FastQCFastQC Report
Thu 2 Feb 2017
SRR4062600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708919
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG83141.1727715013986082No Hit
TCCATGTACTCTGCGTTGATACCAC69120.9750056071286No Hit
GAGTACATGGAAGCAGTGGTATCAA36080.5089438990914336No Hit
CATGTACTCTGCGTTGATACCACTG30070.4241669358558594No Hit
GCTTCCATGTACTCTGCGTTGATAC22730.32062901403404337No Hit
GTATCAACGCAGAGTACTTTTTTTT20190.28479981492949125No Hit
CATGGAAGCAGTGGTATCAACGCAG19850.28000378040368507No Hit
GCGTTGATACCACTGCTTCCATGTA18970.26759051457218663No Hit
CCCATGTACTCTGCGTTGATACCAC18590.26223024069040324No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18340.2587037447155458No Hit
GTACATGGGAAGCAGTGGTATCAAC16800.23698052951042362No Hit
TATCAACGCAGAGTACTTTTTTTTT14020.19776589427000826No Hit
GTATCAACGCAGAGTACATGGAAGC13110.18492944892152702No Hit
ACGCAGAGTACATGGAAGCAGTGGT12080.17040028550511413No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11950.16856650759818823No Hit
CAGTGGTATCAACGCAGAGTACATG11460.16165457548746753No Hit
ACTCTGCGTTGATACCACTGCTTCC11430.16123139597048464No Hit
TATCAACGCAGAGTACATGGAAGCA10110.14261149722323707No Hit
GTACATGGGGTGGTATCAACGCAAA8590.1211704016961035No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8360.11792602539923462No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8050.11355317039041131No Hit
GTATCAACGCAGAGTACATGGGAAG8040.11341211055141702No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7510.10593593908471914No Hit
GAATAGGACCGCGGTTCTATTTTGT7140.10071672504193004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA453.5499655E-516.8802932
CGAACGA350.002166799716.28777316
GCACGCC350.002166799716.28777315
GCAATAG350.002173967416.2797243
AAACGCT508.693886E-515.201920519
CGGTCCA1200.015.0446310
TCGCGTA456.749423E-414.7796479
GCGTTAT456.7965634E-414.7660881
TTCGGAA1058.731149E-1114.4780218
ATCCCCG801.2830424E-714.251801512
GTCAATA400.005270924414.251801513
CACCGTG400.005270924414.25180157
TCCAACG602.5613115E-514.25180118
GTTTTCG1101.8553692E-1013.81992915
CGTCGTA852.685556E-713.41440910
TACCGTC852.687866E-713.413467
GCGTAAC500.001495918413.30262111
CGTTATT500.001503901313.2932292
ATCGCCA1102.730303E-912.95618316
CCGTCGT905.38279E-712.6682689
Produced by FastQC (version 0.11.5)