##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9211
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.73455650852242	32.0	32.0	32.0	21.0	32.0
2	30.251329931603518	32.0	32.0	32.0	21.0	32.0
3	30.97915535772446	32.0	32.0	32.0	32.0	32.0
4	31.31712083378569	32.0	32.0	32.0	32.0	32.0
5	31.107263055042882	32.0	32.0	32.0	32.0	32.0
6	34.4686787536641	36.0	36.0	36.0	32.0	36.0
7	34.58213006188253	36.0	36.0	36.0	32.0	36.0
8	34.44490283356856	36.0	36.0	36.0	32.0	36.0
9	34.630876126370644	36.0	36.0	36.0	32.0	36.0
10	34.630224731299535	36.0	36.0	36.0	32.0	36.0
11	34.79285636738682	36.0	36.0	36.0	32.0	36.0
12	34.59559222668548	36.0	36.0	36.0	32.0	36.0
13	34.68135924438172	36.0	36.0	36.0	32.0	36.0
14	34.54934317663663	36.0	36.0	36.0	32.0	36.0
15	34.60938008902399	36.0	36.0	36.0	32.0	36.0
16	34.61893388340028	36.0	36.0	36.0	32.0	36.0
17	34.651937900336556	36.0	36.0	36.0	32.0	36.0
18	34.70046683313429	36.0	36.0	36.0	32.0	36.0
19	34.58766691998697	36.0	36.0	36.0	32.0	36.0
20	34.51590489631962	36.0	36.0	36.0	32.0	36.0
21	34.52958419281294	36.0	36.0	36.0	32.0	36.0
22	34.49864292693518	36.0	36.0	36.0	32.0	36.0
23	34.50059711214852	36.0	36.0	36.0	32.0	36.0
24	34.562371078058845	36.0	36.0	36.0	32.0	36.0
25	34.4679187927478	36.0	36.0	36.0	32.0	36.0
26	34.46107914450114	36.0	36.0	36.0	32.0	36.0
27	34.37259798067528	36.0	36.0	36.0	32.0	36.0
28	34.375854956030835	36.0	36.0	36.0	32.0	36.0
29	34.384214526110085	36.0	36.0	36.0	32.0	36.0
30	34.233850830528716	36.0	36.0	36.0	32.0	36.0
31	34.100640538486594	36.0	36.0	36.0	32.0	36.0
32	34.09792639235697	36.0	36.0	36.0	32.0	36.0
33	34.056128541960696	36.0	36.0	36.0	32.0	36.0
34	34.03550103137553	36.0	36.0	36.0	32.0	36.0
35	33.83356855933123	36.0	36.0	36.0	32.0	36.0
36	33.823146238193466	36.0	36.0	36.0	32.0	36.0
37	33.79730756703941	36.0	36.0	36.0	32.0	36.0
38	33.76245793073499	36.0	36.0	36.0	32.0	36.0
39	33.72326566062317	36.0	36.0	36.0	32.0	36.0
40	33.61383128867658	36.0	36.0	36.0	21.0	36.0
41	33.5532515470633	36.0	36.0	36.0	21.0	36.0
42	33.46943871458039	36.0	36.0	36.0	21.0	36.0
43	33.512213657583324	36.0	36.0	36.0	21.0	36.0
44	33.51666485723592	36.0	36.0	36.0	21.0	36.0
45	33.27792856367387	36.0	36.0	36.0	21.0	36.0
46	33.37411790250787	36.0	36.0	36.0	21.0	36.0
47	33.15579198784062	36.0	36.0	36.0	14.0	36.0
48	33.225925523830206	36.0	36.0	36.0	21.0	36.0
49	33.04679187927478	36.0	36.0	36.0	14.0	36.0
50	32.50016284876778	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	11.0
24	21.0
25	58.0
26	91.0
27	124.0
28	159.0
29	246.0
30	372.0
31	507.0
32	755.0
33	1121.0
34	1983.0
35	3761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28019113814075	24.500434404865334	13.097306689834925	22.122067767158992
2	14.678102269026164	24.231896645315384	40.929323634784495	20.160677450873955
3	19.617848224948432	28.368255346867876	32.7434589078276	19.270437520356097
4	11.659971772880253	19.715557485615026	41.168168494191725	27.456302247312998
5	11.735967864509824	41.363587015524914	35.53360112908479	11.366843990880469
6	26.120942351536208	43.18749321463468	19.205298013245034	11.486266420584085
7	24.720442948648355	36.76039517967647	22.657691890131364	15.861469981543808
8	23.993051785908154	37.62892194115731	22.11486266420584	16.263163608728696
9	26.14265552057323	16.360872869395287	22.53826946042775	34.958202149603736
10	15.188361741396156	29.61676256649658	35.46846162197373	19.726414070133536
11	30.91955270871784	25.132993160351752	27.130604711757684	16.816849419172726
12	22.34285093909456	28.444251438497446	32.58061014004994	16.63228748235805
13	25.33926826620345	24.655303441537292	29.10650309412659	20.898925198132666
14	19.910976006948214	26.555205732276626	28.064270980349583	25.46954728042558
15	22.08229291065031	32.67831940071653	25.6975355553143	19.54185213331886
16	20.855498860058624	31.408099012050812	28.053414395831073	19.682987732059495
17	17.97850396265335	31.679513625013573	28.98708066442297	21.35490174791011
18	19.183584844208013	29.3236347844968	32.56975355553143	18.92302681576376
19	20.996634458799264	31.049831722939963	28.118553902942132	19.83497991531864
20	19.976115514059277	32.59146672456845	28.672239713386166	18.760178047986102
21	22.418847030724134	28.76994897405276	27.32602323309087	21.485180762132234
22	20.55151449354033	32.49375746390186	27.478015416350015	19.476712626207796
23	21.040060796873302	31.84236239279123	27.673433937683207	19.444142872652264
24	20.08468135924438	32.89545109108674	28.053414395831073	18.966453153837804
25	21.59374660731734	32.48290087938335	28.03170122679405	17.891651286505265
26	17.652806427098035	33.601129084789925	29.909890348496365	18.83617413961568
27	19.672131147540984	32.884594506568234	29.063076756052546	18.380197589838236
28	18.21734882206058	32.667462816198025	30.181304961459126	18.933883400282273
29	20.345239387688633	31.42981218108783	27.912278797090433	20.3126696341331
30	20.29095646509608	32.504614048420365	27.95570513516448	19.248724351319073
31	19.454999457170775	31.592660948865486	28.98708066442297	19.965258929540767
32	17.468244490283357	34.04624905004886	29.269351861904248	19.216154597763545
33	19.1727282596895	33.199435457605034	28.878514819237864	18.749321463467595
34	18.8578873086527	33.199435457605034	29.747041580718708	18.195635653023558
35	20.07382477472587	33.11258278145696	27.29345347953534	19.520138964281834
36	18.40191075887526	32.56975355553143	28.98708066442297	20.04125502117034
37	20.117251112799913	31.983497991531863	27.51058516990555	20.388665725762674
38	19.454999457170775	33.123439365975464	28.03170122679405	19.389859950059712
39	19.12930192161546	32.0160677450874	27.825426120942353	21.029204212354795
40	20.26924329605906	33.27543154923461	28.56367386820106	17.891651286505265
41	19.976115514059277	31.082401476495498	29.975029855607428	18.966453153837804
42	19.83497991531864	32.645749647161004	28.867658234719357	18.651612202800997
43	17.72880251872761	33.75312126804907	28.856801650200847	19.661274563022474
44	17.924221040060797	32.244056019976114	29.68190207360764	20.149820866355444
45	18.564759526652917	32.51547063293888	29.88817717945934	19.031592660948863
46	20.323526218651615	31.01726196938443	28.444251438497446	20.214960373466507
47	20.128107697318423	31.885788730865272	27.749430029312776	20.236673542503528
48	18.662468787319508	33.785691021604606	26.60948865486918	20.94235153620671
49	18.423623927912278	31.364672673976767	30.02931277819998	20.182390619910976
50	17.54424058191293	33.861687113234176	26.989469113017044	21.604603191835846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	8.5
2	9.0
3	9.0
4	9.0
5	15.0
6	21.0
7	19.5
8	18.0
9	23.0
10	28.0
11	28.0
12	28.0
13	33.5
14	39.0
15	49.5
16	60.0
17	73.5
18	87.0
19	100.5
20	114.0
21	164.0
22	214.0
23	199.5
24	185.0
25	238.5
26	292.0
27	363.0
28	434.0
29	495.5
30	557.0
31	521.0
32	485.0
33	529.0
34	573.0
35	675.5
36	778.0
37	793.0
38	808.0
39	727.0
40	646.0
41	641.0
42	636.0
43	545.0
44	454.0
45	433.5
46	413.0
47	410.0
48	407.0
49	367.5
50	328.0
51	297.0
52	266.0
53	235.0
54	204.0
55	205.5
56	207.0
57	191.5
58	176.0
59	155.5
60	135.0
61	131.5
62	128.0
63	116.5
64	105.0
65	87.5
66	70.0
67	72.0
68	74.0
69	61.0
70	48.0
71	50.5
72	53.0
73	44.0
74	35.0
75	29.0
76	23.0
77	16.5
78	10.0
79	10.5
80	11.0
81	8.0
82	5.0
83	5.0
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	10.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03256975355553143
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9211.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.41830420149822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.42644790351984	69.23243947454131
2	5.132519983172066	7.9470198675496695
3	2.1455616323096343	4.983172293996309
4	1.051745898190997	3.256975355553143
5	0.5749544243444117	2.2255998262946477
6	0.3926518019913056	1.82390619910976
7	0.37862852334875896	2.05189447399848
8	0.12620950778291964	0.7816740853327543
9	0.1963259009956528	1.36792964933232
>10	0.5749544243444117	6.329388774291608
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.2822711974812724	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25	0.2714146129627619	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	22	0.2388448594072305	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	20	0.21713169037020952	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	19	0.20627510585169906	No Hit
GTCCAACTGTCTCTTATCTTTAATCAGTGAAATTGACCTTTCAGTGAAGA	19	0.20627510585169906	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.20627510585169906	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	18	0.19541852133318857	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	17	0.1845619368146781	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	17	0.1845619368146781	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	16	0.17370535229616763	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	16	0.17370535229616763	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	15	0.16284876777765714	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	15	0.16284876777765714	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG	15	0.16284876777765714	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	15	0.16284876777765714	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT	14	0.15199218325914668	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	14	0.15199218325914668	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	14	0.15199218325914668	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	14	0.15199218325914668	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	14	0.15199218325914668	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA	13	0.1411355987406362	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	13	0.1411355987406362	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	12	0.13027901422212573	No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT	12	0.13027901422212573	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC	11	0.11942242970361525	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	11	0.11942242970361525	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	11	0.11942242970361525	No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA	11	0.11942242970361525	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	11	0.11942242970361525	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA	11	0.11942242970361525	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	11	0.11942242970361525	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	11	0.11942242970361525	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.11942242970361525	No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC	10	0.10856584518510476	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	10	0.10856584518510476	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	10	0.10856584518510476	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT	10	0.10856584518510476	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	10	0.10856584518510476	No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT	10	0.10856584518510476	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	10	0.10856584518510476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.010856584518510477	0.0
24	0.0	0.0	0.0	0.021713169037020953	0.0
25	0.0	0.0	0.0	0.03256975355553143	0.0
26	0.0	0.0	0.0	0.03256975355553143	0.0
27	0.0	0.0	0.0	0.054282922592552386	0.0
28	0.0	0.0	0.0	0.054282922592552386	0.0
29	0.0	0.0	0.0	0.07599609162957334	0.0
30	0.0	0.0	0.0	0.10856584518510477	0.0
31	0.0	0.0	0.0	0.17370535229616763	0.0
32	0.0	0.0	0.0	0.30398436651829336	0.0
33	0.0	0.0	0.0	0.3582672891108457	0.0
34	0.0	0.0	0.0	0.44511996525892955	0.0
35	0.0	0.0	0.0	0.6296819020736076	0.0
36	0.0	0.0	0.0	0.8142438388882858	0.0
37	0.0	0.0	0.0	1.0965150363695582	0.0
38	0.0	0.0	0.0	1.4547823254804038	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACA	30	0.0054573826	29.333336	2
TAGAACA	40	6.5125234E-4	27.5	4
GTAGAAC	45	0.0012956869	24.444445	3
AGTTACA	45	0.0012956869	24.444445	24
TGTAGAA	50	0.0023926734	22.0	2
>>END_MODULE