FastQCFastQC Report
Thu 2 Feb 2017
SRR4062598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902104
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25950.13642787145182386No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19730.10372724099208036No Hit
GCGCAAGACGGACCAGAGCGAAAGC19490.10246548033125424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1000.017.10341315
CGTTATT1800.014.7663392
GCGTTAT1900.013.9873231
ACAGTCG707.2614584E-613.5719938
GTTATTC2250.013.5034943
CAGTGCG957.380913E-813.0012259
CGACCAT3200.012.46992510
TCGCGTA1551.8189894E-1212.2592199
ATCCCCG2650.012.19079112
TAGAGTG2050.012.0480135
ATCGCCA2700.011.96692616
CGCATCG2800.011.87705713
CGGTCCA3050.011.52567610
TATTAGC1657.2759576E-1211.5062382
CGGAATT1001.9255021E-611.40227515
AATCGTA752.0709426E-411.40197613
CAAGACG3350.011.3401724
GTATTAG3350.011.3330131
GTCGCGT1604.5474735E-1111.2817188
AATCCCC2800.011.19659911
Produced by FastQC (version 0.11.5)