##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062601_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794106
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275832085729604	32.0	32.0	32.0	32.0	32.0
2	31.410735486086107	32.0	32.0	32.0	32.0	32.0
3	31.486321321036773	32.0	32.0	32.0	32.0	32.0
4	31.58485730497529	32.0	32.0	32.0	32.0	32.0
5	31.51694548705595	32.0	32.0	32.0	32.0	32.0
6	35.112463812060156	36.0	36.0	36.0	36.0	36.0
7	35.121684003063365	36.0	36.0	36.0	36.0	36.0
8	35.06048249100109	36.0	36.0	36.0	36.0	36.0
9	35.19041405580272	36.0	36.0	36.0	36.0	36.0
10	35.06023055493934	36.0	36.0	36.0	36.0	36.0
11	35.20152042298504	36.0	36.0	36.0	36.0	36.0
12	35.107391647985125	36.0	36.0	36.0	36.0	36.0
13	35.15201610161272	36.0	36.0	36.0	36.0	36.0
14	35.10616708265844	36.0	36.0	36.0	36.0	36.0
15	35.06964638655687	36.0	36.0	36.0	36.0	36.0
16	35.08332618028143	36.0	36.0	36.0	36.0	36.0
17	35.05061852532682	36.0	36.0	36.0	36.0	36.0
18	35.05869106953547	36.0	36.0	36.0	36.0	36.0
19	35.04854451186273	36.0	36.0	36.0	36.0	36.0
20	35.04147190857174	36.0	36.0	36.0	36.0	36.0
21	35.03941015748234	36.0	36.0	36.0	36.0	36.0
22	35.022959624459205	36.0	36.0	36.0	36.0	36.0
23	34.98524390420633	36.0	36.0	36.0	36.0	36.0
24	34.95784028368446	36.0	36.0	36.0	32.0	36.0
25	34.929482427459696	36.0	36.0	36.0	32.0	36.0
26	34.887512777951805	36.0	36.0	36.0	32.0	36.0
27	34.87631890200468	36.0	36.0	36.0	32.0	36.0
28	34.86509882916617	36.0	36.0	36.0	32.0	36.0
29	34.841094115955244	36.0	36.0	36.0	32.0	36.0
30	34.820293226821605	36.0	36.0	36.0	32.0	36.0
31	34.8277303570692	36.0	36.0	36.0	32.0	36.0
32	34.788803448625664	36.0	36.0	36.0	32.0	36.0
33	34.76856997301163	36.0	36.0	36.0	32.0	36.0
34	34.749946770146245	36.0	36.0	36.0	32.0	36.0
35	34.72869496005253	36.0	36.0	36.0	32.0	36.0
36	34.70422483398417	36.0	36.0	36.0	32.0	36.0
37	34.69883050388327	36.0	36.0	36.0	32.0	36.0
38	34.65933172287479	36.0	36.0	36.0	32.0	36.0
39	34.656827411535325	36.0	36.0	36.0	32.0	36.0
40	34.64007979461637	36.0	36.0	36.0	32.0	36.0
41	34.61471897424121	36.0	36.0	36.0	32.0	36.0
42	34.583473886158345	36.0	36.0	36.0	32.0	36.0
43	34.57872277334784	36.0	36.0	36.0	32.0	36.0
44	34.54401356441593	36.0	36.0	36.0	32.0	36.0
45	34.51044587109123	36.0	36.0	36.0	32.0	36.0
46	34.51244352340386	36.0	36.0	36.0	32.0	36.0
47	34.48592223647878	36.0	36.0	36.0	32.0	36.0
48	34.45364933844488	36.0	36.0	36.0	32.0	36.0
49	34.44372238875518	36.0	36.0	36.0	32.0	36.0
50	33.91585335537588	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	58.0
22	150.0
23	474.0
24	1340.0
25	2931.0
26	6075.0
27	11057.0
28	18325.0
29	28002.0
30	40278.0
31	57440.0
32	84637.0
33	139510.0
34	309481.0
35	1094334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90876965693016	17.801991601224863	11.527940449306039	26.76129829253894
2	16.80634281124462	19.927191893562597	35.585171047768966	27.681294247423814
3	18.689889543072738	23.029631128375176	28.445738320142823	29.834741008409267
4	12.867432917731033	15.15046794972195	34.782339698445405	37.19975943410161
5	15.052622308826791	35.872908289699716	32.89081024198124	16.183659159492247
6	35.75518576218093	34.19640543003576	15.996533070991223	14.051875736792088
7	31.219166346636733	29.828889422736598	20.169800730504324	18.782143500122345
8	28.830292078617433	32.11566094756943	19.056789286697665	19.99725768711548
9	27.427643628637327	14.002907297562128	18.47672322594094	40.0927258478596
10	16.597570043241593	26.022988608253918	30.627120136714332	26.752321211790164
11	38.287537079748915	20.748829779288403	21.3792830523949	19.58435008856779
12	25.02321490480496	23.281623270865822	27.690058446936806	24.005103377392416
13	29.751976750537594	18.549294188860635	24.97695230939532	26.72177675120645
14	23.91915527845066	19.192957383788915	24.16986510273083	32.71802223502959
15	25.683265091360262	26.557683882669142	21.76978394810563	25.989267077864962
16	26.723114464808656	25.100300651132095	22.995408298060426	25.18117658599882
17	24.77640674519789	25.39900095089142	24.557579095103634	25.26701320880706
18	25.865249879327084	23.942007885821685	25.503677040264066	24.689065194587165
19	26.084244743621614	24.593139981695618	24.751213139023	24.571402135659767
20	26.141822166583246	23.625694356966644	24.370856571462333	25.861626904987776
21	27.239471915260303	23.678756996520832	24.03364126757282	25.048129820646047
22	26.363436720015425	23.65367486647946	24.33362354286759	25.649264870637523
23	24.790452738021056	23.381951791031298	25.12298604430285	26.704609426644804
24	25.300177358528426	24.477985135772357	24.57156934986004	25.65026815583917
25	25.462040704395395	23.779196992819823	24.715986680831566	26.04277562195322
26	25.25703609485727	24.613205685728715	24.999804916766344	25.129953302647667
27	26.008050806362608	23.973332679340018	24.348338392491858	25.670278121805513
28	25.186917606874953	23.869715613235783	25.193940603286542	25.749426176602718
29	25.0244968803404	24.27398381143589	25.19906850542833	25.502450802795373
30	25.03447399429019	24.350400700961927	25.3304988668451	25.28462643790278
31	25.519395175089986	24.177166789476207	24.20330794278599	26.100130092647817
32	25.361990874563713	24.13803866661167	24.192439019768063	26.307531439056554
33	24.976339190660976	23.75099353103997	24.860682702136884	26.41198457616217
34	25.833646395474965	23.973555631607052	24.912965008756448	25.279832964161535
35	26.38924344492466	23.709914575838887	24.919151934166656	24.9816900450698
36	25.07599885402535	24.412102740863695	24.555572524700324	25.956325880410635
37	26.2112160596977	24.04974956886605	24.469791639958842	25.269242731477405
38	25.030182163149778	23.829194038702283	25.049746224582048	26.090877573565884
39	25.68867168383585	23.79251839077513	24.503847598748347	26.014962326640678
40	25.84223005775578	23.983811435890633	24.963073530772427	25.210884975581155
41	24.778078887200643	24.387076348889085	25.44504059403402	25.38980416987625
42	26.16155344221579	24.42665037628769	24.766150940914304	24.64564524058222
43	25.33412184118441	23.295000406887887	25.0714840706179	26.2993936813098
44	24.968424385181255	23.848033505266688	24.93096840431948	26.252573705232578
45	24.88331235724088	24.24817708652666	25.04690358317736	25.821606973055104
46	24.783652693876505	23.899479740884875	25.281895272631605	26.034972292607016
47	24.930299547518374	23.71214409850923	25.67440273874565	25.68315361522675
48	25.75221307994065	24.545929839151086	24.544369173281847	25.157487907626418
49	24.530880561126267	24.55016593222474	24.893345209257426	26.02560829739157
50	24.36734507325654	24.81492174932808	24.987821232413246	25.829911945002138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	30.5
2	32.0
3	128.0
4	224.0
5	265.0
6	306.0
7	275.0
8	244.0
9	346.5
10	449.0
11	677.0
12	905.0
13	1593.5
14	2282.0
15	2925.0
16	3568.0
17	4022.5
18	4477.0
19	4766.0
20	5055.0
21	5647.5
22	6240.0
23	6762.0
24	7284.0
25	8283.0
26	9282.0
27	11309.5
28	13337.0
29	15363.5
30	17390.0
31	19429.5
32	21469.0
33	24898.5
34	28328.0
35	33247.5
36	38167.0
37	46983.0
38	55799.0
39	62691.0
40	69583.0
41	77571.0
42	85559.0
43	88424.5
44	91290.0
45	102157.0
46	113024.0
47	122572.0
48	132120.0
49	137008.0
50	141896.0
51	137035.5
52	132175.0
53	131351.0
54	130527.0
55	133479.5
56	136432.0
57	133080.5
58	129729.0
59	120049.0
60	110369.0
61	97747.0
62	85125.0
63	74877.5
64	64630.0
65	55246.0
66	45862.0
67	40129.0
68	34396.0
69	31355.5
70	28315.0
71	22112.0
72	15909.0
73	14393.5
74	12878.0
75	9367.0
76	5856.0
77	5358.5
78	4861.0
79	4195.5
80	3530.0
81	2819.5
82	2109.0
83	1822.0
84	1535.0
85	1239.0
86	943.0
87	672.0
88	401.0
89	262.5
90	124.0
91	78.0
92	32.0
93	23.0
94	14.0
95	10.5
96	7.0
97	6.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015216492225097068
2	0.004180355006894799
3	7.245948678617652E-4
4	1.6721420027579196E-4
5	0.0
6	6.131187343445705E-4
7	1.6721420027579196E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1794106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.80222023203684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43318548410144	44.62719365096033
2	11.117450664116122	12.185219594273917
3	3.0462933712645737	5.008309206703053
4	1.3187488778967318	2.8908146574899263
5	0.727622819963523	1.9937673012748336
6	0.46816757593282043	1.5393973561061554
7	0.3259563018119931	1.250419032654478
8	0.2399225717707083	1.0518631693452016
9	0.18279636746257055	0.9015882108570112
>10	0.9738929038107439	9.514453998634634
>50	0.07469962346824469	2.8768048414940814
>100	0.07601936911549236	8.90556830293605
>500	0.011663247640925658	4.389652957916728
>1k	0.003580821644143842	2.8649477193535606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2406	0.13410578862118513	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2285	0.12736148254339488	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2037	0.11353844198726275	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2035	0.11342696585374555	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2011	0.1120892522515392	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1919	0.10696135010974826	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1832	0.1021121383017503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5738066758597315E-5
2	0.0	0.0	0.0	0.0	5.5738066758597315E-5
3	0.0	0.0	0.0	0.0	5.5738066758597315E-5
4	0.0	0.0	0.0	5.5738066758597315E-5	5.5738066758597315E-5
5	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
6	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
7	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
8	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
9	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
10	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
11	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	5.5738066758597315E-5
12	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	1.1147613351719463E-4
13	1.1147613351719463E-4	0.0	0.0	1.6721420027579196E-4	2.786903337929866E-4
14	1.1147613351719463E-4	0.0	0.0	2.2295226703438926E-4	2.786903337929866E-4
15	1.1147613351719463E-4	0.0	0.0	2.786903337929866E-4	3.901664673101812E-4
16	1.1147613351719463E-4	0.0	0.0	3.3442840055158393E-4	3.901664673101812E-4
17	1.1147613351719463E-4	0.0	0.0	3.3442840055158393E-4	3.901664673101812E-4
18	1.1147613351719463E-4	0.0	0.0	3.901664673101812E-4	4.459045340687785E-4
19	1.1147613351719463E-4	0.0	0.0	5.016426008273759E-4	4.459045340687785E-4
20	1.1147613351719463E-4	0.0	0.0	5.573806675859732E-4	6.688568011031679E-4
21	1.1147613351719463E-4	0.0	0.0	6.131187343445705E-4	6.688568011031679E-4
22	1.1147613351719463E-4	0.0	0.0	0.0013377136022063357	7.245948678617652E-4
23	1.1147613351719463E-4	0.0	0.0	0.0017278800695165169	7.245948678617652E-4
24	1.1147613351719463E-4	0.0	0.0	0.002619689137654074	7.245948678617652E-4
25	1.1147613351719463E-4	0.0	0.0	0.00312133173848145	7.245948678617652E-4
26	1.1147613351719463E-4	0.0	0.0	0.004124616940136202	7.803329346203624E-4
27	1.1147613351719463E-4	0.0	0.0	0.0059639731431699134	7.803329346203624E-4
28	1.1147613351719463E-4	0.0	0.0	0.011370565618753853	7.803329346203624E-4
29	1.1147613351719463E-4	0.0	0.0	0.02591820104274775	7.803329346203624E-4
30	1.1147613351719463E-4	0.0	0.0	0.055013471890735556	7.803329346203624E-4
31	1.1147613351719463E-4	0.0	0.0	0.12440736500518922	8.360710013789597E-4
32	1.1147613351719463E-4	0.0	0.0	0.2057292044059827	8.360710013789597E-4
33	1.1147613351719463E-4	0.0	0.0	0.28866744774277553	8.360710013789597E-4
34	1.1147613351719463E-4	0.0	0.0	0.3870451355716998	8.91809068137557E-4
35	1.1147613351719463E-4	0.0	0.0	0.49723929355344665	8.91809068137557E-4
36	1.1147613351719463E-4	0.0	0.0	0.6555911412146217	8.91809068137557E-4
37	1.1147613351719463E-4	0.0	0.0	0.8692908891670837	8.91809068137557E-4
38	1.1147613351719463E-4	0.0	0.0	1.1561747187735842	8.91809068137557E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	60	2.8737623E-4	21.999735	5
GTTATAT	215	0.0	20.473999	1
TAGACGG	55	0.004484756	19.99976	5
CGCAATA	735	0.0	18.5576	36
ATACGAA	740	0.0	18.134916	40
GTATAGA	240	0.0	17.424227	1
ATACCGT	525	0.0	17.180746	6
GTATCAA	3400	0.0	17.154503	1
CAATACG	810	0.0	16.839304	38
GTATTAC	170	2.4556357E-9	16.830832	1
CTAGCGG	785	0.0	16.815084	29
TCTAGCG	800	0.0	16.774797	28
CGAATGC	800	0.0	16.774797	43
TACGAAT	825	0.0	16.533134	41
GCCGGTC	865	0.0	16.531591	8
GTATTAT	120	6.383618E-6	16.507162	1
TTACGCT	80	0.0019890154	16.499802	4
CGCCGGT	815	0.0	16.466059	7
TACCGTC	535	0.0	16.4484	7
ACCGTCG	485	0.0	16.3297	8
>>END_MODULE