##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062600_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708919
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.107216762422787	32.0	32.0	32.0	32.0	32.0
2	30.844251599971223	32.0	32.0	32.0	32.0	32.0
3	30.852599521242908	32.0	32.0	32.0	32.0	32.0
4	30.883050108686607	32.0	32.0	32.0	32.0	32.0
5	30.735141814509134	32.0	32.0	32.0	32.0	32.0
6	34.34953076444558	36.0	36.0	36.0	32.0	36.0
7	34.213921477630024	36.0	36.0	36.0	32.0	36.0
8	34.204246183273405	36.0	36.0	36.0	32.0	36.0
9	34.327745482911304	36.0	36.0	36.0	32.0	36.0
10	34.0698359050893	36.0	36.0	36.0	32.0	36.0
11	34.36967834124914	36.0	36.0	36.0	32.0	36.0
12	34.15566658532216	36.0	36.0	36.0	32.0	36.0
13	34.25883916216098	36.0	36.0	36.0	32.0	36.0
14	34.16952571450335	36.0	36.0	36.0	32.0	36.0
15	34.12764222710916	36.0	36.0	36.0	32.0	36.0
16	34.11219053234573	36.0	36.0	36.0	32.0	36.0
17	34.04660899200049	36.0	36.0	36.0	32.0	36.0
18	34.06037220049117	36.0	36.0	36.0	32.0	36.0
19	34.03525085376467	36.0	36.0	36.0	32.0	36.0
20	34.01936187350036	36.0	36.0	36.0	32.0	36.0
21	33.956612814722135	36.0	36.0	36.0	32.0	36.0
22	33.90880763528696	36.0	36.0	36.0	32.0	36.0
23	33.8366244944768	36.0	36.0	36.0	32.0	36.0
24	33.815758923092766	36.0	36.0	36.0	32.0	36.0
25	33.28397602546976	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	26.0
5	76.0
6	133.0
7	30.0
8	94.0
9	136.0
10	89.0
11	31.0
12	52.0
13	48.0
14	136.0
15	211.0
16	265.0
17	360.0
18	528.0
19	692.0
20	1090.0
21	1541.0
22	2183.0
23	3270.0
24	4301.0
25	5974.0
26	8135.0
27	10325.0
28	14085.0
29	19142.0
30	25048.0
31	35287.0
32	50848.0
33	73140.0
34	161510.0
35	290132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.48603201428751	17.160103883499254	11.24492326530497	25.108940836908268
2	15.17771875057307	20.839017013659173	38.0026266008935	25.980637634874267
3	19.41096477863922	25.455078940537856	28.07693175790816	27.057024522914762
4	12.050681199678905	15.748924625752833	36.003205313597334	36.19718886097093
5	13.551565406119378	37.47590434554368	34.136239582069166	14.836290666267777
6	33.4397430360918	35.03800548136983	17.894047548484153	13.62820393405421
7	28.996071578299524	30.870223749235983	21.93112346085059	18.2025812116139
8	28.087964963107915	33.34716963017555	20.650108290200798	17.91475711651574
9	27.206105576775087	14.835817312783728	18.673124307227425	39.284952803213756
10	15.345536923604921	27.560755490838833	32.72555480387491	24.36815278168134
11	36.781924134015405	21.608021038418844	21.739934862253154	19.870119965312597
12	23.44693738028917	24.308007842295286	30.080400476865794	22.164654300549753
13	30.493101271275613	20.27134481779701	25.22114207186429	24.014411839063087
14	23.552608348693177	19.91101883434673	25.411749751064594	31.124623065895495
15	25.137696305502207	27.42613828945882	24.234549768387755	23.201615636651226
16	25.40612603146762	25.859032641192613	25.708204995671473	23.0266363316683
17	23.248843955944622	26.183938826414018	27.398597210805377	23.168620006835987
18	24.695851890146493	24.269415970653753	28.980268687116943	22.05446345208281
19	24.268580863959503	27.338754444025408	25.616796120756018	22.77586857125907
20	24.221025187629053	24.241927784352992	28.290817263285156	23.2462297647328
21	25.14781198710724	26.021548205210777	23.951474165035297	24.87916564264669
22	23.763293814491245	26.618567828121293	25.517907799414562	24.1002305579729
23	22.46804062548557	25.990846552625257	25.738279208396307	25.802833613492858
24	22.912785104973437	27.23178908976878	25.87136498663024	23.984060818627544
25	24.52174871044469	25.424013694992624	25.201978542251652	24.85225905231103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	344.0
3	559.0
4	559.0
5	559.0
6	1592.5
7	2626.0
8	2626.0
9	2626.0
10	2532.5
11	2439.0
12	2439.0
13	2439.0
14	2380.0
15	2321.0
16	2321.0
17	2321.0
18	3720.0
19	5119.0
20	5119.0
21	5119.0
22	8336.0
23	11553.0
24	11553.0
25	11553.0
26	17367.0
27	23181.0
28	23181.0
29	23181.0
30	29553.0
31	35925.0
32	35925.0
33	35925.0
34	42199.5
35	48474.0
36	48474.0
37	48474.0
38	53794.0
39	59114.0
40	59114.0
41	59114.0
42	68861.5
43	78609.0
44	78609.0
45	78609.0
46	100447.5
47	122286.0
48	122286.0
49	122286.0
50	107837.0
51	93388.0
52	93388.0
53	93388.0
54	80402.0
55	67416.0
56	67416.0
57	67416.0
58	60985.0
59	54554.0
60	54554.0
61	54554.0
62	48394.5
63	42235.0
64	42235.0
65	42235.0
66	35167.5
67	28100.0
68	28100.0
69	28100.0
70	21108.5
71	14117.0
72	14117.0
73	14117.0
74	10935.0
75	7753.0
76	7753.0
77	7753.0
78	6493.0
79	5233.0
80	5233.0
81	5233.0
82	3644.5
83	2056.0
84	2056.0
85	2056.0
86	1521.0
87	986.0
88	986.0
89	986.0
90	664.5
91	343.0
92	343.0
93	343.0
94	203.5
95	64.0
96	64.0
97	64.0
98	201.5
99	339.0
100	339.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0067708722717263895
2	0.002539077101897396
3	0.004372855008823293
4	0.013541744543452779
5	0.040202054113375435
6	0.047537165741079024
7	0.06940144078519549
8	0.08985511743936896
9	0.10071672504193004
10	0.1235684189590066
11	0.12427371815397811
12	0.1358406249515107
13	0.1255432567049268
14	0.127518094450847
15	0.11834920491621752
16	0.11651542700929161
17	0.1270949149338641
18	0.13513532575653917
19	0.1334426076886076
20	0.1231452394420237
21	0.13048035106972727
22	0.15107508756289506
23	0.13950818076536248
24	0.13795652253642518
25	0.13019823139173867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	708919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22904938406129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29635271284505	42.74098523912033
2	12.559184119348727	13.370268634246631
3	3.3789316634110866	5.3957196113123125
4	1.290975566699425	2.748696087738408
5	0.6223531690680761	1.6563633785325833
6	0.36542963081672336	1.167088311908559
7	0.2363945609215412	0.8808140430191341
8	0.16319133608633743	0.6949215750072479
9	0.11543329636128283	0.5529964169321736
>10	0.7564662288552906	7.844948467635268
>50	0.10737877051775746	4.017762322434828
>100	0.09518258299677648	10.052373000704147
>500	0.007953976294981468	2.6951124850029045
>1k	0.004242120690656783	4.033142619071739
>5k	5.302650863320979E-4	2.1488078073338337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8314	1.1727715013986082	No Hit
TCCATGTACTCTGCGTTGATACCAC	6912	0.9750056071286	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3608	0.5089438990914336	No Hit
CATGTACTCTGCGTTGATACCACTG	3007	0.4241669358558594	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2273	0.32062901403404337	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2019	0.28479981492949125	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1985	0.28000378040368507	No Hit
GCGTTGATACCACTGCTTCCATGTA	1897	0.26759051457218663	No Hit
CCCATGTACTCTGCGTTGATACCAC	1859	0.26223024069040324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1834	0.2587037447155458	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1680	0.23698052951042362	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1402	0.19776589427000826	No Hit
GTATCAACGCAGAGTACATGGAAGC	1311	0.18492944892152702	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1208	0.17040028550511413	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1195	0.16856650759818823	No Hit
CAGTGGTATCAACGCAGAGTACATG	1146	0.16165457548746753	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1143	0.16123139597048464	No Hit
TATCAACGCAGAGTACATGGAAGCA	1011	0.14261149722323707	No Hit
GTACATGGGGTGGTATCAACGCAAA	859	0.1211704016961035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	836	0.11792602539923462	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	805	0.11355317039041131	No Hit
GTATCAACGCAGAGTACATGGGAAG	804	0.11341211055141702	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	751	0.10593593908471914	No Hit
GAATAGGACCGCGGTTCTATTTTGT	714	0.10071672504193004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4105983899429978E-4
2	0.0	0.0	0.0	0.0	1.4105983899429978E-4
3	0.0	0.0	0.0	0.0	1.4105983899429978E-4
4	0.0	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
5	0.0	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
6	0.0	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
7	0.0	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
8	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
9	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
10	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
11	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	1.4105983899429978E-4
12	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	2.8211967798859956E-4
13	1.4105983899429978E-4	0.0	0.0	1.4105983899429978E-4	2.8211967798859956E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAATA	45	3.5499655E-5	16.880293	2
CGAACGA	35	0.0021667997	16.287773	16
GCACGCC	35	0.0021667997	16.287773	15
GCAATAG	35	0.0021739674	16.279724	3
AAACGCT	50	8.693886E-5	15.2019205	19
CGGTCCA	120	0.0	15.04463	10
TCGCGTA	45	6.749423E-4	14.779647	9
GCGTTAT	45	6.7965634E-4	14.766088	1
TTCGGAA	105	8.731149E-11	14.47802	18
ATCCCCG	80	1.2830424E-7	14.2518015	12
GTCAATA	40	0.0052709244	14.2518015	13
CACCGTG	40	0.0052709244	14.2518015	7
TCCAACG	60	2.5613115E-5	14.251801	18
GTTTTCG	110	1.8553692E-10	13.819929	15
CGTCGTA	85	2.685556E-7	13.414409	10
TACCGTC	85	2.687866E-7	13.41346	7
GCGTAAC	50	0.0014959184	13.302621	11
CGTTATT	50	0.0015039013	13.293229	2
ATCGCCA	110	2.730303E-9	12.956183	16
CCGTCGT	90	5.38279E-7	12.668268	9
>>END_MODULE