852759	1
CGCCGGT	430	0.0	10.826344	7
TTTGAAC	475	0.0	10.797447	3
>>END_MODULE