FastQCFastQC Report
Thu 2 Feb 2017
SRR4062601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794106
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24060.13410578862118513No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22850.12736148254339488No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20370.11353844198726275No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20350.11342696585374555No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20110.1120892522515392No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19190.10696135010974826No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18320.1021121383017503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG602.8737623E-421.9997355
GTTATAT2150.020.4739991
TAGACGG550.00448475619.999765
CGCAATA7350.018.557636
ATACGAA7400.018.13491640
GTATAGA2400.017.4242271
ATACCGT5250.017.1807466
GTATCAA34000.017.1545031
CAATACG8100.016.83930438
GTATTAC1702.4556357E-916.8308321
CTAGCGG7850.016.81508429
TCTAGCG8000.016.77479728
CGAATGC8000.016.77479743
TACGAAT8250.016.53313441
GCCGGTC8650.016.5315918
GTATTAT1206.383618E-616.5071621
TTACGCT800.001989015416.4998024
CGCCGGT8150.016.4660597
TACCGTC5350.016.44847
ACCGTCG4850.016.32978
Produced by FastQC (version 0.11.5)