FastQCFastQC Report
Thu 2 Feb 2017
SRR4062600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708919
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35540.5013266677857414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32310.45576433979058256No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA14500.2045367665417347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13180.1859168677944871No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG8560.12074722217912061No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8400.11849026475521181No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC8360.11792602539923462No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG8330.11750284588225171No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA7470.10537169972874193No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7430.10480746037276473No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7370.10396110133879892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA401.8190762E-532.99984440
CGATTAA602.9425973E-729.3331941
AACGATT508.336923E-526.39987439
GGTTCGT508.336923E-526.39987426
TCGTATA609.745916E-625.66654244
GCGCACT551.592764E-423.99988432
TGGTTCG651.812087E-523.69219425
TGTAGGA5800.023.5187872
CTACGCA500.002579466421.9998954
GTCGTAT904.0770647E-721.99989543
GTAAGAC803.6122037E-621.9998953
CTGTAGG5550.021.8047731
GTAGGAC6000.021.2665633
GGCGTTA1351.1277734E-1021.18508342
GCGGCGT1351.1277734E-1021.18508340
TAGGACG3650.021.0957894
ATAACGC2250.020.5332343
TTAACGG654.9295573E-420.30759435
GATTTCG2200.019.99990341
CGCAATA2000.019.79990636
Produced by FastQC (version 0.11.5)