FastQCFastQC Report
Thu 2 Feb 2017
SRR4062599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9211
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1151.2485072196287048No Hit
TATCAACGCAGAGTACTTTTTTTTT961.0422321137770059No Hit
GGTATCAACGCAGAGTACTTTTTTT560.6079687330365867No Hit
GTACTTTTTTTTTTTTTTTTTTTTT500.5428292259255239No Hit
TCCATGTACTCTGCGTTGATACCAC490.5319726414070134No Hit
GAGTACTTTTTTTTTTTTTTTTTTT450.48854630333297144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT410.44511996525892955No Hit
GTACATGGAAGCAGTGGTATCAACG380.4125502117033981No Hit
CTCCGAGGTCACCCCAACCGAAATT320.34741070459233525No Hit
GTTATAGATTAACCCAATTTTAAGT250.2714146129627619No Hit
CCATTGGGATGTCCTGATCCAACAT240.26055802844425147No Hit
CCATAGGGTCTTCTCGTCTTATTAT240.26055802844425147No Hit
GTATTAGAGGCACTGCCTGCCCAGT230.24970144392574095No Hit
ATCTATAACTTTATAGATGCAACAC220.2388448594072305No Hit
GCAGAGTACTTTTTTTTTTTTTTTT210.22798827488872No Hit
CTGTAGGACGTGGAATATGGCAAGA210.22798827488872No Hit
GTATCAACGCAGAGTACATGGGCAG200.21713169037020952No Hit
CTATAGAACTAGTACCGCAAGGGAA190.20627510585169906No Hit
GAGTACATGGAAGCAGTGGTATCAA190.20627510585169906No Hit
GTGTAGAAGCTATTAATGGTTCGTT190.20627510585169906No Hit
ATCAACGCAGAGTACTTTTTTTTTT180.19541852133318857No Hit
ATCGTAAATAGATAGAAACCGACCT180.19541852133318857No Hit
CATGTACTCTGCGTTGATACCACTG180.19541852133318857No Hit
CTTTAGGACGTGAAATATGGCGAGG170.1845619368146781No Hit
GTATCAACGCAGAGTACATGGGAAG170.1845619368146781No Hit
GATATATTTTGATCAACGGACCAAG170.1845619368146781No Hit
GTATCCTGACCGTGCAAAGGTAGCA170.1845619368146781No Hit
TCCCAATGGTGTAGAAGCTATTAAT160.17370535229616763No Hit
CCAATAAAGAAAGCGTTCAAGCTCA150.16284876777765714No Hit
GATAGAAACCGACCTGGATTGCTCC150.16284876777765714No Hit
CATCTAATATGTTCTACAGTGTGGT150.16284876777765714No Hit
CTGTTAGTATGAGTAACAAGAATTC150.16284876777765714No Hit
GATATACACTGTTCTACAATGCCGG140.15199218325914668No Hit
CCTTTAGGCATTCCGGTGTTGGGTT140.15199218325914668No Hit
CTGAAGGACCTGGAATATGGCGAGA130.1411355987406362No Hit
AAGTTACCCTAGGGATAACAGCGCA130.1411355987406362No Hit
GTCCTACAGTGGACATTTCTAAATT130.1411355987406362No Hit
AGCATGAACGGCTAAACGAGGGTCC130.1411355987406362No Hit
CTTCTACACCATTGGGATGTCCTGA130.1411355987406362No Hit
ATTTAGAAATGTCCACTGTAGGACG130.1411355987406362No Hit
GTTAGTATGAGTAACAAGAATTCCA130.1411355987406362No Hit
GTTATATAATTTAAGCTCCATAGGG130.1411355987406362No Hit
GTACATGGGGTGGTATCAACGCAAA120.13027901422212573No Hit
ATTTAAGAGTTCATATCGACAATTA120.13027901422212573No Hit
GTTCATGCTAGTCCCTAATTAAGGA120.13027901422212573No Hit
CAGTGGTATCAACGCAGAGTACATG120.13027901422212573No Hit
ACCTATAACTTCTCTGTTAACCCAA120.13027901422212573No Hit
CATACTAACAGTGTTGCATCTATAA120.13027901422212573No Hit
AATCTAAACTTACTTTTTGATTTTG120.13027901422212573No Hit
CTGTTAACCCAACACCGGAATGCCT120.13027901422212573No Hit
GTCAGGATACCGCGGCCGTTAAACT120.13027901422212573No Hit
GTTCTACAGTGTGGTTTTTATCATT120.13027901422212573No Hit
CTCTTATCTTTAATCAGTGAAATTG110.11942242970361525No Hit
GATATGTTGATTTTACTTTGACTTG110.11942242970361525No Hit
GTCCTAAAGTGTGTATTTCTCATTT110.11942242970361525No Hit
GTATCAACGCAGAGTACATGGGAGA110.11942242970361525No Hit
GATATACACTGTTCTACAAATCCCG110.11942242970361525No Hit
GGTGATAGCTGGTTACCCAAAAAAT110.11942242970361525No Hit
CTATTATATAAATCAAAACATTTAT110.11942242970361525No Hit
AAATATATCTGGGTCAATAAGATAT110.11942242970361525No Hit
GTTATACGCGTATGCCTGGAGAATT110.11942242970361525No Hit
CTTAAATAGGATTGCGCTGTTATCC110.11942242970361525No Hit
GTATCAACGCAGAGTACATGGGGTG100.10856584518510476No Hit
ACTCTGCGTTGATACCACTGCTTCC100.10856584518510476No Hit
TACTAACAGTGTTGCATCTATAAAG100.10856584518510476No Hit
TATCAACGCAGAGTACATGGGCAGT100.10856584518510476No Hit
ATTTTAAACTTGCTAAAAAAACAAC100.10856584518510476No Hit
GTCCTTCAGTGTGCATTTCTCATTT100.10856584518510476No Hit
ATTTACACCAACTTCCTAAACTTAA100.10856584518510476No Hit
GACCTGGATTGCTCCGGTCTGAACT100.10856584518510476No Hit
ATATAAATCAAAACATTTATCCTAC100.10856584518510476No Hit
CCTTTAATAGTGAATAATTAACAAA100.10856584518510476No Hit
GTACATGGGCAGTGGTATCAACGCA100.10856584518510476No Hit
TTGATATACACTGTTCTACAAATCC100.10856584518510476No Hit
TTGTAGAACAGTGTATATCAATGAG100.10856584518510476No Hit
ATCCTGACCGTGCAAAGGTAGCATA100.10856584518510476No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)