##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708919
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18143821790642	32.0	32.0	32.0	32.0	32.0
2	31.37783865293496	32.0	32.0	32.0	32.0	32.0
3	31.480197314502785	32.0	32.0	32.0	32.0	32.0
4	31.600807708638083	32.0	32.0	32.0	32.0	32.0
5	31.527161777297547	32.0	32.0	32.0	32.0	32.0
6	35.11988252536609	36.0	36.0	36.0	36.0	36.0
7	35.14152110466781	36.0	36.0	36.0	36.0	36.0
8	35.08144371924014	36.0	36.0	36.0	36.0	36.0
9	35.19544687051694	36.0	36.0	36.0	36.0	36.0
10	35.08319709303884	36.0	36.0	36.0	36.0	36.0
11	35.211258267869816	36.0	36.0	36.0	36.0	36.0
12	35.11656056615777	36.0	36.0	36.0	36.0	36.0
13	35.161273713922185	36.0	36.0	36.0	36.0	36.0
14	35.125268190018886	36.0	36.0	36.0	36.0	36.0
15	35.07949004047007	36.0	36.0	36.0	36.0	36.0
16	35.0817991900344	36.0	36.0	36.0	36.0	36.0
17	35.06015496833912	36.0	36.0	36.0	36.0	36.0
18	35.07048478034867	36.0	36.0	36.0	36.0	36.0
19	35.03586305346591	36.0	36.0	36.0	36.0	36.0
20	35.023204343514564	36.0	36.0	36.0	36.0	36.0
21	34.983287230275955	36.0	36.0	36.0	36.0	36.0
22	34.955351739761525	36.0	36.0	36.0	36.0	36.0
23	34.88498262848083	36.0	36.0	36.0	32.0	36.0
24	34.860542600776675	36.0	36.0	36.0	32.0	36.0
25	34.827781453170246	36.0	36.0	36.0	32.0	36.0
26	34.772629877320256	36.0	36.0	36.0	32.0	36.0
27	34.729864765932355	36.0	36.0	36.0	32.0	36.0
28	34.689633089252794	36.0	36.0	36.0	32.0	36.0
29	34.65272619297832	36.0	36.0	36.0	32.0	36.0
30	34.61205582019949	36.0	36.0	36.0	32.0	36.0
31	34.594221624755434	36.0	36.0	36.0	32.0	36.0
32	34.55556135468227	36.0	36.0	36.0	32.0	36.0
33	34.513178515458044	36.0	36.0	36.0	32.0	36.0
34	34.4708535107678	36.0	36.0	36.0	32.0	36.0
35	34.38622889215834	36.0	36.0	36.0	32.0	36.0
36	34.34441029229009	36.0	36.0	36.0	32.0	36.0
37	34.27914190478743	36.0	36.0	36.0	32.0	36.0
38	34.19252552125137	36.0	36.0	36.0	32.0	36.0
39	34.04567799706314	36.0	36.0	36.0	32.0	36.0
40	33.96732066710019	36.0	36.0	36.0	32.0	36.0
41	33.965172325752306	36.0	36.0	36.0	32.0	36.0
42	33.81839533148357	36.0	36.0	36.0	32.0	36.0
43	33.84684145861516	36.0	36.0	36.0	32.0	36.0
44	33.8231180148931	36.0	36.0	36.0	32.0	36.0
45	33.6351120508831	36.0	36.0	36.0	27.0	36.0
46	33.73975870303941	36.0	36.0	36.0	27.0	36.0
47	33.64270812321295	36.0	36.0	36.0	27.0	36.0
48	33.628720629578275	36.0	36.0	36.0	27.0	36.0
49	33.681716811088435	36.0	36.0	36.0	27.0	36.0
50	33.081723017721345	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	18.0
22	75.0
23	214.0
24	694.0
25	1614.0
26	3577.0
27	6565.0
28	10911.0
29	15855.0
30	21485.0
31	29508.0
32	42993.0
33	59453.0
34	122606.0
35	393346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88207750140023	17.6695404852902	11.770241234288875	25.6781407790207
2	14.688473344468017	21.335992867783144	37.53568929716066	26.439844490588182
3	18.748933228948463	25.81987967527842	28.559700387211446	26.871486708561676
4	11.95499614200252	16.272003633711705	36.08941526299976	35.683584961286016
5	13.289106371813988	37.70966781818515	34.09571474315119	14.90551106684967
6	33.79516389765812	35.23482264521426	17.450255177608696	13.519758279518928
7	28.779310471295027	31.081124924004012	21.816737878375385	18.322826726325577
8	28.094182833299712	33.42568050792827	20.59459543332877	17.885541225443248
9	27.144850116868074	14.399952603894098	18.650226612631343	39.80497066660648
10	15.35140121791065	27.56224618045221	32.589336722531066	24.497015879106076
11	36.96670564620217	21.266886625975605	21.899963183382024	19.8664445444402
12	23.38080937314418	24.30982947276064	30.151258465353585	22.15810268874159
13	30.530991551926245	20.00679908423953	25.395849173177755	24.06636019065648
14	23.475178405431368	19.765586759559273	25.498963915482587	31.26027091952677
15	25.20513627085746	27.30170865782974	24.207843209167763	23.285311862145043
16	25.499669214677557	25.739894120484852	25.664144986944915	23.09629167789268
17	23.28037476778024	26.192978323334543	27.348117344858863	23.178529564026356
18	24.86066814403338	24.14126296516245	28.96198296279265	22.036085928011524
19	24.48361519440162	27.135399107655463	25.69461391216768	22.686371785775243
20	24.269627418647264	24.027427675094053	28.393652871484615	23.30929203477407
21	25.294145029262864	25.6421396520618	24.215319380634458	24.848395938040877
22	23.81795381418752	26.340385855083586	25.77854451636929	24.06311581435961
23	22.570279538282936	25.65229596046939	25.982093864038063	25.795330637209613
24	23.16188450302503	26.785570706949596	25.957267332375068	24.09527745765031
25	24.783085232586515	24.892688727485087	25.190889227119033	25.13333681280936
26	22.68594860625826	25.74469015501066	26.630404884055864	24.938956354675216
27	24.851922434015734	26.21089292288682	26.035696602855896	22.901488040241553
28	23.44837703602245	24.723981160047902	26.440397280930544	25.387244522999104
29	22.875815149544586	26.098609291047353	27.274625168742833	23.750950390665224
30	24.582639201375617	25.094545357085927	26.919436494155185	23.403378947383267
31	23.199829599714494	25.74045835984083	25.978708427902202	25.081003612542474
32	23.098830754994577	26.290168552401617	26.242490326821542	24.368510365782267
33	22.73348577199934	25.513069899382018	26.767232927880336	24.986211400738306
34	22.885407218596203	26.344476590414416	27.732928585635314	23.037187605354067
35	24.94530404742996	25.694896031845673	26.499924532986135	22.85987538773823
36	23.4520445918363	26.63618833745463	26.379177310807016	23.53258975990205
37	24.80720646505454	26.0702562634095	26.31302024631869	22.809517025217268
38	23.675624436642266	26.205814768683023	25.44550223650375	24.67305855817096
39	23.854629372326034	25.487820188202036	26.50683646509686	24.150713974375073
40	25.204007792145504	25.559337526572147	25.609413769415124	23.627240911867222
41	22.847462121906734	25.789406123971848	27.165162733683257	24.19796902043816
42	25.43788500519806	26.391026337282536	26.29129703111357	21.879791626405837
43	24.00937201570278	24.79140776308718	25.963897144807802	25.23532307640224
44	23.39547959639959	25.627751548484383	27.28379405827746	23.692974796838566
45	24.207702149328767	26.003817079243184	25.912833483091863	23.875647288336186
46	23.168579678580144	25.298166075859374	27.1913355160054	24.341918729555083
47	24.406455462471737	24.7809693350016	27.581853498072416	23.230721704454247
48	23.70679866106001	27.941415027668885	24.86560523839818	23.486181072872924
49	22.838011112694115	25.689958937480867	27.74435443259385	23.727675517231166
50	22.730869763315027	28.14363317567501	24.422040944144378	24.703456116865585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	12.5
2	16.0
3	87.5
4	159.0
5	171.0
6	183.0
7	167.0
8	151.0
9	223.0
10	295.0
11	425.0
12	555.0
13	960.0
14	1365.0
15	1827.0
16	2289.0
17	2661.5
18	3034.0
19	3332.0
20	3630.0
21	4126.0
22	4622.0
23	5014.0
24	5406.0
25	5639.0
26	5872.0
27	7177.0
28	8482.0
29	9884.0
30	11286.0
31	12698.0
32	14110.0
33	16858.5
34	19607.0
35	22545.5
36	25484.0
37	28460.0
38	31436.0
39	32713.0
40	33990.0
41	36221.0
42	38452.0
43	39722.5
44	40993.0
45	47825.5
46	54658.0
47	59672.0
48	64686.0
49	61828.0
50	58970.0
51	52544.5
52	46119.0
53	42857.0
54	39595.0
55	40140.0
56	40685.0
57	39209.0
58	37733.0
59	34463.5
60	31194.0
61	27243.5
62	23293.0
63	20097.0
64	16901.0
65	14636.5
66	12372.0
67	10989.0
68	9606.0
69	8897.5
70	8189.0
71	6289.5
72	4390.0
73	4082.0
74	3774.0
75	2690.0
76	1606.0
77	1475.0
78	1344.0
79	1128.0
80	912.0
81	751.5
82	591.0
83	504.0
84	417.0
85	348.0
86	279.0
87	195.5
88	112.0
89	76.5
90	41.0
91	25.5
92	10.0
93	8.0
94	6.0
95	5.0
96	4.0
97	3.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014952342933395774
2	0.0032443762968688947
3	5.64239355977199E-4
4	2.821196779885995E-4
5	0.0
6	1.4105983899429975E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.821196779885995E-4
47	0.0
48	0.0
49	0.0
50	2.821196779885995E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	708919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.663848473020344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34579642401192	48.53403271759407
2	12.063481497941138	14.395074643004888
3	3.1544122428534536	5.646131222371461
4	1.218208291678175	2.907319796930543
5	0.588169607792226	1.7546231177875589
6	0.32067126761619047	1.1479489152422244
7	0.23196199023862585	0.9687821525968315
8	0.14501920782188485	0.6921923232929906
9	0.10925805083194841	0.5866880210365432
>10	0.6516419612443581	7.474831174496679
>50	0.08889876278011218	3.7336746649883588
>100	0.07653828199206286	8.798302504171634
>500	0.004991627086303625	2.005602830673513
>1k	9.507861116768811E-4	1.3547959158127612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3554	0.5013266677857414	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3231	0.45576433979058256	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1450	0.2045367665417347	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1318	0.1859168677944871	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	856	0.12074722217912061	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	840	0.11849026475521181	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	836	0.11792602539923462	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	833	0.11750284588225171	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	747	0.10537169972874193	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	743	0.10480746037276473	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	737	0.10396110133879892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.2317951698289934E-4	1.4105983899429978E-4
11	0.0	0.0	0.0	4.2317951698289934E-4	1.4105983899429978E-4
12	0.0	0.0	0.0	4.2317951698289934E-4	7.052991949714989E-4
13	0.0	0.0	0.0	4.2317951698289934E-4	7.052991949714989E-4
14	0.0	0.0	0.0	4.2317951698289934E-4	7.052991949714989E-4
15	0.0	0.0	0.0	7.052991949714989E-4	7.052991949714989E-4
16	0.0	0.0	0.0	7.052991949714989E-4	7.052991949714989E-4
17	0.0	0.0	0.0	0.0011284787119543982	7.052991949714989E-4
18	0.0	0.0	0.0	0.0011284787119543982	8.463590339657987E-4
19	0.0	0.0	0.0	0.0014105983899429977	8.463590339657987E-4
20	0.0	0.0	0.0	0.0021158975849144964	8.463590339657987E-4
21	0.0	0.0	0.0	0.002398017262903096	8.463590339657987E-4
22	0.0	0.0	0.0	0.0033854361358631947	8.463590339657987E-4
23	0.0	0.0	0.0	0.00620663291574919	8.463590339657987E-4
24	0.0	0.0	0.0	0.010579487924572483	8.463590339657987E-4
25	0.0	0.0	0.0	0.01213114615350978	8.463590339657987E-4
26	0.0	0.0	0.0	0.015375522450378674	0.0011284787119543982
27	0.0	0.0	0.0	0.021158975849144966	0.0011284787119543982
28	0.0	0.0	0.0	0.030186805544780152	0.0011284787119543982
29	0.0	0.0	0.0	0.04260007137627853	0.0011284787119543982
30	0.0	0.0	0.0	0.0641822267424064	0.0011284787119543982
31	0.0	0.0	0.0	0.10551275956773623	0.0011284787119543982
32	0.0	0.0	0.0	0.15728172047864425	0.0011284787119543982
33	0.0	0.0	0.0	0.20608842477067196	0.0011284787119543982
34	0.0	0.0	0.0	0.27492562619989025	0.0011284787119543982
35	0.0	0.0	0.0	0.34263434891715416	0.0011284787119543982
36	0.0	0.0	0.0	0.4488524076798619	0.0011284787119543982
37	0.0	0.0	0.0	0.5955546402339337	0.0011284787119543982
38	0.0	0.0	0.0	0.7612999510522359	0.0011284787119543982
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	40	1.8190762E-5	32.999844	40
CGATTAA	60	2.9425973E-7	29.33319	41
AACGATT	50	8.336923E-5	26.399874	39
GGTTCGT	50	8.336923E-5	26.399874	26
TCGTATA	60	9.745916E-6	25.666542	44
GCGCACT	55	1.592764E-4	23.999884	32
TGGTTCG	65	1.812087E-5	23.692194	25
TGTAGGA	580	0.0	23.518787	2
CTACGCA	50	0.0025794664	21.999895	4
GTCGTAT	90	4.0770647E-7	21.999895	43
GTAAGAC	80	3.6122037E-6	21.999895	3
CTGTAGG	555	0.0	21.804773	1
GTAGGAC	600	0.0	21.266563	3
GGCGTTA	135	1.1277734E-10	21.185083	42
GCGGCGT	135	1.1277734E-10	21.185083	40
TAGGACG	365	0.0	21.095789	4
ATAACGC	225	0.0	20.533234	3
TTAACGG	65	4.9295573E-4	20.307594	35
GATTTCG	220	0.0	19.999903	41
CGCAATA	200	0.0	19.799906	36
>>END_MODULE